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PerkinElmer Raman Micro 200 Series Getting Started Manual

PerkinElmer Raman Micro 200 Series Getting Started Manual

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MOLECULAR SPECTROSCOPY
RamanMicro 200 Series
Getting Started Guide

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  • Page 1 MOLECULAR SPECTROSCOPY RamanMicro 200 Series Getting Started Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    RamanMicro 200 Series Getting Started Guide . 3 Table of Contents Introduction ....................5 About This Manual ..................6 Conventions Used in this Manual ..............7 Notes, Cautions and Warnings ..............7 Safety Practices ..................11 Overview ....................12 General Operating Conditions ................ 13 Environmental Conditions ................
  • Page 4 Maintenance and Service of the Microscope ..........106 Maintenance and Service of the Fiber Optic Probe ........106 Replacing the Stage Controller Fuse .............. 107 Troubleshooting ..................108 Hardware .................... 108 Software ..................... 111 Decontamination ..................113 WEEE Instructions for PerkinElmer Products ..........114...

  • Page 5: Introduction

    Introduction...

  • Page 6: About This Manual

    6 . RamanMicro 200 Series Getting Started Guide About This Manual This Getting Started Guide contains all of the information that you will need to setup your RamanMicro 200 Series Raman Microscope and start collecting spectra. The Getting Started Guide is divided into the following chapters: Introduction This chapter contains an overview of the manual, and the conventions and warnings used in the manual.

  • Page 7: Conventions Used In This Manual

    ALT+F. All eight digit numbers are PerkinElmer part numbers unless stated otherwise. The term RamanMicro 200 Series means the RamanMicro 200 and the RamanMicro 200F Raman Microscopes.
  • Page 8 8 . RamanMicro 200 Series Getting Started Guide We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one. Caution (Achtung) Bedeutet, daß...
  • Page 9 Introduction . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann.
  • Page 10 10 . RamanMicro 200 Series Getting Started Guide...

  • Page 11: Safety Practices

    Safety Practices...

  • Page 12: Overview

    All information provided herein is for reference only. All information is believed to be correct and complete. PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document. PerkinElmer does not accept liability for the interpretation of information resulting, either directly or indirectly, from our spectrometers or associated software interfaces.

  • Page 13: General Operating Conditions

    General Operating Conditions The RamanMicro 200 and RamanMicro 200F spectrometers have been designed and tested in accordance with PerkinElmer specifications and in accordance with the safety requirements of the International Electrotechnical Commission (IEC). The RamanMicro 200 Series conforms to IEC 61010-1 (Safety Requirements for electrical equipment for measurement, control and laboratory use) as it applies to IEC Class 1 (earthed) appliances and therefore meets the requirements of EC directive 2006/95/EC.

  • Page 14: Environmental Conditions

    14 . RamanMicro 200 Series Getting Started Guide Environmental Conditions The instrument has been designed to be safe under the following conditions: • Indoor use; • Altitude up to 2000 m; • Ambient temperatures of 5 °C to 40 °C; A maximum ambient relative humidity of 80% for temperatures up to 31 °C, decreasing •...

  • Page 15: Fire Safety

    Safety Practices . 15 Fire Safety Ensure that there is at least 15 cm (6”) between any cooling vents and adjacent • equipment or walls. • Ensure that the power supply unit (PSU) vents or fan do not become obstructed (Figure 1).

  • Page 16: Electrical Safety

    100– 240 VAC ±10% of the nominal voltage Under no circumstances should you attempt to disassemble the PSU and carry out repairs. If the PSU has a fault, please contact your PerkinElmer Service Engineer. The entire unit must be replaced. WARNING Connect the PSU to a three-wire (live, neutral and ground/earth) socket.

  • Page 17: Power Cords (Between Psu And Power Outlet Socket)

    Safety Practices . 17 Power Cords (Between PSU and Power Outlet Socket) The power cord supplied to connect the PSU to the power point (socket) should be appropriate for the country listed as the shipping destination. If the power cord is not appropriate for your country it must be removed from use immediately and replaced.

  • Page 18: Emc Compliance

    18 . RamanMicro 200 Series Getting Started Guide EMC Compliance EC directive The RamanMicro 200 and RamanMicro 200F have been designed and tested to meet the requirements of the EC directive 2004/108/EC. The RamanMicro 200 Series complies with the EMC standard EN61326 (EMC standard for electrical equipment for measurement, control and laboratory use), and EN55011 (ISM) Class A (rf emissions).

