Table of Contents
Related Manuals for ThermoFisher Scientific Applied Biosystems Veriti 4375305
Summary of Contents for ThermoFisher Scientific Applied Biosystems Veriti 4375305
- Page 1 ™ Veriti Thermal Cycler USER GUIDE Catalog Numbers 4375305, 4375786, 4384638, 4388444, 4413751, 4413964, and ZGEXSCVERTI3Y Publication Number MAN0018441 Revision B.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.
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Page 3: Table Of Contents
Contents ■ CHAPTER 1 System overview ........... . . 8 ™... - Page 4 Contents Connecting multiple instruments ..........29 Powering on .
- Page 5 Contents ■ CHAPTER 4 Utilities ............. . . 65 ™...
- Page 6 Contents Touchscreen specifications ..........90 Sample well specifications .
- Page 7 Contents ■ APPENDIX G Documentation and support ........105 Customer and technical support .
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Page 8: Chapter 1 System Overview
System overview ™ Introducing the Veriti Thermal Cycler About the instrument ™ There are four models of the Veriti Thermal Cycler: • Veriti ™ 96-Well Fast Thermal Cycler (PN 4375305), with 0.1–mL sample wells ™ • Veriti 96-Well Thermal Cycler (PN 4375786), with 0.2–mL sample wells •... -
Page 9: Using The Touchscreen
Chapter 1 System overview Using the touchscreen Using the touchscreen You interact with the instrument using a touchscreen. Table 1 describes several buttons common to many of the touchscreens. Table 1 Buttons in the instrument touchscreen Button Function Returns to the Main Menu screen. Returns to the previous screen. -
Page 10: Entering Text
Chapter 1 System overview Using the touchscreen Entering text When you touch a field that requires the input of text, the text editor opens (Figure 2). Figure 2 The instrument text editor To… Select… Enter a lowercase letter A letter button. Enter an uppercase letter Caps, then the letter(s). -
Page 11: Entering Numerals
Chapter 1 System overview Using the touchscreen Entering numerals When you touch a field that requires only numerical input, the numeric editor opens (Figure 3). Figure 3 The instrument numeric editor To… Select… Enter a number A number button. Delete the last number Remove the entire contents of the field Close the editor and save any changes Veriti... -
Page 12: Menu Overview
Chapter 1 System overview Menu overview Menu overview Main Menu screen After you log in to the system, the Main Menu screen opens. This screen is the starting point for operating the instrument. The date, current time, and current user are shown at the bottom of the screen. -
Page 13: Settings Menu Screen
Chapter 1 System overview Menu overview Settings Menu screen Use this screen to set various instrument configuration options. For instruments that require login, only Users and Administrators have access to this menu. Figure 5 The Settings Menu screen, with the Admin Menu button shown Admin Menu screen Use this screen to manage user accounts, set instrument security, back up run methods and settings, upgrade the firmware, and perform other functions. -
Page 14: Tools Menu Screen
Chapter 1 System overview About run methods Tools Menu screen Use this screen to access various tools and instrument tests. Figure 7 The Tools Menu screen, available to all users For more information about the functions on the Tools Menu, see Chapter 4, “Utilities” About run methods What is a run method? A run method is a set of instructions that specifies how the instrument should heat and cool your... -
Page 15: Viewing A Run Method
Chapter 1 System overview About run methods • Touchdown PCR Run Method • XL PCR Run Method Each method is stored in the Shared folder. You can edit, copy, and run these predefined methods as you would any other run method, but you cannot delete them. For details on these methods, see Appendix E, “Predefined run methods”... -
Page 16: Administrative Tasks For Run Methods
Chapter 1 System overview About run methods 2. Select one or more rows to choose a method(s). 3. Select a highlighted row to deselect it. Administrative tasks for run methods Table 2 Administrative tasks for run methods If you want to… Do this…... -
Page 17: Copying A Run Method
Chapter 1 System overview About run methods Table 2 Administrative tasks for run methods (continued) If you want to… Do this… Create a shortcut to a run method on the Main Menu Select a run method in the list of run methods, then select (Create Method Shortcut.) See page 19. -
Page 18: Deleting A Run Method
Chapter 1 System overview About run methods Deleting a run method Note: If your instrument requires log in, you must have User or Administrator level permissions to delete a run method. To delete a run method: 1. Log in as a user with User or Administrator access. 2. -
Page 19: Creating A Shortcut To A Run Method
Chapter 1 System overview About run methods Creating a shortcut to a run method You can add buttons to the Main Menu screen that allow you to go directly to the Edit Run Method screen for a particular run method. When login is required, each user can configure his or her own shortcut buttons;... - Page 20 Chapter 1 System overview About run methods 4. In the Admin Menu screen, select Back Up Methods & Settings. The Back Up Method and Settings screen is displayed: 5. Select Back Up. If a back up file already exists, you are prompted to overwrite it. The instrument copies all run methods, folders, user information, and other instrument settings to the USB drive, then displays a message box when the backup is complete.
