Metrohm 940 Professional IC Vario Series Manual
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Metrohm 940 Professional IC Vario Series Manual

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940 Professional IC Vario
940 Professional IC Vario ONE/SeS/Prep 1
Manual
8.940.8013EN / 2017-07-31

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Summary of Contents for Metrohm 940 Professional IC Vario Series

  • Page 1 940 Professional IC Vario 940 Professional IC Vario ONE/SeS/Prep 1 Manual 8.940.8013EN / 2017-07-31...
  • Page 3 Metrohm AG CH-9100 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 940 Professional IC Vario 940 Professional IC Vario ONE/SeS/ Prep 1 2.940.1410 Manual 8.940.8013EN / 2017-07-31...
  • Page 4 Technical Communication Metrohm AG CH-9100 Herisau techcom@metrohm.com This documentation is protected by copyright. All rights reserved. This documentation has been prepared with great care. However, errors can never be entirely ruled out. Please send comments regarding possible errors to the address above.

  • Page 5: Table Of Contents

    Installing the high-pressure pump ........26 3.10 Installing an inline filter ............. 27 3.11 Installing the pulsation absorber ........27 3.12 Injection valve ..............28 3.13 Metrohm Suppressor Module (MSM) ....... 30 3.13.1 Inserting the rotors ..............31 ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)
  • Page 6 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 3.13.2 Connecting the Metrohm Suppressor Module (MSM) ..... 34 3.14 Metrohm CO Suppressor (MCS) ........38 3.14.1 General information on the MCS ........... 38 3.14.2 Connecting the MCS ............. 39 3.14.3 Installing adsorption cartridges ..........40 3.15 Installing the conductivity detector ........
  • Page 7 Notes for operating the Metrohm Suppressor Module (MSM) ................... 87 5.12.2 Taking care of the suppressor housing ........88 5.12.3 Servicing the Metrohm Suppressor Module (MSM) ....88 5.13 Servicing the Metrohm CO Suppressor (MCS) ....96 5.13.1 Replacing the CO adsorption cartridge CW ......
  • Page 8 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 7.17 Interfaces ................113 8 Accessories Index ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)
  • Page 9 Figure 11 Pulsation absorber ................28 Figure 12 Exchanging the sample loop ............29 Figure 13 Metrohm Suppressor Module (MSM) – Connection capillaries ..34 Figure 14 Connecting the MCS ............... 39 Figure 15 Peristaltic pump ................50 Figure 16 Column thermostat .................

  • Page 11: Introduction

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction Instrument description The 940 Professional IC Vario is a professional ion chromatograph. It is dis- tinguished by: Its intelligence: All of the functions are monitored, optimized and docu- ￭ mented in an FDA-compatible manner. Intelligent components, such as iColumns, save important data onto a chip.
  • Page 12 Metrohm Suppressor Module (MSM) The chemical suppressor MSM consists of the suppressor drive, a rotor and, where applicable, an adapter. The suppressor drive gives you the flex- ibility to use different rotors according to the principle "one drive –...

  • Page 13: Intended Use

    This lowers the background conductivity, improves detection sen- sitivity and minimizes the injection peak and system peak. Detector Metrohm offers a series of different detectors for various analysis tasks. A suitable detector type must be ordered as a separate device. Sample degasser The sample degasser removes gas bubbles and dissolved gases from the sample.

  • Page 14: Safety Instructions

    The electrical safety when working with the instrument is ensured as part of the international standard IEC 61010. WARNING Only personnel qualified by Metrohm are authorized to carry out service work on electronic components. WARNING Never open the housing of the instrument. The instrument could be damaged by this.

  • Page 15: Tubing And Capillary Connections

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Protection against electrostatic charges WARNING Electronic components are sensitive to electrostatic charges and can be destroyed by discharges. Do not fail to pull the power cord out of the power socket before you set up or disconnect electrical plug connections at the rear of the instrument.

  • Page 16: Symbols And Conventions

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Symbols and conventions More details about the disposal of your old instrument can be obtained from your local authorities, from waste disposal companies or from your local dealer. Symbols and conventions The following symbols and formatting may appear in this documentation: Cross-reference to figure legend The first number refers to the figure number, the sec- ond to the instrument part in the figure.

  • Page 17: Overview Of The Instrument

    Offers space for the eluent bottle(s) and Offers space for two embedded detectors additional accessories. and additional accessories. Column holder Metrohm CO Suppressor (MCS) For a third separation column outside the column thermostat. ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)
  • Page 18 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.1 Front Metrohm Suppressor Module (MSM) Column thermostat With two column holders for two separation columns. Inline filter Sample degasser Injection valve 10 Pulsation absorber 11 Holder 12 Base tray For a dialysis cell or ultrafiltration cell. With leak sensor.