  • Page 19: Laser Safety

    If the protective covers or service panels on the instrument appear to be damaged, turn the instrument off, secure it against any unauthorized WARNING operation and contact PerkinElmer for advice before using the instrument again. The RamanMicro 200 emits a focused beam of laser radiation at 785 nm (250 mW max.), and is classified as a Class 3B laser system.

  • Page 20: Class 3B Safety Precautions

    20 . RamanMicro 200 Series Getting Started Guide Class 3B Safety Precautions You should follow Class 3B laser safety precautions such as the use of door interlocks and the wearing of laser safety goggles. For information on these precautions refer to an appropriately trained laser safety officer within your own facility.
  • Page 21 Safety Practices . 21 Microscope Safety The laser should also be turned off when the system is not in use. AVOID DIRECT EXPOSURE TO THE BEAM. Class 3B laser radiation is emitted through the objective lens Figure 2 Schematic of microscope When using the microscope, the following precautions must always be observed: •...
  • Page 22 22 . RamanMicro 200 Series Getting Started Guide Do not make any adjustments to the microscope or any part of the system when experiments are in progress. In particular: WARNING Do not remove the objective lens from the microscope; • Do not insert or remove samples;...
  • Page 23 Safety Practices . 23 Fiber Optic Probe Safety NOTE: This section is only applicable to RamanMicro 200F systems equipped with a fiber optic probe. The fiber optic probe body incorporates a number of important safety features, including: Beam attenuator/cover; • Emission indicator/LED.
  • Page 24 PerkinElmer (part number L1323518). Do not use the TFOP if it shows any sign of damage. • If repair or servicing is needed, call your PerkinElmer Service Representative for advice. • Raman Triggered Fiber Optic Probe...
  • Page 25 Safety Practices . 25 Calculation of Nominal Hazard Zones for use of Fiber Optic Probe The following calculations show how to calculate a nominal hazard zone. These calculations are taken from ANSI Z136.1-1933. A laser safety officer uses these calculations to help define how to use your Raman probe safely in your environment.

  • Page 26: Internal Interlock System

    7.5 (± 10%) 27.3 NOTE: Other focal lengths are available; contact PerkinElmer for details. In particular, the use of an ultra-long working distance lens adaptor (part number L1320071) provides a working distance of 25 mm. Where different focal lengths are used, the NOHD should be calculated accordingly.

  • Page 27: Warning Labels

    Safety Practices . 27 Warning Labels When this label is attached to an instrument it means refer to the manual to find out the nature of the potential hazard and any actions which have to be taken. Labels on the Exterior of the Spectrometer LASER RADIATION LASER RADIATION AVOID EXPOSURE TO BEAM...

  • Page 28: Labels On The Exterior Of The Microscope

    28 . RamanMicro 200 Series Getting Started Guide Labels on the Exterior of the Microscope CAUTION – CLASS 3B LASER CAUTION – CLASS 3B LASER RADIATION WHEN OPEN RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM AVOID EXPOSURE TO BEAM LASER APERTURE Figure 6 Labels on the microscope...

  • Page 29: Raman History, Theory And Instrumentation

    Raman History, Theory and Instrumentation...

  • Page 30: The History Of Raman Spectroscopy

    30 . RamanMicro 200 Series Getting Started Guide The History of Raman Spectroscopy Inelastic light scattering was first predicted in 1923 by A. G. Smekal in Germany. Research carried out by A. H. Compton discovered inelastic scattering of X-rays, which led the Indian scientist C.

  • Page 31: Raman Theory

    Raman History, Theory and Instrumentation . 31 Raman Theory When photons from a monochromatic light source impinge on a sample, a small fraction are scattered in all directions. Some of the scattered photons are of the same energy as those of the incident beam, and hence the same frequency, giving rise to Rayleigh scattering (Figure 7).
  • Page 32 32 . RamanMicro 200 Series Getting Started Guide The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a change in the vibrational and/or rotational energy of the molecule. The frequency shifts from the frequency of the incident beam are displayed on the x-axis of a Raman spectrum, generally in relative wavenumbers (otherwise known as Raman Shift) and the intensity of the bands are displayed in arbitrary units on the y-axis.

  • Page 33: The Ramanmicro 200

    Raman History, Theory and Instrumentation . 33 The RamanMicro 200 The RamanMicro 200 is a fiber-optic Raman microscope with laser excitation wavelength at 785 nm. Alignment camera Raman module Illumination module Objectives Microscope frame Stage Figure 10 The RamanMicro 200 microscope The microscope (Figure 10) is composed of a frame, illumination module, Raman module and camera module.

  • Page 34: The Czerny-Turner Spectrograph

    34 . RamanMicro 200 Series Getting Started Guide The Czerny–Turner Spectrograph The RamanMicro 200 is equipped with a Czerny–Turner spectrograph. The Czerny–Turner spectrograph is the most commonly used spectrograph design in Raman spectroscopy. Here, light is focused into the spectrograph, collimated and shone on a grating which disperses that light in one dimension.

  • Page 35: Preparing Your Facility

    Preparing Your Facility...

  • Page 36: Specifications

    Class 3B – If the electricity supply does not conform to these specifications, please consult PerkinElmer prior to installation. If the supply of power is erratic please use an uninterruptible power supply (UPS). Incorrect shutdown, power fluctuations or brown-outs may damage the...

  • Page 37: Pc Requirements

    Preparing Your Facility . 37 PC Requirements The following pages provide details of the requirements for the PC that will run the Spectrum software and communicate with the instrument. To ensure successful installation of the software, please check these requirements before starting the installation. Hardware Requirements The PC on which you install the software must meet the following minimum specifications: ®...

  • Page 38: Windows Administrator Level

    38 . RamanMicro 200 Series Getting Started Guide Windows Administrator Level It is important to note that you must be logged on at Administrator level on Windows before installing the software. Logging on as an Administrator ensures that installation of the software can be undertaken and that the necessary system registry updates that form part of the installation process are successfully completed.

  • Page 39: Finding A Suitable Location For The Instrument

    Preparing Your Facility . 39 Finding a Suitable Location for the Instrument The following conditions should be observed: • The environment must be suitable for a Class 3B laser system. • If your system has a manual stage there must be a minimum of 4 power points (1 for the microscope, 1 for the spectrometer and 2 for the PC).

  • Page 40: Instrument Dimensions

    40 . RamanMicro 200 Series Getting Started Guide Instrument Dimensions The RamanMicro 200 spectrometer module weighs approximately 20 kg. The microscope module weighs approximately 20 kg. Laboratory benching must be strong enough to support the combined weight of the instrument and any ancillary equipment without warping or wobbling.

  • Page 41: Installing Your Ramanmicro 200

    Installing Your RamanMicro 200...

  • Page 42: Unpacking The Instrument

    When unpacked, check the RamanMicro 200 microscope system for any physical damage. If there is any damage, do not continue with the installation. Contact your PerkinElmer Service Representative immediately. Part Number...

  • Page 43: Lifting The Instrument

    Installing Your RamanMicro 200 . 43 Lifting the Instrument The RamanMicro 200 spectrometer and microscope modules both weigh 20 kg. To avoid injury or damage to the instrument, please use proper lifting techniques. To lift and/or move the instrument safely requires two people. WARNING Always hold the instrument under the base when you lift the spectrometer.

  • Page 44: Attaching The External Interlock Override

    (L1320264) supplied with the instrument. If your laser safety officer determines that an interlock is required on the door to the room, you will need to purchase the Raman Laser Safety Kit (L1320262). For more information contact your PerkinElmer representative.

  • Page 45: Assembly Of The Microscope

    (Figure 14). Fixing screw Figure 14 Fitting the stage 9. Replace the objectives. If you have a manual stage, you can upgrade to a motorized stage at any time. For more information contact your PerkinElmer representative.

  • Page 46: Connecting The Motorized Stage

    46 . RamanMicro 200 Series Getting Started Guide Connecting the Motorized Stage The motorized stage is powered by the stage controller box (L1860289) and joystick (L1865723). Figure 15 Stage controller and joystick To connect the motorized stage: 1. Connect the cable from the joystick to the socket labeled JOYSTICK on the rear panel of the stage controller box (Figure 16).
  • Page 47 Installing Your RamanMicro 200 . 47 Attaching the Z-axis Focus Motor and Sleeve NOTE: If you have purchased the optional microscope laser-safe enclosure, you may find it easier to attach the z-axis focus motor and sleeve before fitting the enclosure. If you install the enclosure first, you will need to pass the cable through the rear port of the enclosure.

  • Page 48: Attaching The Illumination Source

    48 . RamanMicro 200 Series Getting Started Guide Attaching the Illumination Source To attach the illumination source to the illuminator module: 1. Hold the illumination source in position against the rear of the illuminator module. 2. Using the allen key supplied (Figure 18), fasten the two screws so that the source is supported.

  • Page 49: Connecting The Raman Module To The Microscope

    Installing Your RamanMicro 200 . 49 Connecting the Raman Module to the Microscope The Raman module is positioned on top of the illuminator module so that the connecter bracket is aligned above the mounting hole. Ensure that the side of the Raman module is aligned with the illuminator module and then tighten the grub screw.

  • Page 50: Connecting The Microscope To The Spectrometer Module

    50 . RamanMicro 200 Series Getting Started Guide Connecting the Microscope to the Spectrometer Module The microscope is connected to the spectrometer module by two fiber optic cables. The excitation fiber transmits the laser energy from the spectrometer to the microscope, and the collection cable transmits the collected Raman data back to the detector.
  • Page 51 Installing Your RamanMicro 200 . 51 Make sure that the polarizing ridge on the fiber optic cable is aligned with the slot in the coupler casing before carefully pushing the cable into the fiber bracket. Coupler Polarizing ridge aligned with slot Fiber optic cable Figure 22 Connecting the fiber optic cable 2.

  • Page 52: Unpacking And Assembling The Pc

    1. Connect the mains power cords from the PC and the monitor into the mains electrical supply, and then switch on. 2. If you are prompted to login to Windows when you start the PC, log in. If your PC was provided by PerkinElmer your username and password will be: Username: Administrator Password: Administrator 3.

  • Page 53: Switching On The Instrument

    The RamanMicro 200 is supplied with two keys. One should be placed in the instrument and the other should be stored in a safe place. Replacement keys (L1323503) can be obtained from PerkinElmer. A laser safety officer or lab manager can control use of the instrument with these keys.

  • Page 54: Installing Spectrum Software

    54 . RamanMicro 200 Series Getting Started Guide Installing Spectrum Software Before you Start NOTE: We strongly suggest you read the PC Requirements starting on page 37 before attempting to install your software. NOTE: Before installing the software, we recommend that you read and print the Release Notes which can be found as a .rtf file and a .pdf file on the root of the CD, because they contain important information that may not be in this manual or the Spectrum Help.

