PerkinElmer Migele User Manual
  1. Manuals
  2. Brands
  3. PerkinElmer Manuals
  4. Laboratory Equipment
  5. Migele
  6. User manual

PerkinElmer Migele User Manual

Gel electrophoresis unit
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
®
Migele
Gel Electrophoresis Unit
2118-0010 User Manual

Advertisement

Table of Contents
loading

Related Manuals for PerkinElmer Migele

Summary of Contents for PerkinElmer Migele

  • Page 1 ® Migele Gel Electrophoresis Unit 2118-0010 User Manual...

  • Page 3: Table Of Contents

    Cooler setting................................21 Power setting................................21 Electrode setting................................21 Placing gel..................................22 Closing the unit................................22 Starting the run................................23 Ending the run................................23 IQ/OQ for Migele Gel Electrophoresis Unit..........25 Maintenance.....................26 Daily maintenance................................26 Cleaning the cooling plate..........................26 Lid..................................26 Electrodes................................ 27 Monthly maintenance..............................27...
  • Page 4 Decontamination information............................. 28 Technical specifications................29 Troubleshooting..................30 Contact addresses..................32 WEEE Instructions for PerkinElmer Products.......... 33 Warranty....................34 Index......................35...

  • Page 5: General Information

    (IEF) gels. The Migele Gel Electrophoresis Unit is intended to be used by trained laboratory personnel.
  • Page 6 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual General information Symbol Description Caution, consult instructions for use (Label) (Instrument) Consult instructions for use In vitro diagnostic medical device Catalogue number Serial number This way up Fragile, handle with care...

  • Page 7: Safety Information

    Warning: This instrument requires high voltage in operation. Warning: Do not attach the Migele Gel Electrophoresis Unit to the portable power supply if you identify any damage on either of them. Also monitor wires. If you identify any damage, do not use the wires.
  • Page 8 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Safety information it to the manufacturer and to your national authority. The contact information for the manufacturer of this device to report a serious incident is: Wallac Oy Mustionkatu 6, FI-20750 Turku, Finland http://www.perkinelmer.com...

  • Page 9: Contents Of The Package

    Quality control certificate • Installation kit (61012727) Materials required but not provided with this package The following items are required and are available from Wallac Oy or PerkinElmer, Inc. and its distributors: • Programmable Power Supply (CWP-2000) • Circulating Water Bath (1023-0010, 1023-0020) •...

  • Page 10: Instrument Overview

    Instrument overview Instrument overview The Migele Gel Electrophoresis Unit is used together with the programmable power supply (CWP-2000 or equivalent) and the circulating water bath (1023-0010, 1023-0020) or equivalent. The instrument can run gels of different sizes. For example, 240 × 203 mm (FR-9400, FR-9360) and 203 × 127 mm (FR-9120).
  • Page 11 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Instrument overview Figure 2: Migele Gel Electrophoresis Unit connections Water tubing is shown on the right, and the power supply connections are shown on the left part of the instrument.

  • Page 12: Description Of Parts

    Description of parts Description of parts Two to four 2118-0010 Migele Gel Electrophoresis Units can be attached to series with one circulating water bath (for example, 1023-0010, 1023-0020 or equivalent). Two to four 2118-0010 Migele Gel Electrophoresis Units can be attached to one programmable power supply (e.g.

  • Page 13: Lid With Electrodes

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Description of parts Lid with electrodes The lid contains a fixed cathode electrode in the middle and two anodes at both sides of the cathode. Anode positions can be modified and positioned correctly in accordance with the grid and gel requirements.
  • Page 14 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Description of parts Figure 5: Electrodes seen through top window. Cathode positioned in the middle and two anodes at the both sides of the cathode. The two anode electrodes can be adjusted by clamping nuts located at each end of the electrode. The distance from the...

  • Page 15: Installation

    (not included). Use the same kit and gel that you are planning to use for the samples. As the Migele Gel Electrophoresis Unit is intended to control the gel processing condition, avoid warm exhaust air from other instruments when placing the instrument. Leave sufficient space behind the instrument for tubing and ventilation.
  • Page 16 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Installation Figure 7: Removing transport protection Figure 8: Removing protective plastic sheet...

  • Page 17: Connecting The Cooling System (Single Unit)

    Gel Electrophoresis Unit User Manual Installation Connecting the cooling system (single unit) The Migele Gel Electrophoresis Unit contains connectors on both inlet and outlet tubing (male and female) (see Figure 9: Connecting tubing on page 13). The package contains tubing with the corresponding connectors to be used with the circulating water bath.

  • Page 18: Levelling The Instrument

    Note: Using HI setting in the circulator pump speed is recommended. Levelling the instrument When the Migele Gel Electrophoresis Unit is in the final bench location, place a spirit level on the cooling plate (see Figure 11: Spirit level to monitor that the instrument is levelled correctly...
  • Page 19 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Installation Figure 11: Spirit level to monitor that the instrument is levelled correctly...
  • Page 20 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Installation Figure 12: Adjust the unit with the levelling feet Use 10 mm wrench to tighten the nut to lock the foot position. You can turn the device on the side to get better access to loosen the locking nuts. Adjust first the back feet to get the device on level sideways.