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Page 21: Restoring Run Methods And Other Settings
Chapter 1 System overview About run methods Restoring run methods and other settings You can restore run methods and other settings from a USB drive to an instrument. The restore feature can also be used to load run methods onto another instrument in your lab. To restore run methods and instrument settings: 1. -
Page 22: About Folders
Chapter 1 System overview About folders About folders Each run method is saved in a folder. There are three folders available by default: • Default – Run methods are stored here if you do not select another folder. Run methods stored in this folder can be accessed, edited, run, and deleted only by the user who created them. - Page 23 Chapter 1 System overview About folders 5. Repeat steps 3 on page 22 and 4 on page 22 to rename any other folders. 6. When you are done making changes to folders, select (Close) to return to the Settings Menu screen.
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Page 24: Chapter 2 Getting Started
Getting started ™ Installing the Veriti Thermal Cycler Before you start Site requirements The instrument is intended for indoor use. Ensure that the installation site: • Meets the spatial and weight requirements (see page 24) • Meets environmental requirements (see page 25) •... -
Page 25: Environmental Requirements
Chapter 2 Getting started Installing the Veriti ™ Thermal Cycler Environmental requirements Temperature and humidity requirements Ensure that the installation site is maintained under the following conditions: Condition Acceptable Range Temperature 15 to 30°C (59 to 86°F) Humidity 20 to 80% relative humidity, noncondensing Avoid placing the instrument adjacent to heaters, cooling ducts, or in direct sunlight. - Page 26 Chapter 2 Getting started Installing the Veriti ™ Thermal Cycler Optional protective hardware ™ The following devices are supported to protect the Veriti Thermal Cycler from damage resulting from electrical hazards and the resultant loss of data. Before installing the system, decide what additional hardware (if any) you want to install.
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Page 27: Unpacking The Instrument
Chapter 2 Getting started Installing the Veriti ™ Thermal Cycler Unpacking the instrument IMPORTANT! Save the packing materials and box in case you need to ship the instrument for service. 1. To unpack the instrument: a. Cut the straps securing the instrument box. b. -
Page 28: Setting Up The Instrument
Chapter 2 Getting started Installing the Veriti ™ Thermal Cycler 2. Move the instrument to the desired installation site. Follow these guidelines for lifting and moving: • Make sure that you have a secure, comfortable grip. • Keep your spine in a neutral position. •... -
Page 29: Connecting Multiple Instruments
Chapter 2 Getting started Installing the Veriti ™ Thermal Cycler Connecting multiple instruments Required parts for connection Connecting your thermal cycler to other thermal cyclers allows you to use one instrument to control others. You can connect up to 12 instruments. To connect together multiple instruments, you need: •... -
Page 30: Powering On
Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler Powering on 1. Verify that the heated cover is closed. 2. Press the power button on the back left side of the instrument: The instrument powers on and the Main Menu screen is displayed: ™... - Page 31 Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler Optionally, you can also change the settings for: • User accounts and security (“Setting up instrument security and user accounts” on page • Idle state temperatures for the cover and the sample block(s) (“Setting idle temperatures for the block(s) and cover”...
