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Sigma 8KS Operating Manual

Refrigerated centrifuge
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Original operating manual
sb
08/2012
Rev. 2.14 - 2025-03-05
Sigma 8KS; ;
part no. 0702502
Sigma 8KS
from serial no. 162923
Refrigerated Centrifuge
Operating Manual
Please retain for later use!

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  • Page 1 Original operating manual 08/2012 Rev. 2.14 - 2025-03-05 Sigma 8KS; ; part no. 0702502 Sigma 8KS from serial no. 162923 Refrigerated Centrifuge Operating Manual Please retain for later use!
  • Page 2 Refrigerated Centrifuge Sigma 8KS Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 2 / 129 Original operating manual, part no. 0702502...
  • Page 3 Refrigerated Centrifuge Sigma 8KS © Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel.: +49 (0) 5522 / 5007-0 Fax: +49 (0) 5522 / 5007-12 Web: www.sigma-zentrifugen.de E-mail: info@sigma-zentrifugen.de Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 3 / 129 Original operating manual, part no.
  • Page 4 Refrigerated Centrifuge Sigma 8KS Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 4 / 129 Original operating manual, part no. 0702502...

  • Page 5: Table Of Contents

    Refrigerated Centrifuge Sigma 8KS Table of contents General information ......................9 Importance of the operating manual .................. 9 Further applicable documents ................... 9 Intended use ........................9 Warranty and liability....................... 10 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................11 Layout and mode of operation ..................
  • Page 6 Refrigerated Centrifuge Sigma 8KS Table of contents Safety devices ........................ 31 3.7.1 Lid lock device ......................31 3.7.2 Standstill monitoring system ..................31 3.7.3 System check ......................31 3.7.4 Earth conductor check ....................31 3.7.5 Imbalance monitoring system ..................31 3.7.6...
  • Page 7 Refrigerated Centrifuge Sigma 8KS Table of contents 6.3.3 Program mode ......................66 6.3.3.1 Saving a program ....................67 6.3.3.2 Loading a program ..................... 67 6.3.3.3 Executing a program ..................68 6.3.3.4 Deleting a program .................... 68 6.3.3.5 Automatic program rotation ................69 6.3.4...
  • Page 8 Refrigerated Centrifuge Sigma 8KS Table of contents Appendix ..........................88 11.1 Range of accessories ..................... 88 11.1.1 Rotor radii ........................88 11.2 Speed-gravitational-field-diagram ................... 89 11.3 Acceleration and deceleration curves ................90 11.4 Table of the service life of rotors and accessories ............92 11.5...

  • Page 9: General Information

    They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use.

  • Page 10: Warranty And Liability

    Refrigerated Centrifuge Sigma 8KS 1 General information → Warranty and liability The warranty and liability are subject to our "General Conditions" that were distributed to the operator upon the conclusion of the contract. Warranty and liability claims are excluded if they are due to: •...

  • Page 11: Scope Of Supply

    Refrigerated Centrifuge Sigma 8KS 1 General information → Scope of supply The centrifuge comprises: • 1 square spanner, size 8 (door) Part no. 930 114 • 1 open spanner, size 8/10 Part no. 930 015 (adjustable feet front side) •...

  • Page 12: Layout And Mode Of Operation

    Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation → Layout and mode of operation → Layout of the centrifuge → 2.1.1 Functional and operating elements User interface (see chapter 6.3.1 - "User interface") Fig. 1: Total view of the centrifuge Version 08/2012, Rev.
  • Page 13 Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation Locks of the front door Name plate (see chapter 2.1.2 - "Name plate") Fig. 2: Right side of the centrifuge Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 13 / 129...
  • Page 14 Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation Mains power switch Condensate drain Fig. 3: Left side of the centrifuge Mains cable Option: connector for barcode scanner RS-232 interface 10 Option: serial interface (see chapter 6.3.4 - "Options for data input and output")
  • Page 15 Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation 13 Cooling water connection Fig. 5: Rear view of a water cooled centrifuge Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 15 / 129 Original operating manual, part no. 0702502...

