PerkinElmer Raman IdentiCheck Getting Started Manual
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PerkinElmer Raman IdentiCheck Getting Started Manual

PerkinElmer Raman IdentiCheck Getting Started Manual

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MOLECULAR SPECTROSCOPY
Raman IdentiCheck
Getting Started Guide

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Summary of Contents for PerkinElmer Raman IdentiCheck

  • Page 1 MOLECULAR SPECTROSCOPY Raman IdentiCheck Getting Started Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    SOP-4: Collecting a Spectrum with a Triggered Fiber Optic Probe ....56 Calibration and Performance Verification SOPs ...........57 SOP-5: Calibration/Performance Verification ..........57 SOP-6: Wavelength Calibration Verification of the Raman IdentiCheck ..59 SOP-7: Wavelength Calibration of the Raman IdentiCheck ......60 SOP-8: Laser Frequency Calibration of the Raman IdentiCheck .....61...
  • Page 4 Replacing the Triggered Fiber Optic Probe ..........68 Troubleshooting ....................70 Hardware ....................70 Software....................70 Decontamination ..................... 71 WEEE Instructions for PerkinElmer Products ............72 Appendix A: Using your own PC ............... 73 Introduction ....................74 Configuring the PC ................... 75 Installing Spectrum Software ................76...

  • Page 5: Introduction

    Introduction...

  • Page 6: About This Manual

    This chapter outlines the electrical and laser safety issues. It also provides information on the transportation of your Raman IdentiCheck to the site where it is to be used, and setting it up ready for use once you get there. We recommend that all users read this chapter.

  • Page 7: Conventions Used In This Manual

    UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used to show that you have to press two keys at the same time, for example, ALT+F. All eight-digit numbers are PerkinElmer part numbers unless stated otherwise. Notes, Cautions and Warnings Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.
  • Page 8 8 . Raman IdentiCheck Getting Started Guide We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one.
  • Page 9 Introduction . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann.
  • Page 10 10 . Raman IdentiCheck Getting Started Guide...

  • Page 11: Safety Practices

    Safety Practices...

  • Page 12: Overview

    Overview This chapter contains details of safety practices. Everyone who is going to setup and/or use the Raman IdentiCheck must read and follow the precautions before unpacking, installing or using the instrument. Before performing any of the operations outlined in this manual, read all of the safety practices and instructions, and make sure that you understand the procedures explained.

  • Page 13: General Operating Conditions

    WARNING The Raman IdentiCheck spectrometer has been designed and tested in accordance with PerkinElmer specifications and in accordance with the safety requirements of the International Electrotechnical Commission (IEC). The Raman IdentiCheck conforms to...

  • Page 14: Storage

    14 . Raman IdentiCheck Getting Started Guide Whenever it is likely that the Raman IdentiCheck is unsafe, make it inoperative. The Raman IdentiCheck may be unsafe if it: • Shows visible damage • Fails to perform the intended measurement •...

  • Page 15: Electrical Safety

    IEC 61010-1, IEC 60950 or equivalent standards. Power Supplies The Raman IdentiCheck is powered by one external power supply unit. The power supply unit can adjust automatically to a variety of AC power sources as outlined in Table 1.

  • Page 16: Power Cord

    16 . Raman IdentiCheck Getting Started Guide Power Cord The power cord used to connect the instrument power supply unit to the power point (socket) should be appropriate for the country listed as the shipping destination. If the power cord is not appropriate for your country it must be removed from use immediately and replaced.

  • Page 17: Emc Compliance

    EMC Compliance EC directive The Raman IdentiCheck has been designed and tested to meet the requirements of the EC directive 2004/108/EC. The Raman IdentiCheck complies with the EMC standard EN61326 (EMC standard for electrical equipment for measurement, control and laboratory use), and EN55011 (ISM) class A (RF emissions).

  • Page 18: Laser Safety

    18 . Raman IdentiCheck Getting Started Guide Laser Safety Details of the Raman IdentiCheck laser source are summarized are summarized in Table 2. Table 2 Laser Specifications Class Wavelength 785 nm Power output 250 mW (max) The Raman IdentiCheck complies with the requirements of IEC 60825-1:2007 and 21CFR 1040.10 FDA performance standards for laser products, except for deviations...

  • Page 19: Class 3B Safety Precautions

    Safety Practices . 19 Class 3B Safety Precautions You should follow Class 3B laser safety precautions such as the use of door interlocks and the wearing of laser safety goggles. For information on these precautions refer to an appropriately trained laser safety officer within your own facility. Laser safety is very important.

  • Page 20: General Laser Safety Issues

    General Laser Safety Issues Laser Keyswitch There is a captive laser key control switch on the top panel of the Raman IdentiCheck. This key is required to operate the laser. A laser safety officer should control the use of this key.
  • Page 21 7.5 (±10%) 27.3 NOTE: Other focal lengths are available; contact PerkinElmer for details. In particular, the use of an ultra-long working distance lens adaptor (part number L1320071) provides a working distance of 25 mm. Where different focal lengths are used, the NOHD...

  • