Shodex KW400-4F Series Operation Manual

(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex™ product. Shodex PROTEIN KW400-4F series is a silica-based gel
filtration chromatography column. The column series is suitable for the size exclusion separations of proteins.
The columns with different exclusion limits are available. Please select columns with target molecular weight
ranges (see below) that meet the molecular weight range of your samples.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6989201 KW402.5-4F
F6989202 KW403-4F
F6989203 KW404-4F
F6989204 KW405-4F
F6700132 KW400G-4A
Base Material : Spherical porous particles of silica modified with hydrophilic group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
Operation Manual

Shodex KW400-4F series
Column Size (mm)
Particle
Size (μm)
I.D. Length
4.6 300 3
4.6 300 3
4.6 300 5
4.6 300 5
4.6 10 5
SHOWA DENKO K.K. (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 35,000 5,000 - 70,000
≥ 35,000 10,000 - 500,000
≥ 25,000 30,000 - (4,000,000)
≥ 25,000 200,000 - (20,000,000)
(Guard Column)
*1
Reference value only / Measured with protein
ver.211111E
Exclusion Limit
*1
150,000
600,000
*2 (4,000,000)
*2
(20,000,000)
- -
*2
Estimated value
*1
*2
*2
1