  • Page 16: Name Plate

    Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation → 2.1.2 Name plate Manufacturer Sigma 8KS: Power consumption Max. speed Max. kinetic energy Product name Part number Serial number Nominal voltage Product designation 10 CE mark in compliance with the...

  • Page 17: F-Gases Label

    Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation 2.1.3 F-gases label Fig. 7: F-gases label in compliance with the implementation regulation (EU) 2024/2729 Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 17 / 129 Original operating manual, part no. 0702502...

  • Page 18: Mode Of Operation

    Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation → Mode of operation → 2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components. The mixture of substances, which rotates on a circular path, is subject to centripetal acceleration that is several times greater than the gravitational acceleration.

  • Page 19: Speed, Radius, And Relative Centrifugal Force

    Refrigerated Centrifuge Sigma 8KS 2 Layout and mode of operation → 2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.

  • Page 20: Safety

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Safety → Marking of the unit The following symbols are used on this centrifuge: Arrow indicating the direction of On (Power) rotation Rotor loading information Off (Power) (see chapter 6.2.2.4 - "Installation of accessories")

  • Page 21: Explanation Of The Symbols And Notes

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Explanation of the symbols and notes In this operating manual, the following names and symbols to indicate hazards are used: This symbol stands for a direct hazard to the life and health of persons.

  • Page 22: Responsibility Of The Operator

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Responsibility of the operator The operator is responsible for authorising only qualified personnel to work on the centrifuge (see chapter 3.4 - "Requirements concerning the personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.

  • Page 23: Requirements Concerning The Personnel

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Requirements concerning the personnel Risk of injury if the personnel are not sufficiently qualified If unqualified personnel perform work on the centrifuge or are present in the danger zone of the centrifuge, hazards result that can cause serious injuries and considerable damage to property.

  • Page 24: Informal Safety Instructions

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Informal safety instructions • This operating manual is a part of the product. • The operating manual must be kept at the location of use of the centrifuge. Ensure that it is accessible at all times.

  • Page 25: Safety Instructions

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Safety instructions 3.6.1 Electrical safety As protection against electric shock, the centrifuge is equipped with an earthed mains power cable and connector. To ensure the effectiveness of this safety feature, the following must be ensured: •...

  • Page 26: Chapter 7.3 - "Service Contact")

    Refrigerated Centrifuge Sigma 8KS 3 Safety • Do not spin any substances that could damage the material of the rotors and buckets of the centrifuge in any way. Highly corrosive substances, for example, damage the material and affect the mechanical strength of the rotors and buckets.

  • Page 27: Safety Instructions For Centrifugation

    Refrigerated Centrifuge Sigma 8KS 3 Safety → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection"). • Keep a safety range of at least 30 cm free around the centrifuge as well as with regard to walls and other devices.

  • Page 28: Rotors And Accessories

    Refrigerated Centrifuge Sigma 8KS 3 Safety → 3.6.7 Rotors and accessories → 3.6.7.1 Lifting and carrying rotors All swing-out rotors applicable for this centrifuge and the angle rotor 12510 weigh more than 18 kg. • Always lift the rotors with a lifting device or with a sufficient number of people helping you.

  • Page 29: Safety Of Rotors And Accessories

    Refrigerated Centrifuge Sigma 8KS 3 Safety 3.6.8 Safety of rotors and accessories 3.6.8.1 Marking Batch number and serial number During production, every rotor and bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection.
  • Page 30 Refrigerated Centrifuge Sigma 8KS 3 Safety Set number and bucket number Buckets are always produced in sets for a specific rotor. The set number is engraved on the bucket. In addition, every bucket is assigned a fixed position in the rotor by way of a bucket number.

  • Page 31: Safety Devices

    3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact"). → 3.7.5 Imbalance monitoring system A dialog box may pop up or emit a sound signal in order to indicate that the centrifuge is in the inadmissible imbalance range.

  • Page 32: Measures In The Event Of Hazards And Accidents

    Refrigerated Centrifuge Sigma 8KS 3 Safety → Measures in the event of hazards and accidents • If an emergency arises, switch off the centrifuge immediately! • If in doubt, call the emergency doctor! DANGER → Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules.