  • Page 19: Rear

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument Rear Figure 2 Rear Bottle holder Back panel Offers space for the eluent bottle(s) and Removable. Enables access to the detector additional accessories. chamber. Drainage tubing connection Transport locking screws For connecting the drainage tubing, which For securing the vacuum pump(s) when guides escaped liquids away from the detec- transporting the instrument.

  • Page 20: Feed-Throughs For Capillaries And Cables

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Feed-throughs for capillaries and cables Vacuum connection Exhaust opening For connecting an Extension Module that Labeled Exhaust. For extracting the air from has a degasser but not its own vacuum the vacuum chamber. pump. This connection has to be firmly sealed with a stopper when not in use.

  • Page 21: Figure 3 Feed-Throughs On The Door

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument Openings on the door Figure 3 Feed-throughs on the door Luer connector Opening for capillaries For connecting a capillary from inside and For up to 3 capillaries. for inserting a syringe (6.2816.020) from outside. For manual sample injection. An opening for up to 3 capillaries is located on the door of the instru- ment.

  • Page 22: Figure 4 Openings For Capillaries And Cables

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Feed-throughs for capillaries and cables Openings on the back panel Figure 4 Openings for capillaries and cables Openings for capillaries Openings for cables The removable back panel is outfitted with openings through which capil- laries and cables can be lead out of the detector chamber. Ducts for capillaries There are ducts for capillaries between the instrument and base tray as well as between the instrument and the bottle holder.

  • Page 23: Figure 5 Ducts For Capillaries

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument Figure 5 Ducts for capillaries ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)

  • Page 24: Installation

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.1 Setting up the instrument 3 Installation Setting up the instrument 3.1.1 Packaging The instrument is supplied in protective packaging together with the sepa- rately packed accessories. Keep this packaging, as only this ensures safe transportation of the instrument. 3.1.2 Checks Immediately after receipt, check whether the shipment has arrived com-...
  • Page 25 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Also see: PEEK pressure screws 1x1 video on the Internet http://ic- help.metrohm.com. Connection capillaries PEEK capillaries and PTFE capillaries are used in the IC system. PEEK capillaries (poly- PEEK capillaries are pressure-stable up to 400 bar (depending on the inner etheretherketone) diameter), flexible, chemically inert and have an extremely smooth surface.
  • Page 26 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Capillary connections in the IC system occurs if two capillary ends connected to each other do not fit exactly, thus allowing liquid to escape. There are two possible causes for this: The capillary ends do not have exactly flat edges. ￭...

  • Page 27: Removing The Handle

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Removing the handle The instrument is equipped with a handle in order to make it easier to transport. The handle can be removed once the instrument is in place in the lab. Accessories You do not need any accessories for the following work steps. Removing the handle 1 Removing the handle Unscrew the four knurled screws.

  • Page 28: Removing Transport Locking Screws

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.4 Removing transport locking screws Removing transport locking screws To avoid damage to the drives for the high-pressure pump and the vac- uum pump during transport, the pumps are secured with transport locking screws. These are located at the rear of the instrument and labeled with Transport security screws.

  • Page 29: Connecting The Drainage Tubing And Leak Sensor

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Store the transport locking screws in a safe place. Reinsert the transport locking screws each time you transport the instrument a significant dis- tance. CAUTION The pumps may be damaged if you transport the instrument without inserting the transport locking screws. Connecting the drainage tubing and leak sensor The leak sensor detects leaking liquid that collects in the instrument's base tray.
  • Page 30 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.5 Connecting the drainage tubing and leak sensor Connecting the drainage tubing 1 Cut a piece of silicone tubing into three pieces using scissors: 2 × approx. 40 cm and 1 × 20 cm. 2 Attach one end of the 40 cm long piece to the drainage tubing con- nection on the bottle holder.

  • Page 31: Connecting The Leak Sensor

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Route the loose end into a waste container. 3.5.2 Connecting the leak sensor Plugging in the leak sensor connection cable The leak sensor connection cable is coiled up in the base tray. 1 Pull the leak sensor connection cable out of the base tray as far as needed.

  • Page 32: Connecting The Eluent Bottle

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 Connecting the eluent bottle Connecting the eluent bottle The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing . The eluent aspiration tubing is installed on the entry to the eluent degasser. The tubing must be be fed out of the instrument through a suitable open- ing (see "Openings on the back panel", page 12) before the loose end can be connected to the eluent bottle.
  • Page 33 (6.2744.360), a syringe and the purge needle (6.2816.040). This procedure can also be found in the video "Inserting a new aspi- ration filter" at the following link: ic-help.metrohm.com 4 Mounting aspiration filter NOTE Always wear gloves when handling the aspiration filter.