  • Page 55: Installation Procedure

    Installing Your RamanMicro 200 . 55 Installation Procedure NOTE: It is important to note that you must be logged on at Administrator level on Windows before installing the software. The Installation Wizard helps you install the software on your PC. 1.
  • Page 56 56 . RamanMicro 200 Series Getting Started Guide 4. Click Next. The License Agreement page is displayed. 5. Read the license and if you accept the terms, select that option and then click Next. You will then be asked which products you want to install. 6.
  • Page 57 Installing Your RamanMicro 200 . 57 7. Click Next. The Ready to Install the Program page is displayed. 8. Click Install to begin installing Spectrum. The Installing Spectrum page is displayed, which informs you of the status of the installation. When the installation is complete, the InstallShield Wizard Completed page is displayed.
  • Page 58 58 . RamanMicro 200 Series Getting Started Guide 9. Click Finish. Before you can collect spectra you must configure your Raman instrument. Raman Instrument Installation Disk 10. Place your (LX108925) into your CD drive. 11. If the installation program does not start automatically, from the Start Menu select Run. The Run dialog is displayed.
  • Page 59 Installing Your RamanMicro 200 . 59 13. Click Next. The License Agreement page is displayed. 14. Read the license and if you accept the terms, select that option and then click Next. The Ready to Install the Program page is displayed.
  • Page 60 60 . RamanMicro 200 Series Getting Started Guide 15. Click Install to begin installing the Raman instrument. The Installing Raman Instrument page is briefly displayed. During this installation, the DirectX Setup page is displayed. 16. Read the license and if you accept the terms, click Yes. The Installing Raman Instrument page is displayed again, which informs you of the status of the installation.
  • Page 61 Installing Your RamanMicro 200 . 61 17. Click Next. The Raman Instrument Install Wizard opens at the Welcome page. NOTE: Ensure that the USB cable from your alignment camera (on the instrument and/or the microscope) is not connected into the USB socket in the sample compartment of the instrument.
  • Page 62 If you have already installed PerkinElmer software on the PC, the PerkinElmer Login dialog is displayed. Login as a PerkinElmer Software Administrator. Use the Administrator User name that you use for the PerkinElmer software that is already installed on the PC. The default User name and Password are administrator.
  • Page 63 NOTE: If you disconnect the instrument control USB cable and reattach it to a different USB port, then you will be prompted for the drivers. By default this driver is stored in C:\Program Files\PerkinElmer\ServiceIR\Raman. 24. Wait until the Welcome to the Found New Hardware Wizard is displayed.
  • Page 64 26. Select Install from a list or specific location (Advanced) and then click Next. The Please choose your search and installation options page is displayed. 27. Select Include this location in the search, click Browse, and then navigate to C:\Program Files\PerkinElmer\ServiceIR\Raman. 28. Click Next. The Completing the Found New Hardware Wizard is displayed.
  • Page 65 Installing Your RamanMicro 200 . 65 29. Click Finish. The Found New Hardware Wizard closes. 30. On the Install PerkinElmer Raman Instrument page, click Continue. The Instrument Installation Complete page is displayed. 31. Click Finish. The Raman Instrument Installer closes.
  • Page 66 36. If you have a Videology alignment camera, you will need to install the Videology USB2.0 Viewer. Go to Step 37. If not, go to Step 45. Navigate to C:\Program Files\PerkinElmer\ServiceIR\Raman\Videology and double-click on Autorun.exe. The Welcome to Videology USB2.0 Camera Installation Wizard page is displayed.
  • Page 67 Installing Your RamanMicro 200 . 67 38. Click Next. The Please Plug in Your Camera Now page is displayed. 39. Click Next. The Your Camera is Successfully Installed page is displayed. 40. Click Finish. The Videology USB2.0 Camera Software Setup page is displayed.
  • Page 68 68 . RamanMicro 200 Series Getting Started Guide 41. Click Install Viewer. The Welcome to the InstallShield Wizard for Videology USB Viewer is displayed. 42. Click Next. The Choose Destination Location page is displayed. 43. If required, click Change and navigate to your chosen destination folder and then click Next.
  • Page 69 Installing Your RamanMicro 200 . 69 44. Click Install. The Setup Status page is displayed. When the installation is complete, the InstallShield Wizard Complete page is displayed.
  • Page 70 70 . RamanMicro 200 Series Getting Started Guide 45. Click Finish. 46. Restart your PC. This completes the installation of the Spectrum software. If appropriate, you should now install the triggered fiber optic probe drivers, as described in Raman Triggered Fiber Optic Probe leaflet (L1321887) and then connect the fiber optic cables from the triggered fiber optic probe to the RamanMicro 200, as described for a Attaching and Removing the Fiber Optic Probe...

  • Page 71: Alignment Of The Microscope

    Installing Your RamanMicro 200 . 71 Alignment of the Microscope Do not disconnect the microscope from the spectrometer. Only trained PerkinElmer Service Engineers should attach and remove the fiber optic cables that connect the two modules. WARNING The RamanMicro 200 system consists of four primary components: The spectrometer module (containing the laser and the detector);...

  • Page 72: Aligning The Raman Module

    Level (ii) and level (iii) may be carried out without removing any instrument paneling. Level (i) requires removal of instrument paneling and must only be carried out by an appropriately trained PerkinElmer Service Engineer, and in a Class 3B environment. Aligning the Video Camera The crosshairs displayed on the Alignment Camera window in the Spectrum software represent the position of the laser.
  • Page 73 Installing Your RamanMicro 200 . 73 10. Loosen the 4 screws on the top plate of the Raman module until the camera is just free to move (Figure 24). Figure 24 Top view of the microscope showing the screws used to adjust the position of the camera module 11.
  • Page 74 NOTE: Movement of the laser spot in the xy plane may be caused if the surface of the sample is not flat or not mounted correctly. Ensure the sample is flat. PerkinElmer recommends the use of a flat silicon surface.