  • Page 33: Storage And Transport

    Refrigerated Centrifuge Sigma 8KS 4 Storage and transport → Storage and transport → Storage conditions The centrifuge can be stored in its original packaging for up to a year. • Store the centrifuge only in dry rooms. • The permissible storage temperature is between -20°C and +60°C.

  • Page 34: Set-Up And Connection

    Refrigerated Centrifuge Sigma 8KS 5 Set-up and connection → Set-up and connection The centrifuge must be set up and connected by authorised specialised personnel. All the relevant information is documented in a separate transport and installation manual. • Comply with the information given in the separate transport and installation manual of the centrifuge! •...

  • Page 35: Cover Holder

    Refrigerated Centrifuge Sigma 8KS 5 Set-up and connection Cover holder The cover of the swing-out rotor 11805 with a windshield can be placed in the lid of the centrifuge when loading or unloading the centrifuge. In addition, it is possible to install a cover holder on the right side of the centrifuge.

  • Page 36: Using The Centrifuge

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → Using the centrifuge → Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection"). DANGER →...

  • Page 37: Installation Of Rotors And Accessories

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.2.2 Installation of rotors and accessories All swing-out rotors applicable for this centrifuge and the angle rotor 12510 weigh more than 18 kg. • Always lift the rotors with a lifting device or with a sufficient number of people helping you.
  • Page 38 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Hexagon key Open-ended spanner Fig. 14: "Two-finger-principle" for holding the hexagon key (example) • Release the hexagon key, hold the rotor with one hand and tighten it clockwise by way of the open-ended spanner (see the following illustration) and with a torque of 20 Nm.
  • Page 39 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → Installation of a rotor 11805 with a windshield • Open the centrifuge lid by pressing the lid button. • Have two people lift the rotor (without its windshield cover) by its rotor arms (see the following picture).

  • Page 40: Installation Of An Angle Rotor With A Hermetic Lid

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge • Check for correct fit. ✓  Fig. 18: Checking the cover for correct fit • Comply with the safety and hazard notes (see chapter 3 - "Safety")! → 6.2.2.2 Installation of an angle rotor with a hermetic lid •...

  • Page 41: Rotor Removal

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge The rotor can also be used without a lid. NOTE → 6.2.2.3 Rotor removal View the video showing the rotor installation and removal process: NOTE The rotor removal must be performed in the reverse order of its installation.

  • Page 42: Installation Of Accessories

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.2.2.4 Installation of accessories • Only use vessels that are suitable for the rotor. • In the case of swing-out rotors, all of the compartments must be equipped with buckets. •...

  • Page 43: Adapters

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Pay attention to the marking of the centrifuge (see the illustration below)! Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced. NOTE Rotor: Bucket: Fig.

  • Page 44: Blood Bag Systems

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Maximum speed for tubes Some tubes, such as centrifuge glass tubes, microtubes, culture tubes, fluoropolymer tubes and especially high-volume tubes can be used in our rotors, buckets, and adapters at higher speeds than their breaking limit.
  • Page 45 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Proper loading Possible loading Improper loading Fig. 22: Loading of blood bag systems The service life of the adapter for blood bags no. 13867 is limited. Refer to the table of rotors and accessories with a different service life (see chapter 11.4 - "...

  • Page 46: Control System "Spincontrol S

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → Control system "Spincontrol S" → 6.3.1 User interface The centrifuge is operated via three buttons with integrated light-emitting diodes and one function knob. The display is divided into several different fields. The various functions of the system can be called up by pressing and turning the function knob.

  • Page 47: Manual Mode

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2 Manual mode → 6.3.2.1 Starting a centrifugation run The centrifuge is ready for operation when the start key is illuminated. • Press the start key in order to start a centrifugation run.

