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Problem
Cause
The pipettor
incorrectly
aspirates
The shaft surface is
the liquid
damaged or contami-
or the liquid
nated at the sealing site.
drops out
from the tip.
The plunger or the
O-ring is damaged due
to prolonged aspiration
The pipettor
of corrosive liquids.
incorrectly
aspirates
the liquid
The inside of the pipet-
tor is contaminated.
or the liquid
drops out
from the tip.
The sealing elements
are not sufficiently
lubricated.
The inside of the pipet-
tor is contaminated due
to aspiration of corrosive
substances.
Uneven work
of the pipet-
The inside of the pipet-
ting set, the
tor is contaminated due
pipetting
to the liquid entering the
pushbut-
pipettor.
ton gets
The sealing elements
blocked.
are not sufficiently
lubricated, e.g. after
repeated autoclaving
procedures.
Liquid with properties
other than water
(density, viscosity).
Incorrect
aspiration.
Tips with a filter
with increased flow
resistance.
If the problem persists after carrying out the above
steps, contact our regional representative.
Before returning the pipettor, please ensure the pipettor
is completely free of any chemical, radioactive, or micro-
biological contamination which could pose a threat
during transport and repair.
24 – VWR EHP PIPETTORS
Solution
Clean the shaft or
replace it with a new
one.
Disassemble the
shaft set; wash the
shaft, the plunger
and the seal (Section
10: Cleaning).
Replace the ele-
ments with new ones
if necessary. Apply
a small amount of
lubricant onto the
plunger and reas-
semble the set in the
correct order.
Unscrew the shaft
set, wash the parts.
Replace the ele-
ments with new ones
if necessary. Apply
a small amount of
lubricant on the
plunger and reas-
semble the set in the
correct order.
Calibrate the
pipettor using the
liquid which is to be
pipetted.
Calibrate the pipet-
tor using the tips
which are to be used
for pipetting.
12. Spare Parts
Contact our Customer Service to inquire about avail-
ability of spare parts. Pipettor model and name of the
part required should be specified. The most common
parts are depicted below.
NOTE: The replacement of the plunger requires conduct-
ing the calibration procedure according to Section 9.
Fixed Volume
A
N
F
G
J*
I
C
D
*Seal (J) used only in models 2, 5,000 and 10,000 µL
Model
Item
Description
Variable
Volume
B
adjustment
knob set
C,
Shaft
50, 100
I,
with O-ring
200, 250
J*
and seal
1,000
5,000
10,000
Variable Volume
A
N
F
G
J*
I
C
D
L
Model
Fixed
Cat. No.
All
-
SP29327
2
-
SP19131
10
5, 10
SP19132
20
20, 25
SP19133
50, 100
SP19134
200
SP19135
250-1,000
SP19136
-
SP19138
-
SP19139
Instruction Manual – 25
Qty
/Pk
1
1
1
1
1
1
1
1
1
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