- Page 32 Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler Setting the instrument name By default the instrument name is set to the instrument serial number. You can change the name to something more descriptive. The instrument name appears in the About the Instrument and Run Report screens and, if you connect together multiple instruments, in the Select Instruments screen.
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Page 33: Setting Up Instrument Security And User Accounts
Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler 5. To set the time, select Time, enter the current time using the format HH:MM, then select AM or PM. The instrument automatically enters the ":". 6. Select Done, then OK to save your changes and return to the Admin Menu screen. Setting up instrument security and user accounts By default, the instrument has all security settings disabled, allowing anyone to use the instrument and/or change instrument settings. -
Page 34: Adding Users
Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler 9. Select Done, then select either: • Yes, to restart the instrument and have the changes take effect immediately. Toggle the switch on the back of the instrument to power off then toggle the switch again to power on the instrument. - Page 35 Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler c. Log In. 3. In the Main Menu screen, select Settings Menu. 4. In the Settings Menu screen, select Admin Menu. 5. Follow the instructions in “Adding users” on page 35 to add yourself as a user.
- Page 36 Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler 6. Enter information for the new user. The required fields are User Name, Password, Re-enter Password, and Permission Level. a. User Name must be between 5 and 15 characters, with no spaces. b.
- Page 37 Chapter 2 Getting started Setting up the Veriti ™ Thermal Cycler 5. In the list, select the user in the list, then select Edit User. The Edit User Account screen is displayed: 6. In the Edit User Account screen, make the required changes: •...
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Page 38: Setting Other Options
Chapter 2 Getting started Setting other options 4. In the Admin Menu screen, select Manage Users. The Manage Users screen is displayed: 5. In the list, select the user name for the account you want to delete, then select Delete User. A confirmation message appears. -
Page 39: Setting Idle Temperatures For The Block(S) And Cover
Chapter 2 Getting started Setting other options Setting idle temperatures for the block(s) and cover 1. In the Main Menu screen, select Settings Menu. 2. In the Settings Menu screen, select Set Idle Temperatures.. 3. In the Idle State Temperatures screen, to set the temperatures for the sample block(s) and the cover, select: a. -
Page 40: Configuring Main Menu Shortcuts To Functions
Chapter 2 Getting started Setting other options 3. In the Set Time-Outs screen, select Time-out, then enter the duration. • Default Standby Time-Out sets the duration that the instrument is idle before the touchscreen is turned off. Enter a duration of 00:00 if you do not want the instrument to turn off the touchscreen. -
Page 41: About The Thermal Cycler Fleet Control Software
Chapter 2 Getting started About the Thermal Cycler Fleet Control Software About the Thermal Cycler Fleet Control Software ™ The Applied Biosystems Thermal Cycler Fleet Control Software (Cat. No. A40070) is used to manage users, methods, run reports, and multiple PCR instruments through a single user interface. The software ™... -
Page 42: Chapter 3 Performing A Run
Performing a run Selecting disposables Use this section to determine the tube, sample tray, and plate configurations appropriate for your thermal cycler and application. • “Disposables for 96‑well instruments” on page 42 • “Disposables for the 384-well instrument” on page 44 •... - Page 43 Chapter 3 Performing a run Selecting disposables 96-well sample tray and plate configurations The following table lists the possible sample tray and plate configurations. ™ IMPORTANT! The MicroAmp Splash-Free 96-Well Base shown below is used only when pipetting samples into tubes or trays. Do not place it in the sample block(s). Run Vessel Compatible Component Illustration...
