Shodex KW400-4F Series Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex KW400-4F series is silica-based gel filtration
chromatography columns. The column series is suitable for size exclusion separations of proteins. The columns
with different exclusion limits are available. Please select columns with target molecular weight ranges (see
below) that meet the molecular weight range of your samples.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
KW402.5-4F
F6989201
F6989202 KW403-4F
F6989203 KW404-4F
F6989204 KW405-4F
F6700132 KW400G-4A
Base Material : Spherical porous particles of silica modified with hydrophilic group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
Operation Manual
Shodex KW400-4F series
Column Size
Particle
(mm)
Size
(μm)
I.D. Length
4.6 300 3
4.6 300 3
4.6 300 5
4.6 300 5
4.6 10 5
Resonac Corporation (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 35,000 5,000 - 70,000
≥ 35,000 10,000 - 500,000
≥ 25,000 30,000 - (4,000,000)
≥ 25,000 200,000 - (20,000,000)
(Guard Column)
*1 Reference value only / Measured with proteins
ver.20221202E
Exclusion
*1 Limit *1
150,000
600,000
(4,000,000)
*2 *2
*2 (20,000,000) *2
- -
*2 Estimated value
1