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740412.10
Macherey-Nagel 740412.10 Manuals
Manuals and User Guides for Macherey-Nagel 740412.10. We have
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Macherey-Nagel 740412.10 manual available for free PDF download: User Manual
Macherey-Nagel 740412.10 User Manual (69 pages)
Plasmid DNA Purification
Brand:
Macherey-Nagel
| Category:
Laboratory Equipment
| Size: 1.19 MB
Table of Contents
Table of Contents
3
1 Components
5
Kit Contents
5
Reagents and Equipment to be Supplied by User
7
2 Kit Specifications
8
3 About this User Manual
9
4 Nucleobond
10
Basic Principle
10
Growth of Bacterial Cultures
12
Culture Volume for High-Copy Plasmids
13
Culture Volume for Low-Copy Plasmids
14
Cell Lysis
15
Difficult-To-Lyse Strains
15
Setup of Nucleobond
16
Filtration and Loading of the Lysate
17
Washing of the Column
17
Elution and Concentration of Plasmid DNA
18
Determination of DNA Yield and Quality
21
Convenient Stopping Points
21
5 Storage Conditions and Preparation of Working Solutions
22
6 Safety Instructions - Risk and Safety Phrases
23
Nucleobond
24
High-Copy Plasmid Purification (MIDI, Maxi)
24
Low-Copy Plasmid Purification (MIDI, Maxi)
29
7 Concentration of Nucleobond Nucleobond
32
Concentration of Nucleobond
32
Xtra Eluates with the ® Finalizers - French
32
High-Copy Plasmid Purification (MIDI, Maxi)
34
Low-Copy Plasmid Purification (MIDI, Maxi)
40
Concentration of Nucleobond Nucleobond
44
High-Copy Plasmid Purification (MIDI, Maxi) - French
47
Low-Copy Plasmid Purification (MIDI, Maxi) - French
53
8 Appendix
60
Troubleshooting
60
Ordering Information
68
Product Use Restriction / Warranty
68
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Macherey-Nagel Categories
Laboratory Equipment
Measuring Instruments
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