  • Page 75: Moving The Instrument

    WARNING Please contact PerkinElmer for advice about removing the fiber optic cables that connect the spectrometer and microscope modules. If you need to ship your instrument you must use the original supplied packing materials to prevent damage to the system.
  • Page 76 76 . RamanMicro 200 Series Getting Started Guide...

  • Page 77: Using The Ramanmicro 200

    Using the RamanMicro 200...

  • Page 78: Spectrometer Front Panel (Console)

    78 . RamanMicro 200 Series Getting Started Guide Spectrometer Front Panel (Console) The front panel of the spectrometer module contains 3 LED indicators and 1 key switch (Figure 26). Figure 26 Spectrometer module front panel Description Power If the Power LED is illuminated, this indicates that the instrument is receiving power.

  • Page 79: Fiber Optic Probe

    DO NOT ATTEMPT TO USE THE PROBE BODY IN SUCH AN ENVIRONMENT WITHOUT THE USE OF AN IMMERSION SLEEVE (L1320003). If required, contact PerkinElmer for further details. Attaching and Removing the Fiber Optic Probe The standard fiber optic probe is constructed such that a single armored cable bifurcates into an excitation cable and a collection cable.
  • Page 80 80 . RamanMicro 200 Series Getting Started Guide 5. Connect the excitation cable of the FOP to the coupler marked EXC. Make sure that the polarizing ridge on the FOP cable is aligned with the slot in the coupler casing before carefully pushing the cable into the coupler (Figure 28). Coupler Polarizing ridge aligned with slot...

  • Page 81: Acquiring Spectra With The Fiber Optic Probe

    Using the RamanMicro 200 . 81 Acquiring Spectra with the Fiber Optic Probe The Raman Fiber Optic Probe (L1320002) has a tight focus spot that is approximately 7.5 mm away from the lens. The focused spot enables you to pinpoint the measurement area on the sample.
  • Page 82 However, immersion in solvents will cause serious damage. Ensure an immersion sleeve is in place before immersing your FOP; see Figure 29. Please contact PerkinElmer if you WARNING have any questions in relation to sampling using the FOP.

  • Page 83: General Sops

    Using the RamanMicro 200 . 83 General SOPs The RamanMicro 200 combined with the Spectrum software is a very flexible instrument. This chapter demonstrates how to perform a number of tasks. The procedures provided may not be the most straightforward or simple for the task you wish to perform, so treat these Standard Operating Procedures (SOPs) as a starting point and with time you may wish to develop your own SOPs which better suit your specific mode of use.

  • Page 84: Sop-2: Turning The Instrument Off

    PSU on again. 7. Start the Spectrum software. On the Windows start menu select Programs/PerkinElmer Applications/Spectrum. When you start the software the instrument will prepare for use by turning on the laser and cooling the detector to its operating temperature (usually −50 °C).
  • Page 85 Using the RamanMicro 200 . 85 Position of fixing screw Figure 31 Adjusting the height of the motorized stage 5. Manually move the stage down so that you can replace the objective nosepiece. 6. Replace the objective nosepiece, and then select the longest objective. 7.

  • Page 86: Sop-4: Positioning The Sample On The Microscope

    86 . RamanMicro 200 Series Getting Started Guide SOP-4: Positioning the Sample on the Microscope 1. Lower the microscope sample stage. 2. Place the sample on a microscope slide and place it on the stage. Place your sample here Figure 32 Positioning the sample under the objective lens 3.

  • Page 87: Sop-5: Sample Positioning Using A Motorized Sample Stage

    Using the RamanMicro 200 . 87 14. Select the Dark Field (DF) switch position on the Olympus illuminator module. You are now ready to acquire data. For more information see SOP-6 and SOP-7. Remember, there is no “best” objective. High-magnification objective lenses give the most efficient Raman collection from thin samples, like films or wafers, but low magnification objectives are better for macro samples such as tablets, bulk plastics and tissue samples.

