Switching On Protocol; Objective Lens - Leica TCS SP8 Safety Manual

4.1 Switching ON Protocol

(a) SIGN IN the Logbook and record your START-TIMING.
(b) Switch on Laser Key (1), Laser Power (2), Scanner power (3) and PC Microscope (4).
(c) Switch on the Metal Halide lamp (5), if you need it.
(d) Switch on STED laser module (6), if you need it.
(e) Wait for PC to start up and log in with your TLL user name.
(f) Load LASx software and switch on the lasers that you are planning to use:
• Diode Laser: "ON" 405nm laser
• White Light laser: "ON" up to 70% power
• Argon laser: "ON" up to 70% power. (Make sure the argon lasers are ON for a
minimum of 2hrs before switching OFF as it requires sufficient time for warming up)
• STED laser: You may switch "ON" up to 100%. (Make sure the STED lasers are ON for
at least 1hr before switching OFF.)
(g) Do not overload the HyD detectors. Keep gain at max 100% as overloading will damage
the performance of HyD detectors over time. For best performance of the HyD detectors,
set gain at 100% and only use the laser power slider to moderate irradiation intensity.
(h) Finally, fill in the logbook.

4.2 Objective Lens

Objective lens
10x/0.4 HC Plan Apochromat
20x/0.75 HC Plan Apochromat
40x/1.3 HCX Plan Apochromat
63x/1.2 HC Plan Apochromat
63x/1.4 HC Plan Apochromat
100x/1.4 HCX Plan Apochromat
Immersion media
Air
Air
Air
Water
Oil
Oil
Last Updated on Sep 2021