Electrode Preparation; Preparation For Refillable Ross Electrodes - Thermo Scientific Orion ROSS Ultra User Manual

Ph electrodes
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Note: The 8107BNUMD, 8107UWMMD and 8107BNURCA
electrodes are gel-filled and do not require any fill solution.
4. ROSS pH electrode storage solution, Cat. No. 810001, or standard
pH electrode storage solution, Cat. No. 910001. For enhanced
electrode performance, use ROSS pH electrode storage solution.
5. pH buffers – at least two pH buffers are recommended for precise
measurements. One buffer should be near pH 7 and buffers should
be one to four pH units apart.
6. Beakers, plastic or glass.
7. Magnetic stirrer or Orion stirrer probe, Cat. No. 096019. The stirrer
probe can be used with Orion Star A series, V
s
benchtop meters.
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8. Distilled or deionized water.

Electrode Preparation

Preparation for Refillable ROSS Electrodes

1. Remove the shipping cap, sleeve or bottle from the sensing bulb
and save it for later storage of the electrode.
2. Clean any salt deposits from the exterior of the electrode by rinsing
it with distilled water. Handle the 8220BNWP ROSS micro pH
electrode with great care.
3. The electrode may arrive with the chamber partially filled with
electrolyte solution. Please fill the chamber with electrolyte solution
before use. Uncover the fill hole by removing the tape and add
ROSS fill solution, Cat. No. 810007, to the electrode up to the fill
hole. To maintain an adequate flow rate, the level of fill solution
must always be above the reference junction and at least one inch
above the sample level. The fill hole should remain open whenever
the electrode is in use.
4. Place the electrode in an electrode holder and suspend the
electrode in air for 5 minutes to thoroughly wet the reference
junction. Omit this step for electrodes which come shipped in a
storage bottle.
5. Shake the electrode downward (similar to a clinical thermometer)
to remove any air bubbles. The 8220BNWP electrode is extremely
fragile. Do not shake the 8220BNWP electrode unless there are
air bubbles in the fill solution and then shake gently and with great
care.
6. Soak electrode in ROSS pH electrode storage solution,
Cat. No. 810001, or standard pH electrode storage solution, Cat.
No. 910001, for 30 to 60 minutes. ROSS Ultra Triode electrodes
do not need to be soaked prior to use, since they are shipped in a
storage bottle with storage solution.
7. Connect the electrode to the meter.
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ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes
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