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CORNING MicroDEN Instruction Manual

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®
Corning
MicroDEN
Instruction Manual
Fully assembled Corning MicroDEN platform
®
Platform
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Summary of Contents for CORNING MicroDEN

  • Page 1 L A T I N A M E R I C A t 886 2-2716-0338 grupoLA@corning.com f 886 2-2516-7500 Brasil t 55 (11) 3089-7400 Mexico t (52-81) 8158-8400 Fully assembled Corning MicroDEN platform For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners.

  • Page 2: Table Of Contents

    ◗ ◗ 4. Setting up the Corning MicroDEN Unit ......4 Do not place any objects on top of this unit, and never stand or lean on the ◗...

  • Page 3: Introduction

    The MicroDEN unit is designed to be used on a laboratory benchtop, powered Two Corning 150 mL easy grip polystyrene storage bottles with dip tubes by the supplied external power supply. Up to 4 MicroDEN units may be placed ◗ –...

  • Page 4: Setting Up The Corning Microden Unit

    MOs into 2 single-use flasks followed by a medium refresh on Day 3 and dendritic cell harvest on Day 6. Duration may be increased or Cells can be seeded into the Corning MicroDEN flasks by either (Method 1) decreased depending on preference. Perfusion volume requirements should be positive pressure using a pipettor or (Method 2) pump perfusion.
  • Page 5 Outlet ports 3. Clamp the tubing onto the pumps by pressing down on the pump head clamp. Inlet ports 4. Turn the Corning® MicroDEN® unit “ON,” and select “Seed” using the function Thermoform button on the unit. tray 5. The pumps will perfuse the cell solution from the seeding bottle into the flasks.

  • Page 6: Differentiation Medium Loading

    1. Turn the Corning MicroDEN unit “OFF” and disconnect the power cord. 2. Transfer the MicroDEN unit and flasks that were set-up from the biosafety After seeding, a small air gap will remain at the top of the flask. The air gap is ◗...

  • Page 7: Harvest (Day 6)

    ◗ – in place so the tubes don’t twist (Figure 1). Take care not to touch the dip 5. Connect the power cord, turn the MicroDEN unit “ON”, and select “Harvest” tubes to any non-sterile surface. using the function button.
  • Page 8 Alternatively, the Figure 6. Slide the flasks side-to-side as medium drains into the harvest bottle. flasks or consumable kit can be gently vortexed. Do not place the MicroDEN unit on the vortex.

  • Page 9: Cleaning And Care

    Switch the tubing orientation so the tubing ◗ ◗ coming from the left side inlet bottle enters the Corning MicroDEN power requirements: 24 VDC ± 10%, 0.9 A (supplied by the ◗ ◗ left side of the pump. The colored marker on the...

  • Page 10: Limited Warranty

    4 July 2012 on waste electrical and electronic equipment (WEEE), the Corning® purchase. CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED MicroDEN® unit is marked with the crossed-out wheeled bin and must not be OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR disposed of with domestic waste.

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