  • Page 48: Standard Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2.5 Standard menu The "Standard" menu is symbolised by the icon " " on the menu bar and it is displayed a few seconds after the centrifuge has been switched on. In this menu, the parameters of a centrifugation run can be displayed and modified.
  • Page 49 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Runtime The set runtime is displayed in the upper section of this field, with the remaining runtime shown below. The runtime is defined as the period from the start of the centrifuge to the beginning of the deceleration phase. The maximum value is 99 h 59 min 59 sec.
  • Page 50 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Rotor: rotor selection list This field shows the rotor that is currently being used. • Select the field "Rotor" and confirm the selection. A list with all of the possible rotors without buckets is displayed.
  • Page 51 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Acceleration This function is used to select an acceleration curve. One can select a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The acceleration curves 20-29 can be programmed as desired (see chapter 11.3 - "Acceleration and deceleration curves").
  • Page 52 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Fig. 28: Program "RAPID_TEMP" The program will only be loaded if the actual temperature is above the set temperature. NOTE • Press the start button in order to start the rapid cooling run.
  • Page 53 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge If the "RAPID_TEMP" program is used, the temperature of the unloaded aluminium bucket will be displayed. If samples, which have not been precooled, are placed into the buckets, the displayed temperature will deviate from the actual sample temperature.

  • Page 54: Process Library Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge 6.3.2.6 Process library menu The process library can be opened via the " " symbol on the menu bar. It provides the user with an overview of all of the stored programs as processes.

  • Page 55: Parameters Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2.7 Parameters menu The "Parameters" menu is symbolised by the " " symbol on the menu bar. It is used to specify various conditions for the centrifugation. These conditions are used to monitor the process and to control access to the centrifuge.
  • Page 56 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Density This setting is useful for glass vessels. If the density of the liquid to be centrifuged is higher than 1.2 g/cm , the value must be adapted manually in order to prevent the glass vessel from breaking. This will reduce the maximum possible final speed (see chapter 2.2.2.2 - "Density").
  • Page 57 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Temperature monitoring The control system includes a temperature monitoring function. If the actual temperature difference with regard to the set value deviates from the set temperature difference, the centrifuge will stop and an error message will be issued.
  • Page 58 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Lock In order to prevent any unauthorised use of the centrifuge, the following functions can be blocked: • Saving of programs (level 01) • Changing of parameters, short run (level 02) •...

  • Page 59: Setup Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2.8 Setup menu The "Setup" menu is symbolised by the " " symbol on the menu bar. It is used to perform basic settings concerning the control system of the centrifuge. It enables the optimum adaptation of the centrifuge to its specific area of application.
  • Page 60 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed. The symbol "" will be displayed in the runtime field. Display deceleration time In the activated mode, the deceleration time will be displayed instead of the runtime during and after the deceleration process.

  • Page 61: Chapter 6.3.4 - "Options For Data Input And Output")

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are used. Info This item provides information on the software versions that are used in this centrifuge.

  • Page 62: Curve Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2.9 Curve menu This menu is used to create and edit customised acceleration and deceleration curves (see chapter 11.3 - "Acceleration and deceleration curves"). It is symbolised by the " " symbol on the menu bar.

  • Page 63: 6.3.2.10 Option: Barcode Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Restrictions • Acceleration and deceleration curves can include intervals with a positive slope as well as intervals with a negative slope and also intervals with 0 slope. • The slope of the curve intervals can be 1 min...
  • Page 64 Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge With the barcode scanner, the barcodes can be scanned directly at the centrifuge during the loading process. The centrifugation data can be enquired cyclically (automatically) by the PC. Once a centrifugation run is complete, the PC can detect this and save the result (centrifugation data with barcodes).

  • Page 65: 6.3.2.11 Help Menu

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Deleting the stored barcodes: "deletebarcodes" This command is used to delete all of the stored barcodes. The "Barcode" menu will be updated. This command will not be accepted unless the centrifuge is at a standstill.

  • Page 66: 6.3.2.12 Changing The Contrast

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.2.12 Changing the contrast To change the contrast: • Press and hold the stop button and turn the function knob one notch to the left. A dialog box will be displayed once the stop button is released.

  • Page 67: Saving A Program

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.3.1 Saving a program • Enter the parameters that are to be included in the program. • Select the option "Progr" in the "Standard" menu and confirm the selection. The program list will be displayed.