  • Page 88: Sop-6: Setting-Up And Saving Experimental Parameters

    88 . RamanMicro 200 Series Getting Started Guide 5. Use the joystick to move the stage, or click Stage Control and then use the appropriate fields in the Sample Position section to move the stage. The joystick operates in the following manner: •...
  • Page 89 Using the RamanMicro 200 . 89 3. On the Experiment page, select the Set-up tab and then set the Scan time and Number of scans. Typical values for Scan time and Number of scans are 5 seconds and 5 scans, respectively.
  • Page 90 90 . RamanMicro 200 Series Getting Started Guide Table 3 Recommended experimental setup Parameter Recommended Set Up Scan time 5 seconds (sample dependant) (Experiment page/Set-up tab) Number of scans 5 (sample dependant) (Experiment page/Set-up tab) −1 Spectral Information Range 140–3200 cm (Experiment page/Advanced tab) −1 Spectral Information Data Interval...

  • Page 91: Sop-7: Defining Scan Parameters And Starting Data Collection

    Using the RamanMicro 200 . 91 SOP-7: Defining Scan Parameters and Starting Data Collection NOTE: If you are using a Triggered Fiber Optic Probe, refer to the Raman Triggered Fiber Optic Probe leaflet (L1321887) supplied with the probe for configuration and usage details.

  • Page 92: Fiber Optic Probe Sops

    92 . RamanMicro 200 Series Getting Started Guide Fiber Optic Probe SOPs NOTE: The SOPs included on the following pages are only appropriate for a standard fiber optic probe. For details of how to acquire data using a triggered fiber optic probe, see Raman Triggered Fiber Optic Probe leaflet (L1321887).
  • Page 93 Using the RamanMicro 200 . 93 4. Set the Preview Scan time to 1 second, and then press Enter. This will automatically acquire another background. You can enter a Preview Scan time in the range 0.1–10 seconds. The sample may now be positioned and aligned correctly for optimized data collection. The spectral data can be optimized by adjusting the distance between the fiber optic probe and the sample.

  • Page 94: Calibration And Performance Verification Sops

    94 . RamanMicro 200 Series Getting Started Guide Calibration and Performance Verification SOPs Several different automated calibration tests are included in the software, these are: • Wavelength (x-axis) calibration uses the position of precisely known lines in a neon emission spectrum to calibrate the detector. •...
  • Page 95 Then repeat the wavelength calibration verification to ensure that instrument has been brought back within specification. If the instrument fails the wavelength calibration verification again, please contact PerkinElmer.

  • Page 96: Sop-11: Wavelength Calibration Verification Of The Ramanmicro 200

    96 . RamanMicro 200 Series Getting Started Guide SOP-11: Wavelength Calibration Verification of the RamanMicro 200 NOTE: Wavelength Calibration Verification can be carried out using any Beam path. In this example, the Microscope 50x Beam path is used. 1. Ensure the instrument is switched on by following SOP-1. 2.

  • Page 97: Sop-12: Wavelength Calibration Of The Ramanmicro 200

    Using the RamanMicro 200 . 97 12. Select Wavelength Calibration Verification, and then click OK. The wavelength calibration verification routine begins. This will take a few minutes. After completing the wavelength calibration verification routine, an audit log is generated. 13. Remove the polystyrene sample and store safely. The instrument is now ready for use.

  • Page 98: Sop-13: Laser Frequency Calibration Of The Ramanmicro 200

    After completing the wavelength calibration routine, a report is generated on-screen which describes the result of the wavelength calibration. The instrument is now wavelength calibrated. NOTE: If the calibration fails, please contact your PerkinElmer Service Engineer. SOP-13: Laser Frequency Calibration of the RamanMicro 200 Laser frequency calibration should only be performed if the instrument has failed a wavelength calibration verification.

  • Page 99: Sop-14: Intensity Calibration Of The Ramanmicro 200

    The instrument in now laser frequency calibrated. NOTE: If the calibration fails again, please contact your PerkinElmer Service Engineer. SOP-14: Intensity Calibration of the RamanMicro 200 The intensity calibration must be performed using the National Institute of Standards and Technology (NIST) Standard Reference Material.

  • Page 100: Summary

    100 . RamanMicro 200 Series Getting Started Guide 11. Repeat steps 2–10 for each objective lens. 12. Remove the intensity correction standard and store safely. Any data acquired will now be intensity corrected. Remember that if you have a fiber optic probe, you must intensity calibrate the fiber optic probe as well as the microscope objective lenses.

  • Page 101: Additional Parts And Upgrades

    Using the RamanMicro 200 . 101 Additional Parts and Upgrades Microscope Objective Lenses Part Description Number L1323336 100x objective lens L1323347 Long working distance objective lenses upgrade kit Contains 5x, 20x, 50x and 100x long working distance objective lenses Sampling Accessories Part Description Number...

