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Variable Volume
• Set the preset volume depending on the pipettor
volume according to the following table.
Nominal
Preset
Permissible
volume
volume
values
(µL)
(μL)
Single-channel Pipettors
2
0.2
0.176 - 0.224
10
1
0.975 - 1.025
20
2
1.94 - 2.06
50
5
4.875 - 5.125
100
10
9.84 - 10.16
200
20
19.76 - 20.24
250
50
49.5 - 50.5
1,000
100
98.4 - 101.6
5,000
500
494 - 506
10,000
1,000
975 - 1,025
8-channel and 12-channel Pipettors
10
1
0.92 - 1.08
50
5
4.8 - 5.2
200
20
19.4 - 20.6
300
50
49.2 - 50.8
• Perform 10 aspirations, and calculate the average
value in (mg).
• Calculate the volume in (μL) by multiplying the value
in (mg) by the conversion factor Z (μL/mg).
If the average aspirated volume exceeds the permissible
values of the range, the pipettor should be recalibrated.
20 – VWR EHP PIPETTORS
Volume Change ΔV
(μL) for a Turn of
a Calibration Key by
1
(μL)
Turn
Increment
0.06
0.0025
0.33
0.63
0.0262
2.50
6.30
25.00
125.00
250.00
0.33
1.67
6.30
10.00
Pipettor Recalibration
1
1
0.0137
1. Remove the pipetting pushbutton.
0.104
CAUTION: In the variable pipettors the pipetting
pushbutton consists of 2 parts: the knob and the
pushbutton. After removal of the pushbutton, both
0.262
parts are separated.
2. Insert the calibration key into the calibration screw.
1.04
CAUTION: In variable pipettors hold the volume
5.2
setting knob to prevent rotation.
10.4
3. Turn the key clockwise to reduce the aspirated
volume, or counter-clockwise to increase the aspi-
rated volume. Use the values given in the above table
0.0137
to precisely adjust the volume.
0.070
4. Remove the key and replace the pipetting pushbutton
by first replacing the knob, then the pushbutton.
0.262
Determine the average aspirated volume. The average
0.42
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties consid-
erably different from those of water, follow the guidance
given in Section 5.
More information on the calibration procedure can be
found at www.vwr.com
2
3
K
Instruction Manual – 21
4
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