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Page 44: Disposables For The 384-Well Instrument
Chapter 3 Performing a run Loading samples into the instrument Disposables for the 384-well instrument There is one plate configuration for the 384‑well instrument, shown in Figure 9. This uses the ™ MicroAmp Optical 384-Well Reaction Plate. Figure 9 MicroAmp ™... -
Page 45: Loading Samples In 96-Well Instruments
Chapter 3 Performing a run Loading samples into the instrument Loading Samples in 96-well instruments This section describes how to load samples in: • Tubes with attached caps • 96-well reaction plates • 96-well tray/retainer assemblies Loading tubes with attached caps There is no need to use mineral oil or glycerine in the sample wells or as a vapor barrier over the PCR ™... -
Page 46: Loading Samples In The 384-Well Instrument
Chapter 3 Performing a run Loading samples into the instrument Placing the sample tray or plate onto the sample block(s) The steps for placing the sample tray in the sample block(s) are the same for: • A sample tray/retainer • A sample tray without a retainer •... -
Page 47: Loading Samples In The Veriti ™ 60-Well Thermal Cycler
Chapter 3 Performing a run Creating a new run method 3. Place the MicroAmp ™ Optical 384-Well Reaction Plate onto the sample block(s) so that well A1 is at the upper left corner of the tray, as shown below: 4. Close the heated cover. ™... - Page 48 Chapter 3 Performing a run Creating a new run method 3. Edit the default run method as needed: • Insert a stage (see “Adding a stage” on page 49). • Insert a step (see “Adding a step” on page 50). •...
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Page 49: Editing Details
Chapter 3 Performing a run Editing details 6. Select (Folder) to choose a folder in which to save the run method. • To save the run method so that all users have access to it, select Shared. • To create a new folder for the run method, select New Folder at the bottom of the list, enter a name of up to 12 characters (no spaces), for the new folder, then select Done. -
Page 50: Adding A Step
Chapter 3 Performing a run Editing details 1. In the Edit Run Method screen, select the stage name (at the top of the stage). When a stage is selected, the area around the name is red. 2. Touch Add. A copy of the selected stage is added to the run method. 3. -
Page 51: Editing Temperature And Time
Chapter 3 Performing a run Editing details 2. Touch Add. A copy of the selected step is added to the run method: 3. To change the temperature and/or time for the step, see “Editing temperature and time” page Editing temperature and time 1. -
Page 52: Editing Ramp Rates
Chapter 3 Performing a run Editing details c. Repeat for any other field. 3. Select Done to close the editor. Editing ramp rates You can edit the rate at which the instrument ramps to the temperature for the step. The default ramp rate is 100%. - Page 53 Chapter 3 Performing a run Editing details 2. In the options menu, select AutoDelta. The Create an AutoDelta Step screen is displayed: 3. Select Starting Cycle, then enter the cycle at which the AutoDelta step begins. 4. Select the parameter(s) to increment: •...
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Page 54: Adding A Pause
Chapter 3 Performing a run Editing details Adding a pause You can add a pause before any step in any cycling stage. 1. In the Edit Run Method screen, select a step in a cycling stage, then touch Options. 2. In the options menu, select Pause to display the Add a Pause Before a Step dialog box: 3. -
Page 55: Deleting A Stage Or Step
Chapter 3 Performing a run Editing details Deleting a stage or step 1. In the Edit Run Method screen, select the stage or step to select it. 2. Select Delete, then OK. The stage or step is deleted from the run method. Creating a VeriFlex ™... - Page 56 Chapter 3 Performing a run Editing details 3. Select a temperature field, then enter the desired temperature. Temperatures for neighboring ™ VeriFlex Blocks can be the same, but if they are not the same, they must be at least 0.1°C apart but no more than 5.0°C apart.
