Related Manuals for Endress+Hauser Analytik Jena Biometra TAdvanced 96
Summary of Contents for Endress+Hauser Analytik Jena Biometra TAdvanced 96
- Page 1 Operating Manual Biometra TAdvanced High-performance thermal cycler for DNA amplification by PCR...
- Page 2 Biometra GmbH (Analytik Jena AG) Manufacturer Rudolf-Wissell-Str. 30 37079 Göttingen Germany Phone + 49 551 50 68 6-0 + 49 551 50 68 6-66 Email Info@analytik-jena.com Biometra GmbH Service Rudolf-Wissell-Str. 30 37079 Göttingen Germany Phone + 49 551 50 68 6 -10, -14 or -16 Email Service@analytik-jena.com http://www.analytik-jena.com...
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Page 3: Table Of Contents
Biometra TAdvanced Manual Contents Contents Basic information ....................... 9 User manual notes ......................9 Intended use ........................10 Warranty and liability ..................... 10 Safety instructions ....................11 General notes ........................11 Safety markings on the Biometra TAdvanced .............. 11 Technical condition ......................11 Requirements for the operating personnel .............. - Page 4 Contents Biometra TAdvanced Manual Creating, editing and saving programs ..............44 Programming modes ...................... 44 Creating a new program/using a program template ............ 45 Assigning program names....................47 Setting the heated lid temperature ................47 Preheating the heated lid ....................48 Editing steps ........................
- Page 5 Biometra TAdvanced Manual Contents Fault removal ......................116 14.1 Administrator password forgotten ................116 14.2 Slow heating and cooling ..................... 116 14.3 Auto restart ........................116 14.4 Auto restart without an apparent cause ..............116 14.5 Adaptation of programs from other thermal cyclers ..........117 14.6 Loosening the lid wheel in case of blockage ...............
- Page 6 Contents Biometra TAdvanced Manual Fig. 28 Program step editing screen ................. 50 Fig. 29 PCR program with a loop ................51 Fig. 30 Temperature gradient on a 96 well sample block ........52 Fig. 31 Linear Gradient Tool screen (Biometra TAdvanced Mono)......53 Fig.
- Page 7 Biometra TAdvanced Manual Contents Fig. 82 User management screen ................94 Fig. 83 Configure user management screen ............. 94 Fig. 84 Create new user screen .................. 96 Fig. 85 User data screen ..................... 97 Fig. 86 User properties screen ................... 98 Fig.
- Page 8 Contents Biometra TAdvanced Manual Version 02_20...
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Page 9: Basic Information
Biometra TAdvanced Basic information Basic information User manual notes The Biometra TAdvanced is intended for operation by qualified specialist personnel observing this operating manual. For the Biometra TAdvanced, there are 12 different models available. In the text below these devices are collectively called Biometra TAdvanced. Differences are explained in the corresponding sections. -
Page 10: Intended Use
Basic information Biometra TAdvanced Symbol for waste disposal in accordance with WEEE directive: Do not dispose of in household waste. CE marking China RoHS marking Intended use The Biometra TAdvanced is an end-point thermal cycler. It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reaction. -
Page 11: Safety Instructions
Biometra TAdvanced Safety instructions Safety instructions General notes For your own safety and to ensure error-free and safe operation of the Biometra TAdvanced, please read this section carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the operation of the device, the general applicable regulations regarding accident prevention, occupational health and safety and environmental protection have to be observed and complied with. -
Page 12: Requirements For The Operating Personnel
Safety instructions Biometra TAdvanced The following has to be observed: The operator must only operate the device in a sound and operationally safe condition. The technical condition must always comply with the legal requirements and regulations. Prior to every use the device must be checked for damage and sound condition. ... -
Page 13: Safety Instructions: Protection Against Explosion And Fire
Biometra TAdvanced Safety instructions The ventilation fittings at the rear of the device must be unobstructed and operational. Covered ventilation grilles or slits etc. may cause the device to break down or may cause damage to it. The use of oil between the samples and the sample block is not necessary to achieve an improved heat exchange. -
Page 14: Handling Of Samples, Auxiliary And Operating Materials
Safety instructions Biometra TAdvanced Do not operate the device near sources of strong electromagnetic radiation (for example, an unshielded, deliberately operated high frequency source), as these may affect proper operation of the device. 2.6.4 Handling of samples, auxiliary and operating materials The operator is responsible for the selection of substances used in the process as well as for their safe handling. -
Page 15: Function And Setup
Biometra TAdvanced Function and setup Function and setup The Biometra TAdvanced thermal cycler is available in the following models: Model Biometra TAdvanced 96 with aluminum block Biometra TAdvanced 96 G with aluminum block with gradient function Biometra TAdvanced 96 S with silver block Biometra TAdvanced 96 SG with silver block... -
Page 16: High Speed Thermoblock