  • Page 34: Figure 8 Installing Tubing Weighting And Aspiration Filter

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 Connecting the eluent bottle Figure 8 Installing tubing weighting and aspiration filter 5 Installing the eluent bottle cap on the eluent bottle Insert the eluent aspiration tubing into the eluent bottle ￭ (6.1608.070). Tighten the bottle cap on the eluent bottle. ￭...
  • Page 35 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 6 Mounting the adsorber tube NOTE Depending on the eluent used, the adsorber tube (6.1609.000) must be filled differently: For alkaline eluents or eluents with a low buffer capacity: first a ￭ little cotton, then with CO adsorber material.

  • Page 36: Connecting The Eluent Degasser

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Connecting the eluent degasser Connecting the eluent degasser The high-pressure pump cannot generate uniform flow if the eluent con- tains small gas bubbles or dissolved gas. As a result, the baseline cannot be stabilized correctly. In order to achieve good measurement results, the eluent has to be degassed before it goes into the high-pressure pump.

  • Page 37: Installing An Inline Filter

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.10 Installing an inline filter Inline filters protect the separation column reliably from potential contami- nation from the eluent. The small filter pads with 2 µm pore size can be replaced quickly and easily. They remove particles from the solutions. An inline filter (6.2821.120) is installed between the purge valve and the pulsation absorber as protection against particles.

  • Page 38: Injection Valve

    The quantity of sample solution injected is determined by: the volume of the sample loop or ￭ by an 800 Dosino when the Metrohm intelligent Partial Loop Injection ￭ Technique (MiPT), the Metrohm intelligent Pick-up Injection Technique (MiPuT) or the Metrohm Inline Preconcentration (MiPCT, MiPCT-ME) is used.

  • Page 39: Figure 12 Exchanging The Sample Loop

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Application Sample loop MiPCT, MiPCT-ME Preconcentration column The injection valve is completely connected. No installation work is required. Optional: Exchanging the sample loop The sample loop can be replaced to match the application (see Table 1, page 28). NOTE Only use PEEK pressure screws (6.2744.010) to connect capillaries and the sample loop to the injection valve.

  • Page 40: Metrohm Suppressor Module (Msm)

    Rotor A (6.2844.000), must first be fitted into the adapter (6.2842.020), which can then be inserted into the suppressor housing. A connecting piece (6.2835.010) is used for all rotors for connecting the Metrohm Suppressor Module (MSM) to the IC system. ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)

  • Page 41: Inserting The Rotors

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 6.2842.020 6.2842.020 6.2844.000 6.2832.000 6.2842.000 6.2835.000 6.2842.200 NOTE The instruments are supplied without rotor and without adapter. The suitable rotor and the adapter, if required, must be ordered sepa- rately. 3.13.1 Inserting the rotors Accessories For this step, you need the following accessories: For suppression: MSM Rotor A (6.2832.000) or MSM‑HC Rotor A ￭...
  • Page 42 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Metrohm Suppressor Module (MSM) Inserting large rotors 4 mm 6.2842.000 6.2842.200 6.2835.010 1 Removing the union nut Loosen the union nut and remove it. 2 Inserting the rotor Clean the sealing surface of the rotor with ethanol using a lint- ￭...
  • Page 43 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Inserting the connecting piece Clean the sealing surface of the connecting piece with ethanol ￭ using a lint-free cloth. Insert the connecting piece into the suppressor drive so that con- ￭ nector 1 is on top and the three pins of the connecting piece fit into the corresponding recesses on the suppressor drive.

  • Page 44: Connecting The Metrohm Suppressor Module (Msm)

    The three entries and exits of the suppressor units, numbered 1, 2 and 3 on the connecting piece, each have two permanently installed PTFE capil- laries. Figure 13 Metrohm Suppressor Module (MSM) – Connection capillaries Outlet capillary for the eluent. Inlet capillary for the eluent. regenerant waste reg.
  • Page 45 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Recommended installation Alternative installation 3.13.2.1 Installing the eluent path The eluent path is connected with the capillaries in and out. 1 Connect the capillary labeled in to the outlet of the separation col- umn using a short pressure screw (6.2744.070). 2 Use a long pressure screw (6.2744.090) to connect the capillary labeled out to the inlet of the MCS (see Chapter 3.14.2, page 39).
  • Page 46 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Metrohm Suppressor Module (MSM) 3.13.2.3 Connecting the regeneration solution The regeneration solution is connected to the regenerant capillary. Connecting the regeneration solution to a Dosino For this step, you need the following accessories: Dosino (2.800.0010) ￭ Accessory kit: Flex/Vario: SeS (6.5000.020) ￭...
  • Page 47 4 Connect the FEP tubing (6.1805.120) from the bottle with the regen- eration solution to Port 2 of the Dosino. 3.13.2.4 Connecting the rinsing solution Various possibilities exist for rinsing the Metrohm Suppressor Module: Rinsing solution via STREAM (recommended) ￭ Use the eluent from the conductivity detector as rinsing solution.