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Page 57: Performing A Run
Chapter 3 Performing a run Performing a run Performing a run Before you begin Prepare your samples and load the sample block(s) as described in “Loading samples into the instrument” on page IMPORTANT! You can start a run on multiple instruments at the same time if your instruments are on a network. - Page 58 Chapter 3 Performing a run Performing a run 7. Select Run ID, and/or Notes, then enter the applicable information. The default Run ID is YYYYMMDDHHMM, where “YYYYMMDD” is the date and “HHMM” is the current time. 8. If you are making runs on: •...
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Page 59: Monitoring A Run
Chapter 3 Performing a run Monitoring a run 10. While the cover heats, the Cover Heating dialog box appears in the Run Monitor screen: If you want to: • Start the run immediately, before the cover reaches the specified temperature – Select (Skip). -
Page 60: Actions In The Run Monitor Screen During A Run
Chapter 3 Performing a run Monitoring a run Table 5 Elements of the Run Monitor screen Location in Element Description Figure Run Monitor Displays the estimated temperature of the sample, the temperature of button and the heated cover, the estimated time remaining in the run, and the status bar current stage. -
Page 61: Changing The Number Of Cycles
Chapter 3 Performing a run Monitoring a run Table 6 Actions in the Run Monitor screen allowed during a run (continued) To… Do This Skip to the next step Select SKIP. Skip works only when the instrument is holding. Stop the run Select STOP (see page 62). -
Page 62: Stopping A Run
Chapter 3 Performing a run Monitoring a run The run pauses for the duration set in the Set Time-Outs screen (see “Setting time-outs” page 39). In the Pause Run dialog box: • Remaining Time shows the remaining time for the pause. •... -
Page 63: If The Power Fails
Chapter 3 Performing a run Viewing and printing the run report Figure 12 Alert icon, shown in the Run Monitor button when an error occurs If the power fails If a power failure occurs during a run, the instrument attempts to continue the run when the power returns. -
Page 64: Removing The Samples From The Instrument
Chapter 3 Performing a run Removing the samples from the instrument Removing the samples from the instrument Grasp the sample tray/retainer assembly or reaction plate firmly with both hands and gently rock it back and forth until the tubes (or plate) are free. CAUTION! Sample caps may pop off if the cover is opened when the sample block(s) temperature is above 27°C. -
Page 65: Chapter 4 Utilities
Utilities ™ Using GeneAmp PCR System 9700 run methods ™ Note: This function is only available on Veriti 96-Well Thermal Cyclers. ™ The Veriti Thermal Cycler has a Convert Method tool to guide you through importing existing run ™ methods from a GeneAmp PCR System 9700 thermal cycler. - Page 66 Chapter 4 Utilities Using GeneAmp ™ PCR System 9700 run methods 4. Select the check box next to the run method’s original format (9700 Max ™ Mode or 9600 Emulation Mode) then select (Next). 5. In the second page, enter the original run method. You can include AutoDelta steps, pauses, and ™...
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Page 67: Upgrading System Firmware
Chapter 4 Utilities Upgrading system firmware Figure 13 Converted run method, showing “9700M” as the ramp rate Upgrading system firmware About the upgrade If a new version of the firmware is available for the instrument, you may be required to load the new firmware on the instrument. - Page 68 Chapter 4 Utilities Upgrading system firmware b. Record the version number of the firmware, then select Done. 2. Determine the version number of the new firmware. a. On a computer connected to the Internet, go to www.appliedbiosystems.com. b. Click Support, then Software Downloads. c.