Function and setup Biometra TAdvanced The Biometra TAdvanced thermal cycler features an automatic restart. If a power failure occurs during the run, the device will continue the run as soon as power is restored. In case of long-term power failure (longer than 30 minutes), the device will keep the sample block at 4 °C (freeze step) and the user can decide to repeat the run with the same samples or to discard them. -
Page 17: Display
Biometra TAdvanced Function and setup Display The Biometra TAdvanced thermal cycler features a 7" state-of-the-art color touchscreen user interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing and ergonomic programming. For programming PCR protocols the software incorporates Biometra's proven spreadsheet philosophy and alternatively offers graphical programming. -
Page 18: Linear Gradient Tool
Function and setup Biometra TAdvanced Linear Gradient Tool For optimizing new primer pairs in a single run the Biometra TAdvanced can optionally be equipped with a gradient function. Finding the best primer annealing temperature is crucial for the specificity and efficiency of PCR reactions. Often the optimization of experiments is conducted to a limited extent only leading to unspecific byproducts or reduced PCR sensitivity. -
Page 19: Order Numbers
Biometra TAdvanced Function and setup Order numbers Instruments incl. block and base unit Model Block type Gradient range Block exchange Order numbers Biometra TAdvanced 96 Aluminum – 846-x-070-211 Biometra TAdvanced 96 G Aluminum 30 °C 846-x-070-201 Biometra TAdvanced 96 S Silver –... -
Page 20: Installation And Commissioning
Installation and commissioning Biometra TAdvanced Installation and commissioning Scope of delivery The scope of delivery includes: Thermal cycler Mains cable Operating instructions Quick reference guide Please keep the original packaging in case the device needs to be returned. The shipping box for the Biometra TAdvanced thermal cycler contains a specially developed foam system for the transport of high quality electronic devices. -
Page 21: Commissioning
Biometra TAdvanced Installation and commissioning Commissioning WARNING Danger of electric shock! Prior to commissioning, please make sure that the operating voltage set on the device is the same as the mains voltage (see section "Setting the operating voltage" p. 20). NOTICE Do not cover the ventilation slits! Ensure that the ventilation slits are clear. -
Page 22: Control Elements
Control elements Biometra TAdvanced Control elements Biometra TAdvanced front view Fig. 3 Front view of the Biometra TAdvanced Dial for adjusting the contact pressure Display Lid closing button USB port Version 02_20... -
Page 23: Biometra Tadvanced Rear View
Biometra TAdvanced Control elements Biometra TAdvanced rear view The Ethernet port, mains plug and mains switch are located at the rear of the Biometra TAdvanced. Fig. 4 Rear view of the Biometra TAdvanced Ethernet port Fuse holder Mains plug Mains switch Quick block exchange The option for quick block exchange is available for all model types. -
Page 24: High Performance Smart Lid (Hpsl)
Control elements Biometra TAdvanced Fig. 5 Quick block exchange High Performance Smart Lid (HPSL) The Biometra TAdvanced has a height-adjustable lid for optimal contact pressure on the samples. 5.4.1 Closing the lid 1. Place the samples in the block, then close the lid until you feel the button in the front click into position. -
Page 25: Opening The Lid
Biometra TAdvanced Control elements Fig. 6 Placement of sample tubes in different block systems 5.4.2 Opening the lid NOTICE Never open the lid under pressure! Always follow the sequence below when opening the lid. If the lid is opened under pressure, the locking mechanism may be damaged. -
Page 26: The Biometra Tadvanced Software
Control elements Biometra TAdvanced The Biometra TAdvanced software The Biometra TAdvanced thermal cycler features a 7" color touchscreen and a completely new software for operation. 5.5.1 Keyboard For some applications, names, addresses or passwords have to be defined. For this purpose, the Biometra TAdvanced software offers a keyboard to enter characters, digits and special characters. -
Page 27: Keypad
Biometra TAdvanced Control elements 5.5.2 Keypad In some screens of the Biometra TAdvanced software digits have to be entered. For this purpose, the software features a keypad integrated in some screens: Fig. 8 Keypad The keypad can be used to enter digits, dots, minus signs and infinite. In addition the following special keys are available: ELETE SINGLE CHARACTER ELETE ALL CHARACTERS... -
Page 28: List Of Commands And Icons Used
Control elements Biometra TAdvanced 5.5.5 List of commands and icons used The Biometra TAdvanced software uses the following icons: Command Icon IGHT ONFIRM ANCEL OGIN SER MANAGEMENT SER MANAGEMENT ON/OFF EW USER ELETE USER DIT USER ROTOCOL IZARD OLYMERASE OLYMERASE ELETE OLYMERASE Version 02_20... - Page 29 Biometra TAdvanced Control elements Command Icon NNEALING TEMPERATURE CALCULATOR DIT PRIMER CONCENTRATION DIT PRIMER ALCULATE PRIMER AVE ANNEALING TEMPERATURE ROTOCOL IZARD NCUBATION TART INCUBATION ROGRAMS EW PROGRAM FROM TEMPLATE PEN TEMPLATE OPY PROGRAM COPY ALL PROGRAMS ELETE PROGRAM DELETE ALL PROGRAMS DIT PROGRAM TART PROGRAM UICK SELECTION...
- Page 30 Control elements Biometra TAdvanced Command Icon AVE PROGRAM AVE AS AUSE PROGRAM TART PROGRAM CONTINUE PROGRAM KIP STEP HOOSE BLOCK START PROGRAM LOCK STOP RAPHICAL VIEW PREADSHEET VIEW RADIENT DISPLAY DIT STEP NSERT STEP ELETE STEP EMPERATURE INCREMENT IME INCREMENT EATING RATE RADIENT INEAR...
- Page 31 Biometra TAdvanced Control elements Command Icon REHEATING REHEATING OOLS ETTINGS ATE AND TIME UTOMATIC USER LOGOUT EEPER ETWORK ISPLAY BRIGHTNESS ISPLAY DARKER ISPLAY BRIGHTER OUCHSCREEN CALIBRATION ACTORY SETTINGS OCUMENTATION UN LOG FILE UN LOG FILE OVERVIEW AVE RUN LOG FILE TO AVE ALL RUN LOG FILES IEW RUN LOG FILE Version 02_20...
- Page 32 Control elements Biometra TAdvanced Command Icon IEW PROGRAM OWER ON LOG FILE TEST LOG FILE RROR LOG FILE XTENDED SELF TEST TART EXTENDED SELF TEST TOP EXTENDED SELF TEST ERVICE INFO FILE ACKUP AVE BACKUP FILE OAD BACKUP FILE ELETE BACKUP FILE ELETE ALL BACKUP FILES YCLER INFORMATION...
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Page 33: Operation
Biometra TAdvanced Operation Operation Power on self-test After the Biometra TAdvanced is switched on, the device performs an automatic self- test (power on self-test). During the test the device shows the following screen: Fig. 9 Start screen For the power on self-test a log file is created and stored in the thermal cycler memory (see section "Power On Logfile"... -
Page 34: Login
Operation Biometra TAdvanced Biometra TAdvanced Mono Biometra TAdvanced Twin Fig. 10 Login screen Login After starting the Biometra TAdvanced, the login screen is displayed. To log in as an existing user, press OGIN In the next screen select a user by pressing the corresponding button. Fig. -
Page 35: Home Screen
Biometra TAdvanced Operation Fig. 12 Enter password screen Home screen After user login the Biometra TAdvanced software shows the home screen. The home screen offers the following functions: Biometra TAdvanced Mono Biometra TAdvanced Twin Fig. 13 Home screen Version 02_20... -
Page 36: Block Status
Operation Biometra TAdvanced Command Function Section Viewing, running and editing programs See section "Creating, editing ROGRAMS and saving programs" p. 44 Shows the current block status. If a block is See section "Block status" LOCK free, a touch on the button leads to a list p. -
Page 37: Quick Start Function
Biometra TAdvanced Operation Button Block status Color Function Free Green Quick start function Running Yellow Program view Pause Blue Program view Quick start function Note: Programs can only be started by users with the right S Biometra TAdvanced TART STOP PROGRAMS activated (see section "Editing user settings"... -
Page 38: Fig. 15 Program Quick Start Screen (Biometra Tadvanced Mono)
Operation Biometra TAdvanced Fig. 15 Program quick start screen (Biometra TAdvanced Mono) Select the program for quick start in the table and press the [S ] button to start the TART selected program. If necessary, the program can be edited before the start or a new program can be created based on a template (see section "Programming modes"... -
Page 39: Fig. 16 Program Quick Start Screen (Biometra Tadvanced Twin)
Biometra TAdvanced Operation login screen (see section "Login screen" p. 33) or in the home screen (see section "Home screen" p. 35). Note: The quick start function can only be activated if the current block status is F (see section "Quick start function" p. 37). If the status is R or P , a touch of UNNING... -
Page 40: Incubation Mode
Operation Biometra TAdvanced HOOSE BLOCK START PROGRAM By activating the checkboxes select the sample blocks for program start. Fig. 17 Multiblock selection query Note: Programs offered for quick start can only be edited or deleted by users with the right W activated (see section "Editing user settings"... -
Page 41: Fig. 18 Incubation Mode Screen (Biometra Tadvanced Mono)
Biometra TAdvanced Operation Note: To set the time to endless, use the button: NDLESS TIME To start incubation, press TART Fig. 18 Incubation mode screen (Biometra TAdvanced Mono) During incubation the following commands can be used: AUSE ONTINUE Note: To stop the incubation, use the corresponding command. Do not switch off the device while the incubation is running. -
Page 42: Fig. 19 Incubation Mode Screen (Biometra Tadvanced Twin)
Operation Biometra TAdvanced NABLE A USER TO START OR STOP PROGRAMS The incubation mode allows samples to be incubated at a constant temperature for an indefinite period of time. Press the [I ] button and in the next screen set the NCUBATE values for the block temperature and hold time using the keypad. -
Page 43: Fig. 20 Multiblock Selection Query (1)
Biometra TAdvanced Operation To start incubation on both sample blocks press the following button: HOOSE BLOCK START PROGRAM By activating the checkboxes select the sample blocks for program start. Fig. 20 Multiblock selection query (1) During incubation the following commands can be used: LOCK AUSE ONTINUE... -
Page 44: Creating, Editing And Saving Programs
Creating, editing and saving programs Biometra TAdvanced Creating, editing and saving programs Programming modes The Biometra TAdvanced software offers spreadsheet or graphical programming. Use the buttons to toggle between the two modes. The following screenshots show the spreadsheet and graphical programming mode. RAPHICAL VIEW PREADSHEET VIEW Fig. -
Page 45: Creating A New Program/Using A Program Template
Biometra TAdvanced Creating, editing and saving programs Both programming modes show the same information but in different ways. In the spreadsheet mode all parameters are summarized in a table. In the graphical mode symbols are displayed for some parameters (see the table below). Toggle to the spreadsheet mode to view the settings for these parameters. -
Page 46: Fig. 24 Program Overview Screen
Creating, editing and saving programs Biometra TAdvanced 2. After login press the [P ] button in the home screen. ROGRAM ROGRAM The program overview screen opens: Fig. 24 Program overview screen 3. In the program overview screen, press the [N ] button: EW FROM TEMPLATE EW FROM TEMPLATE... -
Page 47: Assigning Program Names
Biometra TAdvanced Creating, editing and saving programs Select B whenever a new program should be created and select another template LANK from the list whenever an existing program should be edited. The program structure can be controlled in the program preview (see Fig. 26). If a template is selected, the program steps and some additional information are displayed in spreadsheet form. -
Page 48: Preheating The Heated Lid
Creating, editing and saving programs Biometra TAdvanced Fig. 27 Heated lid temperature setting screen NOTICE The heated lid automatically switches off at a temperature difference of >75 °C between the block and the heated lid. At these low block temperatures, sample condensation on the tube lid is no longer to be expected. -
Page 49: Editing Steps
Biometra TAdvanced Creating, editing and saving programs Editing steps When editing program steps, the following parameters can be entered in the Biometra TAdvanced software: Parameter Description and limits Temperature for a step in °C. The target temperature can be set between 3.0°C and 99.0°C in EMPERATURE tenths of a degree. - Page 50 Creating, editing and saving programs Biometra TAdvanced Fig. 28 Program step editing screen Note: To program a step at least the temperature and the hold time must be defined. 3. To set the temperature, touch the corresponding input field and enter a value between 3.0°C and 99.0°C.