  • Page 48: Metrohm Co Suppressor (Mcs)

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Metrohm CO Suppressor (MCS) Inline filter (6.2821.120) ￭ PTFE capillary (6.1803.030) ￭ Adapter M6/UNF (6.2744.080) ￭ FEP tubing M6 / 100 cm (6.1805.120) ￭ Adapter Dosino Port 4, M6 inner (6.1808.280) ￭ 1 Fasten the capillary labeled rinsing solution to the outlet of an inline filter (6.2821.120) using a pressure screw (6.2744.070).

  • Page 49: Connecting The Mcs

    CO adsorption cartridge to filter out the CO from the air. 3.14.2 Connecting the MCS The MCS is connected between the Metrohm Suppressor Module (MSM) and the conductivity detector. Connecting the MCS Figure 14 Connecting the MCS Air aspiration capillary Pressure screw, short (6.2744.070)

  • Page 50: Installing Adsorption Cartridges

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Metrohm CO Suppressor (MCS) 2 Connection from the suppressor Use a long pressure screw (6.2744.090) to connect the capillary of the MSM labeled out to the input of the MCS (labeled In). 3 Connection to the detector Connect the inlet capillary of the conductivity detector with a long pressure screw (6.2744.090) to the MCS output (labeled Out).
  • Page 51 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Remove the label from the lid of the CO adsorption cartridge CW. This uncovers the small opening in the lid of the CO adsorption car- tridge CW, through which air is aspirated. The CO adsorption cartridge CW is now ready for installation. NOTE adsorption cartridge CW (6.2837.100) works without The new CO...
  • Page 52 Push two clips into the slots on the adsorption cartridge holder. ￭ 2 Connecting the CO adsorption cartridge CW Attach the capillary connected to the Metrohm CO Suppressor's (MCS) Air in connection to the tip of the CO adsorption cartridge...

  • Page 53: Installing The Conductivity Detector

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 4 Putting the adsorption cartridge holder into the instrument Place the adsorption cartridge holder with the CO adsorption car- ￭ tridge CW into the instrument's detector chamber. 3.15 Installing the conductivity detector The 940 Professional IC Vario provides enough space for two detectors and additional accessories in the detector chamber.

  • Page 54: Installing The Amperometric Detector

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16 Installing the amperometric detector 1 Tighten the detector inlet capillary to the Out outlet of the MCS using a long pressure screw (6.2744.090). 3.16 Installing the amperometric detector The 940 Professional IC Vario provides enough space for two detectors and additional accessories in the detector chamber.
  • Page 55 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the sample degasser CAUTION If the sample degasser is not used, the inlet and outlet must be sealed with threaded stoppers (6.2744.220). 1 Removing the threaded stoppers Remove and keep the threaded stoppers (6.2744.220) from the inlet and outlet of the sample degasser.

  • Page 56: Peristaltic Pump

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 Peristaltic pump Guide the other end of the PTFE capillary (6.1803.040) out of the ￭ instrument through a capillary feed-through and connect it to the Sample Processor, if applicable. Try to ensure the shortest possible connections (shorten the capil- lary if necessary).
  • Page 57 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 2 Select an adapter suitable for the pump tubing. The adapters are included with the pump tubing connection with locking nut and filter (6.2744.180). Table 3 Pump tubing and suitable adapters Pump tubing Adapter 6.1826.310 (orange/green) 6.1826.320 (orange/yellow) 6.1826.330 (orange/white) 6.1826.340 (black/black) 6.1826.360 (white/white)
  • Page 58 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 Peristaltic pump 1 Connecting the pump tubing Attach the coupling olive/UNF 10/32 (6.2744.034) to the pump ￭ tubing entry. Push the end of the pump tubing over at least the second notch of the olive so that the pump tubing is firmly in place.
  • Page 59 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Inserting the pump tubing Press the tubing cartridge's contact pressure lever down all the ￭ way. Place the pump tubing in the tubing cartridge. Fit the tubing car- ￭ tridge between two stoppers. The stoppers must snap into the corresponding holder of the tubing cartridge.

  • Page 60: Mode Of Operation For The Peristaltic Pump

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 Peristaltic pump When liquid starts flowing, raise the contact pressure lever by an ￭ additional two ratchet increments. The contact pressure is now set optimally. 3.18.2 Mode of operation for the peristaltic pump The peristaltic pump is used for pumping sample and auxiliary solutions. It can rotate in both directions.

  • Page 61: Connecting The Instrument To A Computer

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.19 Connecting the instrument to a computer NOTE If the instrument is connected to the computer, then it must be switched off. Accessories For this step, you need the following accessories: USB connecting cable (6.2151.020) ￭ Connecting the USB cable 1 Insert the USB cable into the computer connection socket on the rear of the instrument labeled PC.

  • Page 62: Connecting The Instrument To The Power Grid

    Unplug the power plug immediately if you suspect that moisture has ￭ gotten inside the instrument. Only personnel who have been issued Metrohm qualification may ￭ perform service and repair work on electrical and electronic parts. Connecting the power cord Accessories Power cord, three-core with IEC 60320 instrument plug type C13.

  • Page 63: Initial Start-Up

    Check whether the detector outlet capillary is connected to the Metrohm Suppressor Module (MSM)'s inlet capillary for rinsing solu- tion (labeled rinsing solution).
  • Page 64 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.21 Initial start-up 3 Starting equilibration Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equili- ￭ bration ▶ Start HW. 4 Deaerating the high-pressure pump Push the end of the purge needle (6.2816.040) over the end of ￭...

  • Page 65: Connecting And Rinsing The Guard Column

    Connecting and rinsing the guard column Guard columns protect separation columns and significantly increase their service life. The guard columns available from Metrohm are either actual guard columns or guard column cartridges used together with a cartridge holder. The process of installing a guard column cartridge into the corre- sponding holder is described in the guard column leaflet.
  • Page 66 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.22 Connecting and rinsing the guard column NOTE The guard column may not be connected until after the instrument has already been put into operation once (see Chapter 3.21, page 53). The guard column and the separation column have to be replaced by a cou- pling (6.2744.040) until then.

  • Page 67: Connecting The Separation Column

    NOTE Information regarding which separation column is suitable for your application can be found in the Metrohm Column Program, the product information for the separation column or it can be obtained through your representative.
  • Page 68 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.23 Connecting the separation column CAUTION New separation columns are filled with a solution and sealed with stop- pers on both sides. Before inserting the column, ensure that this solu- tion can be mixed with the eluent being used (follow the information provided by the manufacturer).
  • Page 69 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the separation column 1 Removing the stoppers Remove the stoppers from the separation column. ￭ ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)
  • Page 70 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.23 Connecting the separation column 2 Installing the inlet of the separation column CAUTION When inserting the column, ensure that it is inserted correctly based on the marked flow direction. There are three options: Attach the column inlet directly onto the guard column or, ￭...

  • Page 71: Conditioning

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.24 Conditioning In the following cases, the system must be conditioned with eluent until a stable baseline has been reached: After installation ￭ After each time the instrument is switched on ￭ After each eluent change ￭ NOTE The conditioning time can lengthen considerably if the composition of the eluent is modified.
  • Page 72 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.24 Conditioning Visually inspect whether all capillaries and their connections from ￭ the high-pressure pump to the detector are leak-tight. If eluent is leaking out anywhere, tighten the corresponding pressure screw further, or loosen the pressure screw, check the end of the capil- lary and shorten it using the capillary cutter if necessary and retighten the pressure screw.

  • Page 73: Operation

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation 4 Operation The 940 Professional IC Vario ONE/SeS/Prep 1 is operated solely using the MagIC Net software. You can find information on operating the software in the tutorial for MagIC Net or in the online help. ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)

  • Page 74: Operation And Maintenance

    Maintenance by Metrohm Service Maintenance of the instrument is best carried out as part of an annual service performed by specialist personnel from Metrohm. A shorter main- tenance interval is recommended if you frequently work with caustic and corrosive chemicals. Metrohm Service offers every form of technical advice for maintenance and service of all Metrohm instruments.

  • Page 75: Shutting Down And Recommissioning

    3 Rinse the IC system for 15 minutes with methanol/ultrapure water mixture (1:4). 4 Optional: Only if the IC system is equipped with a suppressor. In the software, switch the Metrohm Suppressor Module (MSM) twice during the rinsing process at five-minute intervals in each case (STEP command).

  • Page 76: Capillary Connections

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.2 Capillary connections Capillary connections All capillary connections between the injection valve, the separation col- umn and the detector must be as short as possible, have a low dead vol- ume and be completely leak-tight. The PEEK capillary downstream of the detector must be free of blockages. Use only PEEK capillaries with an inner diameter of 0.25 mm between the high-pressure pump and the detector (high-pressure section).

  • Page 77: Figure 16 Column Thermostat

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Figure 16 Column thermostat Openings Preheating grooves For feeding capillaries into and out of the For regulating the temperature of the eluent. instrument. Column holder With column recognition. For fastening the column. ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)

  • Page 78: Handling The Eluent

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 Handling the eluent Replacing the preheating capillaries In order to make threading the column inlet capillary into the column ther- mostat's preheating grooves easier, we recommend loosening the screws for the holder plate a little, but not removing them all the way. 1 Guide the column inlet capillary into the column thermostat via a suitable opening (16-1).

  • Page 79: Changing The Eluent

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance The composition of the eluent plays a critical role in chromatographic analysis: Concentration An increase in the concentration generally leads to shorter retention times and faster separation, but also to a higher background conductivity sig- nal.