  • Page 102: Safety And Calibration

    Raman probe adjustable spacer kit – for use with L1320030 and L1320031 L1320070 Short immersion sleeve for TFOP – for use with L1320030 and L1320031 L1320071 Ultra-long working distance lens adaptor for FOP For information about customized working distance or cable length fiber optic probes, contact your PerkinElmer Sales Representative.

  • Page 103: Maintenance And Troubleshooting

    Maintenance and Troubleshooting...

  • Page 104: Maintenance

    Avoid spilling liquid into the instrument or accessories. Clean all external spills immediately. If anything that is spilled enters the main body of the spectrometer, make the instrument inoperative and then contact a PerkinElmer Service Engineer. Do not directly clean electrical contacts.

  • Page 105: Cleaning The Air Filters

    Maintenance and Troubleshooting . 105 Cleaning the Air Filters The air filters are located inside the top panel of the spectrometer module. You should clean the filters regularly. To clean the filters: 1. Switch off the instrument power supply unit. 2.

  • Page 106: Servicing The Spectrometer

    Maintenance and Service of the Fiber Optic Probe The FOP is designed to be maintenance free and should not be opened or adjusted by anyone other than a PerkinElmer Service Engineer. Contact PerkinElmer if you have any questions regarding maintenance or servicing of the FOP.

  • Page 107: Replacing The Stage Controller Fuse

    Maintenance and Troubleshooting . 107 Replacing the Stage Controller Fuse NOTE: This section is only applicable if you have a motorized stage. Electrical hazard To prevent potential injury to yourself and damage to the instrument, switch OFF all components in the system and disconnect them from the line power supply before you alter, or make any new, electrical WARNING connections.

  • Page 108: Troubleshooting

    108 . RamanMicro 200 Series Getting Started Guide Troubleshooting This section is a reference guide if you are having any problems with your instrument. Troubleshooting outlines what to do if your instrument or software malfunctions or crashes. Hardware Problem: During the installation procedure the USB devices are not recognized.
  • Page 109 Maintenance and Troubleshooting . 109 Solution When the Welcome to the Found New Hardware Wizard is displayed: 1. Select No, not this time and then click Next. The Found New Hardware Wizard page is displayed. NOTE: The Found New Hardware Wizard page may inform you that it is helping you install software for USB Device.
  • Page 110 110 . RamanMicro 200 Series Getting Started Guide 3. Select Include this location in the search, click Browse, and then navigate to C:\Program Files\PerkinElmer\ServiceIR\Raman. These options may be displayed automatically as you work through the wizard. 4. Click Next. The Completing the Found New Hardware Wizard is displayed.

  • Page 111: Software

    Maintenance and Troubleshooting . 111 Solution 3 If solution 2 is not successful, go to the Spectral Preview tab in the Scan and Instrument Setup dialog, for all motor directions/positions in turn type 1, then press enter, once all positions have been set to 1 and all accessory movements are complete, disconnect the accessory from its electrical connector in the sample compartment (there is no need to physically remove the accessory).
  • Page 112 Add or Remove Programs. Then remove the Philips ToUcom Pro Camera, the Raman Instrument and then Spectrum Software (in that order). You should also delete files C:\pel_apps\, C:\pel_data, C:\Documents and Settings\All Users\Application Data\PerkinElmer\SecuritySystem, C:\Windows\pel_apps.ini and C:\Windows\pe_sopb.ini using Windows Explorer. To install the new version of Spectrum software follow the instructions in Installing Spectrum Software on page 54.

  • Page 113: Decontamination

    (8:30 a.m. – 7 p.m. EST ) (+1) 203-925-4602 (outside the USA) Customer Care Canada: 800-561-4646 Customer Care EU: 0800 40 858 (Brussels) 0800 90 66 42 (Monza) If you are located outside of these regions, please call your local PerkinElmer sales office for more information.

  • Page 114: Weee Instructions For Perkinelmer Products

    Customer Care department in your region. http://las.perkinelmer.com/OneSource/Environmental-directives.htm Products from other manufacturers may also form a part of your PerkinElmer system. These other manufacturers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive. Please contact these manufacturers directly before discarding any of their products.

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