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Page 69: Calculating Melting Temperatures
Chapter 4 Utilities Calculating melting temperatures 7. Select Source File to display a list of the files on the USB drive. The instrument firmware files end with “.update”. 8. Select the name of the file with the new firmware, then touch Select. 9. -
Page 70: Viewing Instrument Statistics
Chapter 4 Utilities Viewing instrument statistics Viewing instrument statistics The instrument records information about how it has been used. You can view this information. 1. In the Main Menu screen, select Tools Menu. 2. In the Tools Menu screen, select Show Statistics. The following statistics are shown: •... -
Page 71: Running The Cycle Performance Test
Chapter 4 Utilities Running the cycle performance test 4. In the Admin Menu screen, select Restore Factory Settings to open the Restore Factory Settings screen: 5. In the Restore Factory Settings screen, select Restore. The Restore Factory Settings dialog box is displayed. 6. -
Page 72: Required Materials
Chapter 4 Utilities Tests using the temperature verification kit Required materials The appropriate equipment for your instrument: ™ ™ • Veriti 96-Well Fast Thermal Cycler: MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (PN 4346906) ™ ™ • Veriti 96-Well Thermal Cycler: MicroAmp Optical 96-Well Reaction Plate (PN N8010560) ™... -
Page 73: About The Temperature Verification Kit
Chapter 4 Utilities Tests using the temperature verification kit About the temperature verification kit The Temperature Verification Kit consists of: • A probe – The appropriate size for the sample wells in your instrument. The probe can be ordered separately or a part of a kit. (See “Parts list” on page 81 for part numbers for the probes.) •... -
Page 74: Running The Temperature Verification
Chapter 4 Utilities Tests using the temperature verification kit Procedure This test takes 10 to 15 minutes to complete. WARNING! PHYSICAL INJURY HAZARD. Hot Surface. Use care when working around the heated cover and sample block(s) to avoid being burned. 1. -
Page 75: Running The Temperature Nonuniformity Test
Chapter 4 Utilities Tests using the temperature verification kit Procedure This test takes 25 to 30 minutes to complete, but you can cancel before it is complete. WARNING! PHYSICAL INJURY HAZARD. Hot Surface. Use care when working around the heated cover and sample block(s) to avoid being burned. -
Page 76: Tvk Recalibration
Chapter 4 Utilities Tests using the temperature verification kit 4. At the end of the test, select: • Print to print the results. • Save to save the results to the USB drive. The results are saved to the file tnuEvents.txt. 5. -
Page 77: Chapter 5 Maintenance
Maintenance Scheduled maintenance Run the following tests annually: • Cycle performance test—See “Running the cycle performance test” on page • Heated cover verification—See “Running the heated cover verification” on page • Temperature verification—See “Running the temperature verification” on page • Temperature nonuniformity test—See “Running the temperature nonuniformity test” on page As-Needed maintenance Tests... - Page 78 Chapter 5 Maintenance As-Needed maintenance Cleaning the sample wells If you use any cleaning or decontamination method, except those recommended in the manual, you risk damaging the equipment. Clean the sample wells once a month or as needed. WARNING! Always wear protective glasses and gloves when servicing the instrument. Also, make sure you disconnect the instrument from AC line power before you begin any service procedure.
- Page 79 Chapter 5 Maintenance As-Needed maintenance Cleaning the heated cover Clean the heated cover once a month or as needed. CAUTION! During instrument operation, the temperature of the heated cover can be as high as 108°C, and the temperature of the sample block(s) can be as high as 100°C. Before performing the procedure, keep hands away until the heated cover and sample block(s) reach room temperature.
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Page 80: Replacing Fuses
Chapter 5 Maintenance As-Needed maintenance Replacing fuses Fuse overview The instrument has two 10 A Type M 250 V 5 × 20 mm fuses (Cat. No. 4377690). WARNING! FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with Listed and Certified fuses of the same type and rating as those currently in the instrument. -
Page 81: Parts List
Chapter 5 Maintenance Parts list 2. Insert a small flat-tip screwdriver into the slot in the upper portion of the power entry module, and open the door. 3. Pull out the fuse compartment to inspect the two fuses in the fuse compartment: 4. - Page 82 Chapter 5 Maintenance Parts list Table 7 Kits and spares (continued) Part Foot, Rubber (Package of 4) 4375246 Fuse 10A Slo-Blo 4377690 Grill, Plastic Mold 4374178 Handle, Plastic 4374277 Handle, Rubber Grip 4374181 Heated Cover 4374276 Panel, Left 4374176 Panel, Rear (Heated Cover) 4374280 Panel, Right 4374175...