  • Page 68: Executing A Program

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge Fig. 40: Loading a program → 6.3.3.3 Executing a program • Select the option "Progr" from the "Standard" menu and confirm the selection by pressing the function knob. The program list will be displayed.

  • Page 69: Automatic Program Rotation

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.3.5 Automatic program rotation With the automatic program rotation, several programs can be executed directly one after the other. • Activate the "Program rotation" function in the "Setup" menu Fig. 42: Program rotation function When a program is loaded while the program rotation function is active, this program will be used as the start program for the rotation.

  • Page 70: Options For Data Input And Output

    Refrigerated Centrifuge Sigma 8KS 6 Using the centrifuge → 6.3.4 Options for data input and output • Serial interface (see chapter 6.3.5 - "Connection of a separate computer") • External signal active DC 24 V, 0.5 A max. (part no. 17701) •...

  • Page 71: Malfunctions And Error Correction

    Refrigerated Centrifuge Sigma 8KS 7 Malfunctions and error correction → Malfunctions and error correction → General malfunctions Malfunctions are indicated by a dialog box. If the acoustic signal is activated, it sounds when the error message is displayed. • Eliminate the source of the problem (see table below).

  • Page 72: Emergency Lid Release

    Refrigerated Centrifuge Sigma 8KS 7 Malfunctions and error correction → 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.

  • Page 73: Table Of Error Codes

    Refrigerated Centrifuge Sigma 8KS 7 Malfunctions and error correction → Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to • Power off/on decelerate brakeless •...

  • Page 74: Service Contact

    Refrigerated Centrifuge Sigma 8KS 7 Malfunctions and error correction → Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-44 44 E-mail: support.lab@sigma-zentrifugen.de...

  • Page 75: Maintenance And Service

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure. If corrosion or other damage occurs due to improper care, the manufacturer cannot be held liable or subject to any warranty claims.

  • Page 76: Condenser (Only Refrigerated Centrifuges With An Air-Cooled Refrigeration System)

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → 8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system) In order to cool the refrigerant that is compressed by the refrigeration unit, centrifuges with an air-cooled refrigeration system use a lamellar condenser.

  • Page 77: Plastic Accessories

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → 8.1.2.1 Plastic accessories The chemical resistance of plastic decreases with rising temperatures (see chapter 11.5 - "Resistance data"). • If solvents, acids, or alkaline solutions have been used, clean the plastic accessories thoroughly.

  • Page 78: Load Bearing Bolts

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → 8.1.4 Load bearing bolts Only greased load-bearing bolts ensure a uniform swing-out of the buckets and, therefore, the smooth operation of the centrifuge. Load-bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance.

  • Page 79: Glass Breakage

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → 8.1.5 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles.

  • Page 80: Autoclaving

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.

  • Page 81: Service

    DIN EN 61010-1). The specified test in accordance with DGUV V3, DIN EN 61010-1 must be performed for centrifuges made by Sigma. The test is considered as passed if the specified limits are complied with. A measurement in accordance with VDE 0701-0702 leads to higher values, which is due to a different measurement method.
  • Page 82 Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service Outside Germany: Contact our agency in your country. All agencies are listed at www.sigma-zentrifugen.de → [Sales Partners] • If you would like to utilise our service, please state the type of your centrifuge and its serial number.

  • Page 83: Return Of Defective Centrifuges Or Parts

    Refrigerated Centrifuge Sigma 8KS 8 Maintenance and service → Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer. In order to ensure the quick and economical processing of returns of centrifuges, spare parts, or accessories, we require complete and extensive information concerning the process.
  • Page 84 NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 84 / 129 Original operating manual, part no. 0702502...

  • Page 85: Disposal

    Disposal → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...

  • Page 86: 10 Technical Data

    Refrigerated Centrifuge Sigma 8KS 10 Technical data → 10 Technical data → Manufacturer Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: Sigma 8KS Sigma 8KS, Clinic Order number: 10630, 10631, 10632, 91594 10633, 91300, 91301, 91367, 91583, 190010 Performance data Max.

  • Page 87: Ambient Conditions

    Refrigerated Centrifuge Sigma 8KS 10 Technical data → 10.1 Ambient conditions • The figures are valid for an ambient temperature of +23°C and a nominal voltage  10 %. The minimum temperature is ≤ +4°C and depends on the rotor type, speed, and ambient temperature.

  • Page 88: 11 Appendix

    Refrigerated Centrifuge Sigma 8KS 11 Appendix → 11 Appendix → 11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below. The radius calculation is described in chapter 2.2.2.1 - "Speed, radius, and relative centrifugal...

  • Page 89: Speed-Gravitational-Field-Diagram

    Refrigerated Centrifuge Sigma 8KS 11 Appendix → 11.2 Speed-gravitational-field-diagram Fig. 49: Speed-gravitational-field-diagram Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 89 / 129 Original operating manual, part no. 0702502...

  • Page 90: Acceleration And Deceleration Curves

    Refrigerated Centrifuge Sigma 8KS 11 Appendix → 11.3 Acceleration and deceleration curves Linear as well as quadratic curves are numbered in the direction of increasing acceleration (from right to left). The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers.
  • Page 91 Refrigerated Centrifuge Sigma 8KS 11 Appendix Fig. 52: Diagram of linear curves Fig. 53: Diagram of quadratic curves Version 08/2012, Rev. 2.14 - 2025-03-05 • sb 91 / 129 Original operating manual, part no. 0702502...

  • Page 92: Table Of The Service Life Of Rotors And Accessories

    Refrigerated Centrifuge Sigma 8KS 11 Appendix → 11.4 Table of the service life of rotors and accessories • If no other data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10 years.

  • Page 93: Resistance Data

    Refrigerated Centrifuge Sigma 8KS 11 Appendix → 11.5 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol...
  • Page 94 Refrigerated Centrifuge Sigma 8KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H Cyclohexanol Decane Diaminoethane —...
  • Page 95 Refrigerated Centrifuge Sigma 8KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol Methyl benzene Methyl ethyl ketone...
  • Page 96 Refrigerated Centrifuge Sigma 8KS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate Spirits Styrene...

  • Page 97: Serial Control Interface Specification

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  • Page 121: Ec Declaration Of Conformity

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  • Page 123: Declaration Of Conformity - China Rohs 2

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  • Page 125: 12 Index

    Refrigerated Centrifuge Sigma 8KS Index 12 Index Acceleration ..........51 Capacity ............. 86 Acceleration curve ......51, 62, 90 Carrier ............43 Acceleration curve (changing) ....62 Carrier systems .......... 43 Acceleration curve (creating) ..... 62 Carriers, cleaning and care ......77...
  • Page 126 Refrigerated Centrifuge Sigma 8KS Index Continuous heat resistance ......79 Continuous run .......... 49 Earth conductor check ....... 31 Contrast (changing) ........66 EC declaration of conformity ....11, 121 Control system ........... 46 EEPROM error ........... 73 Cooling water connection ......15 Electrical connection ........
  • Page 127 Refrigerated Centrifuge Sigma 8KS Index Importance of the operating manual ..... 9 Manufacturer ..........86 Important information ......... 21 Marking of rotors and accessories ..... 29 Improper loading ........71 Marking of the unit ........20 Infectious substances ........ 79 Maximum speed for tubes ......
  • Page 128 Refrigerated Centrifuge Sigma 8KS Index Power cord is not plugged in ...... 71 Rotor tie-down screw ......... 77 Pressure (refrigerant) ......... 86 Rotors Pressure marks .......... 77 marking ........... 29 Prevention of accidents ........ 9 Rotors and accessories with a different service life ........
  • Page 129 Refrigerated Centrifuge Sigma 8KS Index Speed/RCF fine ......... 60 Temperature ..........48 Speed-gravitational-field-diagram ....89 Temperature at water inlet ......86 Speedometer error ........73 Temperature error ........73 Spin-out from set speed ......57 Temperature inside the rotor chamber ..31 Standard menu ..........

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