- Page 51 Biometra TAdvanced Creating, editing and saving programs Fig. 29 PCR program with a loop In this example, the device first executes steps 1 to 4, then repeats step 2 to step 4 for 34 times, performs the final extension at 72°C and holds the temperature indefinitely at 16°C in the last step.
- Page 52 Creating, editing and saving programs Biometra TAdvanced The average ramp rate can be adjusted for each step. This adjustment can be useful if Adjusting the ramp rate PCR programs are transferred from slower devices to the Biometra TAdvanced or for special PCR applications, for example, telomerase PCR.
- Page 53 Biometra TAdvanced Creating, editing and saving programs RADIENT There are two options to program a gradient. To toggle between the two options, press the corresponding button on the Biometra TAdvanced gradient programming screen (see Fig. 31): INEAR RADIENT TANDARD RADIENT ROGRAMMING To program a gradient using the Linear Gradient Tool, enter the desired annealing temperature (A...
- Page 54 Creating, editing and saving programs Biometra TAdvanced Fig. 32 Standard gradient programming screen (Biometra TAdvanced Mono) For Biometra TAdvanced twinblock models Note: To be able to use the gradient function, the sample block needs to be gradient enabled. For Biometra TAdvanced Twin 48 G this is the left block. The gradient function is most often used to optimize new primer pairs.
- Page 55 Biometra TAdvanced Creating, editing and saving programs There are two options to program a gradient. To toggle between the two options, press the corresponding button on the Biometra TAdvanced gradient programming screen (see Fig. 31): INEAR RADIENT TANDARD RADIENT ROGRAMMING To program a gradient using the Linear Gradient Tool, enter the desired annealing temperature (A .) and define a temperature increment (G...
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Page 56: Direct Programming Of Program Parameters For A Step
Creating, editing and saving programs Biometra TAdvanced Fig. 35 Standard gradient programming screen (Biometra TAdvanced Twin) Note: Programs with gradient steps can only be started on blocks with gradient function. Please note that for twinblock models only the left block (block 1) features the gradient function. - Page 57 Biometra TAdvanced Creating, editing and saving programs Fig. 37 Spreadsheet program view Parameter Spreadsheet view Temperature (gradient) [°C] ① Hold time [h:mm:ss] ② Loops ③ Number of cycles ④ Temperature increment [∆T (°C)] ⑤ Time increment [∆t (s)] ⑥ Ramp rate [∆R (°C/s)] ⑦...
- Page 58 Creating, editing and saving programs Biometra TAdvanced Fig. 38 Graphical program view Parameter Spreadsheet view Temperature (gradient) [°C] input field ① Hold time [h:mm:ss] input field ② Loop counter option button ③ Temperature increment option button ④ Time increment option button ⑤...
- Page 59 Biometra TAdvanced Creating, editing and saving programs Fig. 39 Individual program parameter editing If the sample block is gradient-enabled, you also have the option of programming a gradient step. To do this, when editing the temperature, press the G button. RADIENT A gradient step can be programmed here (see Fig.
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Page 60: Inserting A Step
Creating, editing and saving programs Biometra TAdvanced Inserting a step To insert a step, select a program step on the Biometra TAdvanced spreadsheet or graphical programming screen and press the [I ] button. NSERT NSERT When you press the [I ] button, the screen for editing program steps opens (see NSERT section "Editing steps"... - Page 61 Biometra TAdvanced Creating, editing and saving programs Note: If a new program is to be saved, the first free storage location in the directory of the currently logged in user is automatically selected. However, the storage location can be changed using the cursor keys above and below the lists. If the selected storage location is already in use, the stored program will not be overwritten;...
- Page 62 Creating, editing and saving programs Biometra TAdvanced Fig. 42 Quick selection screen Before saving the program there is still the possibility of changing the program name. To do this, press the [S ] button and enter the new program name using the AVE AS Biometra TAdvanced keyboard (see section "Assigning program names"...
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Page 63: Starting, Coping And Deleting Programs
Biometra TAdvanced Starting, coping and deleting programs Starting, coping and deleting programs Starting a program Note: Programs can only be started by users with the right S TART STOP PROGRAMS activated (see section "Editing user settings" p. 96). Additionally the right R EAD ALL defines, if a user is allowed to read all programs or if the access is limited to PROGRAMS... - Page 64 Starting, coping and deleting programs Biometra TAdvanced Touch the corresponding buttons to select a user directory and a stored program. If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down.