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Page 83: Appendix A Troubleshooting
Troubleshooting Observation Possible cause Recommended action Cooling rate too slow. Peltier assembly failed. Run the Cycle Performance Test. Temperature does not match Instrument may require Run the Temperature Verification Test. specified temperature. calibration. Heating rate too slow. Peltier failed. Run the Cycle Performance Test. Printing fails. - Page 84 Appendix A Troubleshooting Parts list Observation Possible cause Recommended action Cannot see Manage Users ™ Manage Users button is not Use VeritiLink Software to manage user button in Admin Menu screen. shown when instrument is accounts. ™ monitored byVeritiLink Remote Change Allow VeritiLink ™...
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Page 85: Appendix B Returning An Instrument For Service
Returning an instrument for service Before returning an instrument for service, back up the run methods and instrument settings, as described in “Backing up run methods and other settings” on page 19. This practice ensures that if the service center sends you a replacement instrument, you can restore the run methods and other settings. -
Page 86: Appendix C Disposables
Disposables Disposables ™ Table 8 Disposables for the Veriti thermal cycler 96-Well 96-Well 384-Well 60-Well Part Part Description (0.1‑mL) (0.2‑mL) (0.02‑mL) (0.5‑mL) Number 96-Well Plates ™ MicroAmp Fast 96-Well Reaction Plate (0.1 mL) 4346907 MicroAmp ™ Fast Optical 96-Well Reaction Plate 4346906 with Barcode (0.1 mL) 4366932... - Page 87 Appendix C Disposables Disposables Table 8 Disposables for the Veriti thermal cycler (continued) 96-Well 96-Well 384-Well 60-Well Part Part Description (0.1‑mL) (0.2‑mL) (0.02‑mL) (0.5‑mL) Number ™ MicroAmp 8-Tube Strip (0.2 mL) N8010580 ™ MicroAmp 8-Tube Strip, Assorted Colors (0.2 N8010838 ™...
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Page 88: Other Supplies For Sample Handling
Appendix C Disposables Other supplies for sample handling Table 8 Disposables for the Veriti thermal cycler (continued) 96-Well 96-Well 384-Well 60-Well Part Part Description (0.1‑mL) (0.2‑mL) (0.02‑mL) (0.5‑mL) Number Reaction Trays ™ ™ ™ MicroAmp 96-Well Tray for VeriFlex Blocks 4379983 ™... -
Page 89: Appendix D Instrument Specifications
Instrument specifications System specifications Physical dimensions and electrical specifications Physical Dimensions Width 23.7 cm (9.3 in) Height 24.5 cm (9.6 in) Depth 48.5 cm (19.1 in) Weight 10.2 kg (22.5 lbs) Electrical Input Voltage 100 to 240 VAC 50/60 Hz Max Power 10 A 100, 120, 220, 230 +10%... -
Page 90: Sample Temperature Range
Appendix D Instrument specifications System specifications ™ Figure 14 Connections for power and communications on the Veriti thermal cycler Sample temperature range • Sample temperature range: +4 to +99.9°C • Temperature calibration accuracy: Traceable to National Institute of Standards and Technology (NIST) Touchscreen specifications 640 ×... -
Page 91: Printer Specifications
Appendix D Instrument specifications Printer specifications Printer specifications The instrument requires a printer that: • Has an Ethernet port • Supports the LPD/LPR protocol (often called a “TCP/IP printer”) Veriti ™ Thermal Cycler User Guide... -
Page 92: Appendix E Predefined Run Methods
Predefined run methods Predefined run methods ™ The following run methods are supplied with the Veriti Thermal Cycler and are stored in the Shared folder. These run methods are recommended protocols and should be considered as starting points for the different applications. Your results may vary between instrument models, so some optimization may be needed. -
Page 93: Amplicycle ™ Seq Run Method
Appendix E Predefined run methods Predefined run methods AmpliCycle ™ Seq Run Method Use this run method to achieve clean sequence ladders from femtomole quantities of template. The ™ cycle sequencing process and the benefits of AmpliTaq DNA Polymerase, CS, are described in the ™... -
