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Section 7
7.1 Setting Up Single Wavelength Mode
7.2 Taking Single Wavelength Measurements
5940018SingleWave.fm
Single Wavelength Mode
The Single Wavelength Mode can be used in three ways. For a known parameter
wavelength, set the instrument to measure the absorbance, % transmittance, or
concentration of the analyte.
Percent (%) transmittance measures the percent of the original light that passes
through the sample and reaches the detector.
Absorbance measures the amount of light absorbed by the sample, in units of
Absorbance.
Turning on the concentration factor allows selection of a specific multiplier for
converting absorbance readings to concentration. In a graph of concentration
versus the absorbance, the concentration factor is the slope of the line.
1. From the Main Menu, touch
2. Look at the second button from the top on the vertical menu bar.
Touch
%T
switch to absorbance readings. Touch
concentration, if it is enabled (see step 5).
3. Touch λ on the right menu bar to change the wavelength. Key in
the desired wavelength and touch
4. Touch
Options
concentration mode, and set the desired resolution for
concentration readings.
5. To convert absorbance readings to concentration, touch
Concentration Factor
to enter the factor by which absorbance readings are to be
Factor
multiplied. Touch
measurements. Touch
6. To change the number of decimal places shown in the
Concentration reading, touch
make a selection.
In Single Wavelength reading mode, the instrument will begin reporting a
concentration value. Touch
reading modes.
1. Prepare a blank.
2. Place the sample cell containing the blank in the cell holder. Touch
3. Remove the blank from the cell compartment. Place the sample cell in the cell
holder. Read the results. (The reading will appear within 20 seconds.)
Single Wavelength
to switch to % transmittance readings. Touch
to access store, send, or recall data, turn on the
. Touch
to turn on this feature. Touch
On
to select the units for concentration
Units
.
OK
Concentration Resolution
to toggle through the various
Abs/%T
.
Abs
to switch to
Conc
OK.
and
Zero
Single Wavelength Mode
to
.
Page 47
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