ThermoFisher Scientific Microliter 48 x 2 sealed Instruction Manual
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ThermoFisher Scientific Microliter 48 x 2 sealed Instruction Manual

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Thermo Scientific
Microliter 48 x 2
sealed
Instruction Manual
50120031-e • 05 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

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Summary of Contents for ThermoFisher Scientific Microliter 48 x 2 sealed

  • Page 1 Thermo Scientific Microliter 48 x 2 sealed Instruction Manual 50120031-e • 05 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...

  • Page 5: Table Of Contents

    Rotor Loading ..........................4-1 Before Run .......................4-2 Proper Loading ......................4-2 Improper Loading ....................4-3 Maximum Loading ....................4-3 Cycle Counter ......................4-4 Chapter 5 Aerosol-tight Applications ....................5-1 Basic Principles ......................5-2 Fill Level ........................5-2 Checking the Aerosol-Tightness ................5-2 Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 6 Maintenance and Care ......................6-1 Cleaning intervals ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-6 RCF-Values .......................A-1 Chemical Compatibility Chart ................B-1 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 7: Preface

    If any parts are missing, please contact the nearest Thermo Fisher Scientific representative. Precautions In order to ensure safe operation of the Microliter 48 x 2 sealed, the following general safety regulations must be followed: • Do not remove the magnet at the rotor bottom.
  • Page 8 WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 9 Rotor Specifications Contents “Technical Data” page 1-2 • Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 10 Accel. / braking time [s] 45 / 45 Sample heating at n [°C] referred to ambient temperature of 23 °C, running time 120 minutes Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 11 RCF value at n 25,314 max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 12 Accel. / braking time [s] 45 / 45 Sample heating at n [°C] referred to ambient temperature of 23 °C, running time 120 minutes Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 13 Maximum RCF value at n 25,314 Max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 14 Accel. / braking time [s] 45 / 45 Sample heating at n [°C] referred to ambient temperature of 23 °C, running time 120 minutes Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 15 Maximum RCF value at n 25,314 Max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 16 Accel. / braking time [s] 45 / 45 Sample heating at n [°C] referred to ambient temperature of 23 °C, running time 120 minutes Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 17 Maximum RCF value at n 25,314 Max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 18 Accel. / braking time [s] 45 / 40 Sample heating at n [°C] referred to ambient temperature of 23 °C, running time 120 minutes Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-10 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 19 25,314 Max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed 1-11...
  • Page 20 9.8 / 5.9 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-12 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 21 25,314 Max. / min. radius [cm] 9.8 / 5.9 Pitch angle [°] Accel. / braking time [s] 45 / 45 Aerosol-tight Permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003602 Microliter 48 x 2 sealed 1-13...
  • Page 23 Accessories Contents “Rotor data” page 2-2 • “Accessories” page 2-2 • Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 24: Chapter 1 Rotor Specifications

    Accessories Microliter 48 x 2 Sealed Rotor Thermo Scientific General Purpose Rotors Microliter 48 x 2 Fixed Angle Aluminium Sealed Rotor Rotor Specifications Capacity (ml) 48 x 2 Tube Dimensions (mm) 11 x 50 Angle Maximum Speed (rpm) 15,200 K Factor...

  • Page 25: Autolock

    ™ AutoLock Contents “Rotor Installation” page 3-2 • “Removing the Rotor” page 3-3 • Thermo Scientific 75003602 Microliter 48x2 sealed...

  • Page 26: Rotor Installation

    AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 27: Removing The Rotor

    CAUTION Rotors supplied with a lid for aerosol-tight applications come with a mandril, ™ which belongs to the AutoLock . Be sure not to place the lid onto this mandril to prevent it from being damaged. Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 28 ™ AutoLock Aerosol-tight rotors ™ Figure 3-3. AutoLock -lid for aerosol-tight rotors ™ WARNING Mind the AutoLock -mandril inside the lid. Do not touch. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 29: Rotor Loading

    Rotor Loading Contents “Before Run” page 4-2 • “Proper Loading” page 4-2 • “Improper Loading” page 4-3 • “Maximum Loading” page 4-3 • Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 30: Before Run