Page 94: Bigdye ™ Kit Fast Run Method
Appendix E Predefined run methods Predefined run methods BigDye ™ Kit Fast Run Method ™ Use this run method for cycle sequencing of dideoxy (Sanger) terminator sequencing using ABI PRISM ™ BigDye Terminator Cycle Sequence Ready Reaction Kits. ™ BigDye Kit Standard Run Method Use this run method, which consists of cycle-sequencing parameters for dideoxy (Sanger) terminator ™... -
Page 95: General Pcr Run Method
Appendix E Predefined run methods Predefined run methods General PCR Run Method ™ Use this run method for standard PCR use. This method is set as the default run method for the Veriti Thermal Cycler. LMS2 Run Method ™ Use this run method for ABI PRISM Linkage Mapping Set Version 2.5 (LMS2) thermal cycling. -
Page 96: Touchdown Pcr Run Method
Appendix E Predefined run methods Limited product warranty Touchdown PCR Run Method Use this run method if the optimal annealing temperature is not known. This method incrementally decreases the annealing temperature in early cycles to maximize the yield of specific products. XL PCR Run Method Use this run method for amplification of 5 to 40 kb PCR products using rTth DNA Polymerase, XL and unique reaction conditions. -
Page 97: Appendix F Safety And Emc Compliance Information
Safety and EMC compliance information WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document. -
Page 98: Symbols On Instruments
Appendix F Safety and EMC compliance information Symbols on instruments ™ Except for IMPORTANTs, each safety alert word in an Applied Biosystems document appears with an open triangle figure that contains a hazard symbol. These hazard symbols are identical to the hazard ™... -
Page 99: Safety Symbols
Appendix F Safety and EMC compliance information Symbols on instruments Safety symbols ™ The following table describes the safety symbols that may be displayed on Applied Biosystems instruments. Each symbol may appear by itself or with text that explains the relevant hazard (see “Safety labels on instruments”... -
Page 100: General Instrument Safety
Appendix F Safety and EMC compliance information General instrument safety (continued) English Francais AVERTISSEMENT Lampe brûlante. WARNING! Hot lamp. AVERTISSEMENT Composants brûlants. Remplacer WARNING! Hot. Replace lamp with an ™ la lampe par une lampe Applied Biosystems Applied Biosystems ™ lamp. -
Page 101: Cleaning Or Decontaminating The Instrument
Appendix F Safety and EMC compliance information Electrical safety Cleaning or decontaminating the instrument CAUTION! Before using a cleaning or decontamination method other than those recommended by the manufacturer, verify with the manufacturer that the proposed method will not damage the equipment. -
Page 102: Safety And Electromagnetic Compatibility (Emc) Standards
Appendix F Safety and EMC compliance information Safety and electromagnetic compatibility (EMC) standards Safety and electromagnetic compatibility (EMC) standards Symbol Description U.S. and Canadian safety standards. ™ The Veriti Thermal Cycler has been tested to and complies with standard: UL 61010A-1/CAN/CSA C22.2 No. 1010.1-92, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements.”... -
Page 103: Chemical Safety
Appendix F Safety and EMC compliance information Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: ·... -
Page 104: Biological Hazard Safety
Appendix F Safety and EMC compliance information Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. - Page 105 Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •...
- Page 106 Veriti Thermal Cycler_UG_MAN0018441-v2-GUID-C91D3CB1-6A23-45D6-AFB6-561C237FA546-2019/07/15 14:17:20 en 02:20:59.581+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 17 June 2021...
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