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Page 65: Copying A Program
Biometra TAdvanced Starting, coping and deleting programs Copying a program Note: Users can only copy and save their own programs when the right W RITE DELETE is activated. Programs of others can be copied and saved with the right OWN PROGRAMS activated (see section "Editing user settings"... -
Page 66: Copying All Programs
Starting, coping and deleting programs Biometra TAdvanced Fig. 46 Program overview screen Copying all programs Note: Users can only copy and save their own programs when the right W RITE DELETE is activated. Programs of others can be copied and saved with the right OWN PROGRAMS activated (see section "Editing user settings"... -
Page 67: Deleting A Program
Biometra TAdvanced Starting, coping and deleting programs Note: The programs will be copied into the selected user directory beginning at the first free storage location. Fig. 47 Program overview screen Deleting a program Note: Users can only delete their own programs when the right W RITE DELETE OWN is activated. -
Page 68: Deleting All Programs
Starting, coping and deleting programs Biometra TAdvanced Fig. 48 Confirmation prompt Note: If a program is deleted, all the following programs will be moved one storage location lower. The storage location number for these programs will decrease by -1. Deleting all programs Note: Users can only delete their own programs when the right W RITE DELETE OWN... -
Page 69: Running, Pausing, Continuing And Stopping Programs
Biometra TAdvanced Running, pausing, continuing and stopping programs Running, pausing, continuing and stopping programs To switch the display of the sample blocks during a run press the button with the Biometra TAdvanced number of the corresponding sample block. Twin AMPLE BLOCK AMPLE BLOCK Display during a run After a program has been started (see section "Starting a program"... - Page 70 Running, pausing, continuing and stopping programs Biometra TAdvanced Fig. 51 Spreadsheet view Fig. 52 Display during the run in a graphical, spreadsheet and gradient view Version 02_20...
- Page 71 Biometra TAdvanced Running, pausing, continuing and stopping programs Biometra TAdvanced Twin Fig. 53 Graphical view Fig. 54 Spreadsheet view Version 02_20...
- Page 72 Running, pausing, continuing and stopping programs Biometra TAdvanced Fig. 55 Display during the run in a graphical, spreadsheet and gradient view The following parameters are summarized in the headline in the table: Program name Sample block temperature Estimated end ...
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Page 73: Pausing A Program
Biometra TAdvanced Running, pausing, continuing and stopping programs Pausing a program To pause a program, press the [P ] button on the Biometra TAdvanced run display AUSE screen (see Fig. 52 p. 44). AUSE The word "Pause" is shown and the pause time is counted in the R field. - Page 74 Running, pausing, continuing and stopping programs Biometra TAdvanced Biometra TAdvanced Twin Fig. 58 Spreadsheet view Fig. 59 Display screen during a program pause (graphical view) Note: Pressing the [P ] button requires manual interaction. The Biometra AUSE TAdvanced software also allows pauses to be programmed (see section "Editing steps" p.
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Page 75: Continuing The Program
Biometra TAdvanced Running, pausing, continuing and stopping programs Continuing the program To continue a paused program, press the C button on the Biometra TAdvanced ONTINUE run display screen (see Fig. 57 p. 73). ONTINUE The Biometra TAdvanced will continue the program from exactly the point at which it was paused. - Page 76 Running, pausing, continuing and stopping programs Biometra TAdvanced Fig. 60 Confirmation prompt After confirmation of the confirmation prompt the Biometra TAdvanced stops the current program. If two sample blocks are running the Biometra TAdvanced offers the option to stop Biometra TAdvanced only one block or to stop both blocks simultaneously.
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Page 77: Protocol Wizard
Biometra TAdvanced Protocol Wizard Protocol Wizard The protocol wizard is a helpful tool for automatic creation of PCR programs and primer annealing temperature calculation. To start the protocol wizard press the following button in the Biometra TAdvanced home screen (see section "Home screen" p. -
Page 78: Enter A New Polymerase Or Edit A Polymerase
Protocol Wizard Biometra TAdvanced 10.2 Enter a new polymerase or edit a polymerase To enter program templates for new polymerases or to edit preset program templates for polymerases press the corresponding button: EW POLYMERASE DIT POLYMERASE In the next screen (see Fig. 64) touch the corresponding input boxes to enter a name for the polymerase and set parameters for all requested program steps. - Page 79 Biometra TAdvanced Protocol Wizard Fig. 65 Select polymerase screen Note: If a new or edited polymerase is to be saved, the first free storage place in the polymerase list is pre-selected. However, the storage place can be modified by using the cursor key function above and below the list.
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Page 80: Delete A Polymerase
Protocol Wizard Biometra TAdvanced 10.3 Delete a polymerase To delete a polymerase press the [ ] button. DELETE ELETE OLYMERASE Note: The Template I cannot be deleted. 10.4 Protocol Wizard parameter screen For automatic calculation of PCR programs in the protocol wizard parameter screen (see Fig. - Page 81 Biometra TAdvanced Protocol Wizard 6. To set the parameters touch the corresponding field in the protocol wizard parameter screen and enter the desired values. Fig. 67 Protocol wizard parameter screen 7. After setting all program parameters press the [N ] button. The next screen lists the all steps of the program that will be created by the software: Fig.
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Page 82: Primer Annealing Temperature Calculator
Protocol Wizard Biometra TAdvanced program created by the protocol wizard follow the instructions in section "Saving the program" p. 60. REATE PROGRAM Note: Programs have to be saved before they can be started. If the program has been created but should not be saved confirm the following security query to discard the program: Fig. - Page 83 Biometra TAdvanced Protocol Wizard Fig. 70 Primer annealing temperature calculator screen To adjust the primer or salt concentration press the [E ] button. DIT CONCENTRATION DIT CONCENTRATION In the next screen the primer concentration, salt concentration and magnesium concentration can be adjusted. Touch the corresponding input field and enter values using the keypad.