  • Page 80: Notes On Operating The High-Pressure Pump

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.7 Notes on operating the high-pressure pump Connecting the connecting tubing Insert the clamping screws on the connector and screw them in ￭ by hand. Tighten the clamping screws with the wrench. ￭ Notes on operating the high-pressure pump CAUTION The pump head is filled ex works with methanol/ultrapure water.

  • Page 81: Servicing The High-Pressure Pump

    5 Operation and maintenance Servicing the high-pressure pump NOTE You can find a video sequence for this task in the Multimedia Guide IC Maintenance or on the Internet at http://ic-help.metrohm.com/. Figure 17 High-pressure pump – Parts Pressure screw, short (6.2744.070) Outlet valve holder Fastened to the outlet valve holder.
  • Page 82 Insert the piston. Reinstall the pump head. You can find brief video sequences on the following maintenance steps on the Internet at http://ic-help.metrohm.com/. Servicing the outlet valve and inlet valve Accessories For this step, you need the following accessories: You can find these parts in the accessory kit: Vario/Flex Basic (6.5000.000).
  • Page 83 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Cleaning the outlet valve Spare parts If the outlet valve cannot be cleaned, you will need a new outlet valve (6.2824.160) for this step. 1 Removing the outlet valve Unscrew the connection capillary to the auxiliary piston from the ￭...
  • Page 84 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump 3 Reinserting the outlet valve into the pump head Insert the outlet valve into the outlet valve holder (the seal must ￭ be visible) (1). Screw the outlet valve holder up into the pump head (2) and ￭...
  • Page 85 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Cleaning the inlet valve Spare parts If the inlet valve cannot be cleaned, you will need a new inlet valve (6.2824.170) for this step. 1 Removing the inlet valve Unscrew the connection capillary to the connection of the eluent ￭...
  • Page 86 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump 3 Reinserting the inlet valve into the pump head Insert the inlet valve into the inlet valve holder (the seal must be ￭ visible) (1). Screw the inlet valve holder up into the pump head (2) and ￭...
  • Page 87 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 1 Release the coupling from the pressure screw and seal it with a stop- per. 2 Unscrew and remove the pressure screw on the pump head's outlet (17-8). 3 Loosen and remove the four fastening screws (17-3) using the hex key.

  • Page 88: Figure 18 High-Pressure Pump - Cross-Section

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump Figure 18 High-pressure pump – Cross-section Outlet valve holder Outlet valve (6.2824.160) Backup ring Piston seal (6.2741.020) Pump head Inlet valve (6.2824.170) Inlet valve holder Zirconium oxide piston (6.2824.070) Piston cartridge Accessories For this step, you need the following accessories: You can find these parts in the accessory kit: Vario/Flex Basic (6.5000.000).

  • Page 89: Figure 19 Tool For Piston Seal (6.2617.010)

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Figure 19 Tool for piston seal (6.2617.010) Sleeve Spare part For this step, you need a new piston seal (6.2741.020). Replacing the piston seal Figure 20 Removing the piston cartridge from the pump head Piston cartridge Backup ring 1 Removing the piston cartridge Loosen the piston cartridge (20-1) using the adjustable wrench and...

  • Page 90: Figure 21 Inserting The Piston Seal Into The Tool

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump 3 Removing the old piston seal CAUTION Screwing the (6.2617.010) special tool for the piston seal into the piston seal destroys this completely! CAUTION Avoid touching the sealing surface in the pump head with the tool! Only screw the tip (19-1) of the tool for the piston seal far enough into the piston seal that the seal can be pulled out.

  • Page 91: Figure 22 Parts Of The Piston Cartridge

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance The pump head has been removed (see "Removing the pump head", ￭ page 76). The piston cartridge is removed (see "Replacing the piston seal", page ￭ 79). For this task, you need the following accessories: Zirconium oxide piston (6.2824.070) ￭...
  • Page 92 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump 2 Cleaning the parts of the piston cartridge If the zirconium oxide piston has become contaminated due to ￭ abrasion or deposits, then clean it using a fine abrasive cleaning powder, rinse it using ultrapure water until it is free of particles and dry it.
  • Page 93 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 4 mm hex key (6.2621.030) ￭ Mounting the pump head NOTE The pump head cannot be mounted the wrong way, as it has fastening bolts with different bore hole depths, i.e., one fastening bolt is longer than all others.

  • Page 94: Servicing The Inline Filter

    Servicing the inline filter NOTE You can find a video sequence for this task in the Multimedia Guide IC Maintenance or on the Internet at http://ic-help.metrohm.com/. Maintenance interval The filter must be replaced at least every 3 months; it may need to be replaced more frequently, depending on the application.