  • Page 21: Setting The Lid

    Connecting the power supply Warning: The supplied DC cables are rated for 5000 V. Only use cables and adapters supplied with the Migele Gel Electrophoresis Unit or ensure that they have a suitable DC insulation compliance for the used voltages.

  • Page 22: Connecting The Cooling System (Multiple Units)

    Figure 13: Connect the power supply and the Migele Gel Electrophoresis Unit Connect the power supply and the Migele Gel Electrophoresis Unit with the red and black wires according to the pictures. EPS 3501 XL requires 4/2 mm adapters in the power supply end.
  • Page 23 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Installation Figure 14: Connecting the units...
  • Page 24 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Installation Figure 15: Stacked GEUs...

  • Page 25: Operation

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Operation Operation Wear suitable protective clothing, gloves and eye protection. Disposal of all waste should be in accordance with local regulations. Cooler setting Turn the circulating water bath on and set the desired temperature according to the kit insert. Allow time for the water to reach the desired temperature.

  • Page 26: Placing Gel

    Placing gel Pull the tray to the outward position. Ensure that the Migele Gel Electrophoresis Unit's cooling plate is clean. Follow the kit insert instructions when positioning the gel to the cooling plate. You can use the grid to position the gel. Cathode wick needs to be positioned in the 0 position.

  • Page 27: Starting The Run

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Operation Figure 19: Idle position without green spot visible Starting the run Start the run by turning on the power supply. You can monitor the progress from the window (see Figure 20: Top window to monitor the run on page 23).
  • Page 28 24). After runs ensure that the tubing and wires are in good condition and not worn out (if the instrument is old). If you have any questions, please contact your local PerkinElmer representative. Figure 21: Lid in idle position...

  • Page 29: Iq/Oq For Migele Gel Electrophoresis Unit

    Compare results with picture below. Gel bands should be straight and positioned similarly over the cooling plate. If there is anything that is not positioned correctly, please take a picture of the gel and send it to your local PerkinElmer representative to be investigated.

  • Page 30: Maintenance

    Warning: Disconnect the external power supply before cleaning or servicing. Daily maintenance This section includes instructions for the daily maintenance of the Migele Gel Electrophoresis Unit. Cleaning the cooling plate Wipe the cooling plate with watered paper towel (lint-free paper towel) and dry it. It is recommended to clean the cooling plate immediately after the run, and if required, also before the run.

  • Page 31: Electrodes

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Maintenance Electrodes Electrodes should be wiped with watered paper lint-free towel once a day and also in between the runs to prevent the crystallisation of buffer salts, which can affect the run performance if not cleaned. We recommend you to monitor the electrodes and change the lid if the electrodes are worn out.

  • Page 32: Decontamination Information

    Press it against the cooling plate surface and tighten the hex screws. Decontamination information The following instructions should be followed before performing any service or disassembly with the Migele Gel Electrophoresis Unit. Note: Use gloves when performing any service with parts that may have been into contact with patient samples (blood spots).

  • Page 33: Technical Specifications

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Technical specifications Technical specifications Cooling plate maximum pressure: 0.5 bar Coolant temperature range: +10 - +15 °C Maximum voltage: 2000 V Maximum power: 100 W Dimensions: Width 351 mm, Length 520 mm, Height with levelling feet 125-135 mm Weight: approx.

  • Page 34: Troubleshooting

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Troubleshooting Troubleshooting Problem Cause Action Gel separated from backing during Too much pressure applied to the Use a light touch when blotting the blotting blotting paper gel. Blotting paper rested on the gel too...
  • Page 35 ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Troubleshooting Problem Cause Action Excess condensation during the run Humidity can affect the running Open the lid and blot the gel during conditions the run. Additional blotting can also be performed.

  • Page 36: Contact Addresses

    ® 2118-0010 - Migele Gel Electrophoresis Unit User Manual Contact addresses Contact addresses Manufacturer Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland Tel: 358-2-2678111. Fax: 358-2-2678 357. Email: info@perkinelmer.com Website: www.perkinelmer.com Service Please contact your local PerkinElmer representative...

  • Page 37: Weee Instructions For Perkinelmer Products

    Customer Care Rest of the World: call (+1) 203-925-4602 Products from other manufacturers may also form a part of your PerkinElmer system. These other producers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive.

  • Page 38: Warranty

    Warranty Warranty The enclosed instrument comes with a limited warranty (the “Warranty”) from Wallac Oy (“Wallac”), a PerkinElmer company. This is only a summary of the warranty on the enclosed instrument and shall not be deemed to alter or amend the terms of the Warranty. Consult the terms and conditions, and rental or purchase agreement for a full explanation of the Warranty.

  • Page 39: Index

    Electrical safety Environmental conditions Installation Connecting cooling system Multiple GEUs Single GEU Connecting power supply Levelling instrument Setting the lid Stacking several GEUs Instrument Migele GEU Power Supply Circulating Water Bath Intended purpose IQ/OQ Maintenance Daily Monthly Operation Closing unit Cooler...
  • Page 42 2118-9010-03 April 28, 2022 Manufacturer: Wallac Oy Mustionkatu 6, FI-20750 Turku, Finland. Tel: 358-2-2678 111. Fax: 358-2-2678 357 www.perkinelmer.com...

Table of Contents