- Page 84 Protocol Wizard Biometra TAdvanced Press the [E ] button to toggle to the primer annealing temperature DIT PRIMER calculator screen (see Fig. 70) or press the [C ] button to calculate the PRIMER theoretical primer annealing temperature (T RIMER PRIMER The calculated values for the forward primer melting temperature (T ), reverse primer melting temperature (T...
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Page 85: Tools
Biometra TAdvanced Tools Tools The Biometra TAdvanced software provides the following tools: Defines basic device settings See section "Configuration" p. 85 ETTINGS Tool for user management See section "User management" p. 93 ANAGEMENT Access to stored log files See section "Documentation" p. 99 OCUMENTATION Executes the extended self-test See section "Extended self-test"... - Page 86 Tools Biometra TAdvanced The Biometra TAdvanced software offers the following setting options: Sets the date and time See section "Setting the date and time" ATE AND TIME p. 87 Switches automatic user logout on or off and See section "Automatic user logout" UTOMATIC USER LOGOUT time settings p.
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Page 87: Setting The Date And Time
Biometra TAdvanced Tools 11.1.1 Setting the date and time The Biometra TAdvanced software allows the user to set the date and time. The date has the format and the time the format MONTH YEAR HOURS MINUTES SECONDS Fig. 74 Date and time entry screen Activate the corresponding input field and use the keypad on the right side of the date and time entry screen to enter digits. -
Page 88: Automatic User Logout
Tools Biometra TAdvanced 11.1.2 Automatic user logout Note: The automatic user logout can only be configured by users with the right S YSTEM activated (see section "Editing user settings" p. 96). CONFIGURATION NABLE A USER TO CONFIGURE THE SYSTEM CONFIGURATION The Biometra TAdvanced features an automatic user logout function. -
Page 89: Configuring The Beeper
Biometra TAdvanced Tools 11.1.3 Configuring the beeper Note: The beeper can only be configured by users with the right S YSTEM CONFIGURATION activated (see section "Editing user settings" p. 96). NABLE A USER TO CONFIGURE THE SYSTEM CONFIGURATION The Biometra TAdvanced has a beeper which, if switched on, will issue an acoustic signal when a PCR program is finished. - Page 90 Tools Biometra TAdvanced If DHCP (Fig. 78) is activated, the device automatically receives the network configuration settings. If the static checkbox is activated, you can enter the IP address and the subnet mask using the keypad on the right side of the screen. The port number can entered manually in both setting types (static and DHCP).
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Page 91: Display Brightness
Biometra TAdvanced Tools The software stores the users who access the Biometra TAdvanced via the network Network users and lists them in chronological order in a table. The function makes it possible to check if only authorized persons have network access to the device. The app is available for iOS (Apple App Store) and Android (Google Play Store) driven PCR control App systems. -
Page 92: Screen Calibration
Tools Biometra TAdvanced Fig. 79 Display brightness adjustment screen 11.1.6 Screen calibration Note: The touch screen can only be calibrated by users with the right S YSTEM activated (see section "Editing user settings" p. 96). CONFIGURATION NABLE A USER TO CONFIGURE THE SYSTEM CONFIGURATION To calibrate the screen, follow the instructions and touch the screen at the center of the displayed circle. -
Page 93: Factory Settings
Biometra TAdvanced Tools 11.1.7 Factory settings Note: Factory settings can only be reset by users with the right S YSTEM CONFIGURATION activated (see section "Editing user settings" p. 96). NABLE A USER TO CONFIGURE THE SYSTEM CONFIGURATION Press the [F ] button and confirm the following confirmation prompt: ACTORY SETTINGS Fig. - Page 94 Tools Biometra TAdvanced user management. Also with the user management switched off the administrator has to log in. DMIN Fig. 82 User management screen In the next screen activate the corresponding checkbox to configure the Biometra TAdvanced user management: Fig. 83 Configure user management screen If the user management is switched off, all new users have all rights and can access all software functions.
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Page 95: Creating A User
Biometra TAdvanced Tools Administrator User with generic User with limited rights rights RITE DELETE ALL PROGRAMS RITE DELETE OWN PROGRAMS EAD ALL PROGRAMS TART STOP PROGRAMS HOW YOUR OWN PROGRAMS TO OTHER USERS * An administrator can create users of all groups and modify their rights settings. A user with generic rights is only allowed to create users with generic or limited rights but no administrator. -
Page 96: Editing User Settings
Tools Biometra TAdvanced To create a new user, press the [N ] button. EW USER In the next screen enter the using the Biometra TAdvanced keyboard (see section "Keyboard" p. 26) (up to 13 characters or numbers) SERNAME (2 to 3 characters) ... - Page 97 Biometra TAdvanced Tools CCOUNT On the next screen the user data and the language settings can be changed. Fig. 85 User data screen Note: For the administrator A the user name and the initials ADM cannot be DMIN modified. It is recommended to change the password for the administrator A after DMIN the first system start and to create a backup of the system.
- Page 98 Tools Biometra TAdvanced Fig. 86 User properties screen Right Function Provides access to several system settings like date and time, YSTEM CONFIGURATION screen saver, beeper, network settings, display brightness, touch screen calibration and factory settings Allows to modify the user name, user initials, password, DIT USER language setting and user rights Allows to create new user accounts.