  • Page 95: Figure 23 Inline Filter - Removing The Filter

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Removing the filter Figure 23 Inline filter – Removing the filter Filter housing Filter screw Inline filter housing. Part of the accessories Screw for the inline filter. Part of the acces- (6.2821.120). sories (6.2821.120). Filter (6.2821.130) Pack contains 10 pieces.
  • Page 96 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.9 Servicing the inline filter Inserting a new filter 1 Inserting a new filter Use tweezers to carefully place a new filter into the filter screw ￭ (23-2) so that it is flat and press it firmly into place with the rear of the tweezers.

  • Page 97: Servicing The Pulsation Absorber

    If the Metrohm Suppressor Module (MSM) is in a dry state, it must be rinsed for at least five minutes before it may be switched over.

  • Page 98: Taking Care Of The Suppressor Housing

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Metrohm Suppressor Module (MSM) CAUTION The Metrohm Suppressor Module (MSM) must be regenerated (see Chapter 5.12.3.2, page 89), cleaned (see Chapter 5.12.3.4, page 91) or replaced (see Chapter 5.12.3.5, page 94) if the capacity of the Met- rohm Suppressor Module (MSM) is reduced or if the back pressure is high.
  • Page 99 Pump tubing made of PVC must not be used for solutions containing organic solvents. We recommend using the high-pressure pump for regeneration. Regenerating the anion suppressor rotor 1 Disconnecting the Metrohm Suppressor Module (MSM) from the IC system Disconnect the capillaries of the MSM labeled regenerant and ￭...
  • Page 100 As soon as all three suppressor units have been rinsed, disconnect ￭ the capillary labeled rinsing solution from the coupling. 4 Connecting the Metrohm Suppressor Module (MSM) to the IC system Reconnect the capillaries of the MSM labeled regenerant and ￭...
  • Page 101 Equilibrate the system as usual (see chapter "Conditioning" in the manual for the ion chromatograph). 5.12.3.4 Cleaning the Metrohm Suppressor Module (MSM) In the following cases, it may be necessary to clean the Metrohm Suppres- sor Module (MSM): Increased back pressure at the MSM's connection tubing. ￭ ￭￭￭￭￭￭￭￭...
  • Page 102 Check whether water comes out at the connecting piece. ￭ If one of the capillaries remains blocked, the connecting piece (see "Replacing parts of the Metrohm Suppressor Module (MSM)", page 94) must be replaced (order number 6.2835.010). 4 Cleaning the rotor Clean the sealing surface of the rotor (24-3) with ethanol using a ￭...
  • Page 103 Reattach the union nut (24-1) and tighten by hand (do not use a ￭ tool). 8 Connecting and conditioning the Metrohm Suppressor Mod- ule (MSM) Reconnect the MSM to the IC system. ￭...
  • Page 104 5.12 Metrohm Suppressor Module (MSM) 5.12.3.5 Replacing parts of the Metrohm Suppressor Module (MSM) Parts of the Metrohm Suppressor Module (MSM) may need to be replaced in the following cases: Irremediable loss of suppressor capacity (reduced phosphate sensitivity ￭ and/or significant rise in the baseline).
  • Page 105 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance NOTE The adapter (6.2842.020) (see "Inserting small rotors", page 33) is required in order to insert a small rotor into the suppressor drive. 4 mm Insert the new rotor (24-3) into the housing (24-4) in such a way ￭...

  • Page 106: Servicing The Metrohm Co 2 Suppressor (Mcs)

    CW 1 Remove the depleted CO adsorption cartridge CW from the adsorp- tion cartridge holder. 2 Remove the Air in capillary of the Metrohm CO Suppressor (MCS). 3 Professionally dispose of the depleted CO adsorption cartridge CW. Installing the new CO...

  • Page 107: Servicing The Detector

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 5.14 Servicing the detector Follow the maintenance instructions in the the detector's manual. 5.15 Rinsing the sample path Before a new sample is measured, the sample path must be rinsed with it long enough to prevent the measuring result from being falsified by the previous sample (sample carry-over).

  • Page 108: Peristaltic Pump

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.16 Peristaltic pump If the sample is injected automatically, the rinsing time must be at least three times the transfer time. Checking the rinsing time You can determine if the applied rinsing time is sufficient by measuring the sample carry-over directly. Proceed as follows to do this: 1 Preparing two samples Sample A: A typical sample for the application.

  • Page 109: Servicing The Peristaltic Pump

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance CAUTION The service life of the pump tubing primarily depends on the contact pressure. If the peristaltic pump is switched off for long periods, lift up the tubing cartridges on the right side by releasing the snap-action levers. This ensures that the contact pressure will be maintained once it has been set.

  • Page 110: Separation Column

    You can find detailed information on the separation columns available from Metrohm in the leaflet provided along with your separation column, in the Metrohm IC Column Program (available from your Metrohm representative) or on the Internet at http://www.metrohm.com...

  • Page 111: Protecting The Separation Column

    The separation column can be regenerated according to the column man- ufacturer's specifications if the separation characteristics of the column have deteriorated. You can find information on regenerating separation columns available from Metrohm on the leaflet provided with every col- umn. NOTE Regeneration is intended as a last resort.

