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Troubleshooting
This section assumes working with Agilent MassHunter GCMS Acquisition 10.1 and above.
Performance
The method states "The response of the MRM product ion shall not vary by more than ±20%
throughout the run."
Upper limit = average of all PFTBA signal data points within method specified time range
multiplied by 1.2
Lower limit = average of all PFTBA signal data points within method specified time range
multiplied by 0.8
Symptom
Reference compound response not
stable
Reference compound signal
trending up during run
Reference compound signal
trending down during run
No reference compound signal but
other compounds present
G7050A - Reference Compound Introduction Valve
Possible Cause
Interference from matrix
Column bleed interference
Valve needs purging
Flow rate changing during run
Ambient temperature changes
at the vial can effect evaporation
of PFTBA.
Column bleed interference
Ambient temperature changes
at the vial can effect evaporation
of PFTBA.
Reference mass compound vial
needs refilling
Valve not opening
Cables are unplugged
Valve is leaking
Possible solution
Perform further sample cleanup
Inject less sample
Use column, Agilent DB-5, 60 m × 0.25 mm, 0.1 um (p/n 122-5061),
described in SGS AXYS Method 16130 and Agilent application notes,.
Column film thickness can affect the amount of bleed which intern
effects the stability of the reference mass response.
Purge valve before running
Use constant flow. Ensure column is configured correctly
Maintain stable temperature at reference valve vial location.
Use column described in SGS AXYS Method 16130 and Agilent
application notes, column film thickness can affect the amount of
bleed which intern effects the stability of the reference mass response.
Maintain stable temperature at reference valve vial location.
Refer to maintenance section of this manual
Manually open/close valve in the GC method editor screen and listen
for click of valve opening
If GC was moved away from the MS the valve cable may have been
unplugged. Check connection.
To troubleshoot used manual tune to review PFTBA in MS1 scan mode
or find air duster/similar masses. The valve can be opened and closed
via the GC front panel while in Manual tune screen
35
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