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Page 99: Delete User
Biometra TAdvanced Tools 11.2.3 Delete user Note: User accounts can only be deleted by users with the right D activated. ELETE NABLE A USER TO DELETE USERS To delete a user, select the user on the user management screen (see section "User management"... -
Page 100: Run Log File
Tools Biometra TAdvanced Fig. 88 Documentation screen Press the corresponding button to access the desired function. 11.3.1 Run log file The Biometra TAdvanced lists up to 24 run log files for the programs that were last started. Fig. 89 Run log file overview screen The run log files are sorted by date and time. - Page 101 Biometra TAdvanced Tools To view the collected data, select a run log file by activating the corresponding row in the table and press the [V ] button. In the run log file screen the following information is listed: Name of the run log file ...
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Page 102: Power On Logfile
Tools Biometra TAdvanced RAPHICAL VIEW PREADSHEET VIEW Fig. 91 Program view screen Use the buttons to toggle between the overview screen, the run log file screen and the view program screen. VERVIEW ILES ROGRAM To export all the saved run log files on USB, press the [S USB] AVE ALL ILES ON... -
Page 103: Extended Self-Test Log File
Biometra TAdvanced Tools Last power-down by date and time A table with a list of error messages by number, date, time, error code and message Fig. 92 Power on log file screen 11.3.3 Extended self-test log file During the extended self-test the Biometra TAdvanced checks several functions and components like cooler, thermal tracking, heating and cooling rate, refrigeration, gradient, heated lid and regulation (see section "Extended self-test"... -
Page 104: Error Log File
Tools Biometra TAdvanced 11.3.4 Error log file The Biometra TAdvanced records all errors by date and time. The latest error is shown at the first position in the error table. The error code and the message are also listed. Fig. 94 Error log file screen 11.4 Extended self-test NOTICE... - Page 105 Biometra TAdvanced Tools Test Function Gradient Checks if the sample block reaches the set gradient temperatures. Heated lid Tests if the heated lid reaches the set temperature and can hold it for a longer time. Regulation Tests if the sample block is controlled properly. for gradient-capable models only The Biometra TAdvanced software will automatically recommend at regular intervals to carry out an extended self-test.
- Page 106 Tools Biometra TAdvanced Fig. 96 Extended self-test screen The software provides a progress overview during the extended self-test. When a test has finished, the result P or F is displayed. For the currently running test, the ASSED AILED status R is shown and all other tests for which no result is shown are not UNNING finished yet.
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Page 107: Service Info File (Sinf)
Biometra TAdvanced Tools Fig. 98 Extended self-test overview screen (Biometra TAdvanced Twin) The results of the extended self-test are summarized in a log file, stored by the device and can be viewed by the user (see section "Extended self-test log file" p. 103). 11.5 Service info file (SINF) The service info file is a helpful tool for remote fault diagnosis by the Biometra service department. -
Page 108: Backup
Tools Biometra TAdvanced The service info file can be sent by e-mail to the Biometra service department. For contact details, refer to the inside front cover. 11.6 Backup The backup function is used to synchronize the memory contents between Biometra TAdvanced thermal cyclers. -
Page 109: Cycler Information
Biometra TAdvanced Tools 11.7 Cycler Information The Cycler Info screen provides general information about the device like: Cycler type Revision number power board Company Software version power board Serial number cycler Software version power board logic ... -
Page 110: Contact
Tools Biometra TAdvanced 11.8 Contact Shows contact details for the Biometra service department. Fig. 102 Service department contact details screen Version 02_20... -
Page 111: Adaptation Of Programs
Biometra TAdvanced Adaptation of programs Adaptation of programs If programs are exchanged between different thermal cycler models or the sample block is exchanged, programs might need to be adapted to the installed sample block type prior to the start or during editing. The following table provides an overview of possible program adaptations: Cause Example... -
Page 112: Quick Reference Guide
Quick reference guide Biometra TAdvanced Quick reference guide WARNING Before initial operation check the correct operating voltage is set on the voltage selection switch on the underside of the device. Do not open the housing of the device unless you are authorized to do so. Danger of electric shock! CAUTION The sample block and the heated lid can reach high temperatures during operation. - Page 113 Biometra TAdvanced Quick reference guide Home screen for Biometra TAdvanced Mono for Biometra TAdvanced Twin Press [P ] on the home screen to open the program overview: Editing, saving and ROGRAMS copying programs To edit a template, press [N ], select a template from the list and EW FROM TEMPLATE press [O...
- Page 114 Quick reference guide Biometra TAdvanced Use the cursor keys above the keyboard to switch between program steps. The number of the currently activated step is displayed on a blue background in the table at the top of the screen. ...
- Page 115 Biometra TAdvanced Quick reference guide To delete a program step, select it and press [D ELETE Incubation at 15 °C rather than 4 °C at the end of a run increases the device's life time. When all the parameters for all steps have been entered, confirm the settings on the programming screen with [D ...