  • Page 112: Quality Management And Qualification With Metrohm

    Metrohm offers you comprehensive support in implementing quality man- agement measures for instruments and software. Qualification Please contact your local Metrohm representative for support in qualifica- tion of instruments and software. The Installation Qualification (IQ) and Operational Qualification (OQ) are offered by Metrohm represen- tatives as a service.

  • Page 113: Troubleshooting

    The pulsation absorber is Connect (see Chapter 3.11, page 27) or not connected or is defec- replace the pulsation absorber. tive. Contact Metrohm Service. MCS – The vacuum pump is defective. The baseline is drift- Thermal equilibrium is not Condition the instrument with the column ing.
  • Page 114 Replace the separation column (see "Con- ￭ necting the separation column", page 59). Note: Samples should always be microfiltered . Injection valve – blocked. Have the valve cleaned (by a Metrohm service engineer). The retention times Eluent - Incorrect concen- Create eluent with correct concentration.
  • Page 115 Column chip is defective. 1. Save column configuration in MagIC Net. 2. Notify Metrohm Service. Individual peaks are Sample – Sample carry- Check the rinsing time (see "Checking the rins- greater than expec- over from previous meas- ing time", page 98).
  • Page 116 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem Cause Remedy The retention times The eluent path has a leak. Check all of the connections along the eluent are poorly reprodu- path and fix the leak. cible. The eluent path is blocked. Check the eluent path and eliminate the block- age.
  • Page 117 Sample – The rinsing vol- Increase the rinsing time (see Chapter 5.15, ume is too small. page 97). Injection valve – Defective. Request Metrohm Service. MCS – The vacuum is too Check the connectors. If they are OK: ￭ low.

  • Page 118: Technical Specifications

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.1 Reference conditions 7 Technical specifications Reference conditions The technical specifications listed in this chapter refer to the following ref- erence conditions: Ambient tempera- +25 °C (± 3 °C) ture Instrument status Operating > 40 minutes (in equilibrium) Instrument IC system Metal-free IC system ￭...

  • Page 119: Housing

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Technical specifications Housing Dimensions Width 365 mm Height 642 mm Depth 380 mm Controls LED standby indicator Indicators On/off switch On the rear of the instrument Weight 2.940.1410 35.30 kg (without accessories) Leak sensor Type Electronic, no calibration necessary Column thermostat Type Thermostat using Peltier technology for two intelligent separation col-...

  • Page 120: Eluent Degasser

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.8 Eluent degasser Eluent degasser Material Fluoropolymer Resistance to sol- No restriction (except PFC) vents Time to establish < 60 s vacuum High-pressure pump Type Serial dual-piston pump ￭ Intelligent pump head recognition ￭ Chemically inert ￭ Metal-free pump heads ￭...

  • Page 121: Injection Valve

    Injection valve Actuator switch- typ. 100 ms ing time Maximum operat- 35 MPa (350 bar) ing pressure Material PEEK 7.11 Metrohm Suppressor Module (MSM) No restriction Resistance to sol- vents Switching time typ. 100 ms 7.12 Metrohm CO Suppressor (MCS) Material...

  • Page 122: Detector

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.13 Detector 7.13 Detector You can find the technical specifications for the detector in the manual for the detector. 7.14 Sample degasser Material Fluoropolymer Resistance to sol- No restriction (except PFC) vents Time to establish < 60 s vacuum 7.15 Peristaltic pump Type...
  • Page 123 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Technical specifications 7.17 Interfaces Input 1 USB upstream, type B (labeled PC for connecting to a computer) Output 2 USB downstream, type A (labeled USB 1 and USB 2) 2 MSB 8-pin Mini DIN (female) (labeled MSB 1 and MSB 2 for Dosino, stirrer, remote lines, etc.) 2 15-pin high-density D-sub (female) (labeled Detector 1 and Detec- Detector...
  • Page 124 Internet. You can download this information using the article number as follows: Downloading the accessories list 1 Type https://www.metrohm.com/ into your Internet browser. 2 Under Find products, accessories, and applications by, enter the article number (e.g. 2.940.1410).
  • Page 125 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index Crystallization Heating Adsorption cartridges High-pressure pump ... 70 Also see "Column thermostat" Connection ......40 ........... 21 Ambient conditions ....108 Heavy metals Anion suppressor Degasser Contamination of suppressor Regeneration ...... 89 Sample degasser ....44 ........... 89 Aspiration filter (6.2821.090) ..
  • Page 126 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Leak-tightness ......55 Pulsation ........72 Suppression rotor Leakage ........72 Pulsation absorber see Suppressor ..... 89, 91 Leaky piston seals ..... 72 Installation ......27 Suppressor Loop Pump tubing Clean ........91 See also "Sample loop" ..28 Install .........

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