    B-1. 5. Make sure the tubes or bottles do not touch the lid. Proper Loading To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 31: Improper Loading

    There are two options available for centrifuging samples whose weight, including adapter, exceeds the maximum permissible load: • Reduce the fill level. • Reduce the speed. Use the table or the formula: Actual Load (g/cavity) 15200 14493 13876 13331 Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 32: Cycle Counter

    Service life examples Usage profile Maximum service life at 30,000 cycles frequent use 7 years 20 runs / day 220 days / year 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 33: Aerosol-Tight Applications

    Aerosol-tight Applications Contents “Basic Principles” page 5-2 • “Fill Level” page 5-2 • “Checking the Aerosol-Tightness” page 5-2 • Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 34: Basic Principles

    This releases the carbonic acid gas, which is bound in the water, resulting in excess pressure. Do not apply pressure to the lid when doing so. Leaks can be detected by escaping water or the sound of escaping gas. 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 35 Damaged seals are to be replaced immediately. When loading the rotor, ensure that the rotor lid closes securely. Damaged or clouded rotor covers are to be replaced immediately. Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 37: Maintenance And Care

    Maintenance and Care Contents “Cleaning intervals” page 6-2 • “Cleaning” page 6-2 • “Disinfection” page 6-3 • “Decontamination” page 6-4 • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-6 • Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 38: Cleaning Intervals

    • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 39: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 40: Decontamination

    For general radioactive decontamination, use a solution of equal parts of 70 % ethanol, 10 % SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 41: Autoclaving

    Note No chemical additives are permitted in the steam. CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced. Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 42: Service Of Thermo Fisher Scientific

    • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Fisher Scientific offers inspection and service contracts for this work. 75003602 Microliter 48 x 2 sealed Thermo Scientific...

  • Page 43: Rcf-Values

    RCF-Values Speed (rpm) RCF R RCF R 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 2500 2600 2700 2800 2900 3000 Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 44 2143 3560 5800 2219 3686 5900 2296 3814 6000 2375 3944 6100 2454 4077 6200 2536 4212 6300 2618 4349 6400 2702 4488 6500 2787 4629 6600 2873 4773 6700 2961 4918 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 45 5828 9681 9500 5953 9888 9600 6079 10097 9700 6206 10309 9800 6335 10523 9900 6465 10738 10000 6596 10956 10100 6729 11177 10200 6863 11399 10300 6998 11624 10400 7134 11850 Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 46 11320 18802 13200 11493 19090 13300 11668 19381 13400 11844 19673 13500 12022 19968 13600 12200 20265 13700 12380 20564 13800 12562 20865 13900 12745 21169 14000 12929 21475 14100 13114 21782 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 47 13301 22092 14300 13489 22405 14400 13678 22719 14500 13869 23036 14600 14060 23355 14700 14254 23676 14800 14448 23999 14900 14644 24324 15000 14841 24652 15100 15040 24982 15200 15240 25314 Thermo Scientific 75003602 Microliter 48 x 2 sealed...

  • Page 49: Chemical Compatibility Chart

    M S M - Benzyl Alcohol Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 50 S S S U S U S S S U ® Ficoll-Hypaque M S S - S S S - S S S S - S S - S S S S S S S M S S S 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 51 S S S U S S M S S S S S U S U M S S S S S S S M S M U Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 52 S S S S S S S S S S S S S S S S S S S Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 53 Citric Acid (10 %) M S S M S S M S S S S S S S S S M S S S S S S S S S S Polyethyleneterephthalate Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 54 Chemical resistance data is included only as a guide to product use. Because, no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003602 Microliter 48 x 2 sealed Thermo Scientific...
  • Page 55 Scope of Supply ..........v Cycle Counter ..........4-4 Service .............6-5 Decontamination ..........6-4 Disinfection ............ 6-2 Fill Level ............5-2 Improper Loading ........... 4-3 Intervals ............6-2 Maintenance ........... 6-1 Maximum Loading ......... 4-3 Thermo Scientific 75003602 Microliter 48 x 2 sealed...
  • Page 57 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

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