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Page 116: Fault Removal
Fault removal Biometra TAdvanced Fault removal 14.1 Administrator password forgotten If the administrator has forgotten his password, use the F function (see ACTORY SETTINGS section "Factory settings" p. 93) to reset the system and download a backup file for recovery (see section "Backup" p. 108). 14.2 Slow heating and cooling The Biometra TAdvanced is equipped with strong fans that remove the heat from the heat sink. -
Page 117: Adaptation Of Programs From Other Thermal Cyclers
Biometra TAdvanced Fault removal 14.5 Adaptation of programs from other thermal cyclers Since the Biometra TAdvanced thermal cycler is a very fast device, it may be necessary to reduce the heating and cooling ramp when running protocols from other (slower) thermal cyclers (see section "Adjusting the ramp rate"... -
Page 118: Recommendations For The Use Of 384 Well Sample Blocks
Fault removal Biometra TAdvanced 14.7 Recommendations for the use of 384 well sample blocks Use the recommended 384 well plates and sealing materials To prevent sample evaporation by insufficient sealing use 384 well plates and sealing materials tested and recommended by Biometra. A list of plates and sealing is available at http://analytik-jena.com/. -
Page 119: Maintenance And Care
Biometra TAdvanced Maintenance and care Maintenance and care The Biometra TAdvanced is mainly maintenance-free. The care and maintenance tasks which can be performed by the customer are limited to the cleaning and disinfection of the housing and sample block. All maintenance work and repairs beyond this scope must only be performed by Biometra GmbH customer service personnel or authorized and trained persons. -
Page 120: Disinfecting The Device
Maintenance and care Biometra TAdvanced 15.2 Disinfecting the device WARNING Biological hazard! Clean the Biometra TAdvanced with particular care after analyzing potentially infectious material. Wear suitable protective equipment, e.g., protective gloves. NOTICE The only suitable cleaning method for the housing is wipe disinfection. When using spray disinfectants there is a risk that the liquid enters the sensitive electronic system through the ventilation slits. -
Page 121: Transport
Biometra TAdvanced Transport Transport If you have any problems with the device, please contact the customer service department or your local Biometra dealer. For the address of the Biometra customer service department refer to the inside front cover of this operating manual. Please observe the return information (see section "Device return information"... -
Page 122: Packing The Biometra Tadvanced
Transport Biometra TAdvanced 16.2 Packing the Biometra TAdvanced Biometra uses an extra designed packaging system with inserts of foamed material. NOTICE The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions are followed. Biometra is not liable for transport damage due to incorrect packaging. -
Page 123: Disposal
Biometra TAdvanced Disposal Disposal The user of the Biometra TAdvanced must dispose of any waste materials that occur during measurements (sample materials) in accordance with statutory and local regulations. At the end of its service life, the Biometra TAdvanced and all its electronic components must be disposed of as electronic waste in accordance with valid regulations. -
Page 124: Specifications
Specifications Biometra TAdvanced Specifications 18.1 Model specific data There is a range of 12 models with monoblocks or twinblocks, with specific capacities and block materials as well as with and without gradient function. Order number 846-x-070-211 846-x-070-201 Model Biometra TAdvanced 96 Biometra TAdvanced 96 G Capacity 96 x 0.2 ml tubes, 96 well micro plates or 12 x 8 well strips... - Page 125 Biometra TAdvanced Specifications Order number 846-x-070-251 846-x-070-241 Model Biometra TAdvanced 96 S Biometra TAdvanced 96 SG Capacity 96 x 0.2 ml tubes, 96 well micro plates or 12 x 8 well strips Block material Silver Block coating Gold Block exchange (optional) Quick-X-change Block exchange time less than 10 sec...
- Page 126 Specifications Biometra TAdvanced Order number 846-x-070-210 846-x-070-200 Model Biometra TAdvanced 60 Biometra TAdvanced 60 G Capacity 60 x 0.5 ml tubes Block material Aluminum Block coating Special alloy (gold color.) Block exchange (optional) Quick-X-change Block exchange time less than 10 sec Max.
- Page 127 Biometra TAdvanced Specifications Order number 846-x-070-224 846-x-070-214 Model Biometra TAdvanced 384 Biometra TAdvanced 384 G Capacity 384 well micro plates Block material Aluminum Block coating Special alloy Block exchange (optional) Quick-X-change Block exchange time less than 10 sec Max. heating rate* 4.0 °C/sec Max.
- Page 128 Specifications Biometra TAdvanced Order number 846-x-070-212 846-x-070-202 Model Biometra TAdvanced Twin 48 Biometra TAdvanced Twin 48 G Capacity Two blocks with 2 x 48 x 0.2 ml tubes, 2 x 48 well micro plates or 2 x 6 x 8 well strips Block material Aluminum Block coating...
- Page 129 Biometra TAdvanced Specifications Order number 846-x-070-213 846-x-070-215 Model Biometra TAdvanced Twin 30 Biometra TAdvanced Twin combi Capacity Two blocks with Two blocks with 2 x 48 x 0.2 ml tubes, 2 x 30 x 0.5 ml tubes 2 x 48 well micro plates 2 x 6 x 8 well strips or 2 x 35 x 0.5 ml tubes** Block material...
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Page 130: Specifications For All Models
Specifications Biometra TAdvanced 18.2 Specifications for all models The following specifications apply to all models: Software User-specific quick start option for the five most recent programs; program preview before start; option for toggling between spreadsheet and graphical programming mode; Linear Gradient Tool , service info file (SINF) generation;... -
Page 131: Declaration Of Conformity
Biometra TAdvanced Declaration of conformity Declaration of conformity Version 02_20... -
Page 132: Index
Index Biometra TAdvanced Index Auto restart 116 Heated lid temperature 47 Backup 108 Loop 50 Block module exchange 23 Name 47 Block status 36 New 45 Care 119 Pause 73 Cleaning the housing 119 Preheating 48 Configuration 85 Ramp rate 52 Declaration of conformity 131 Save 60 Decontamination 120...
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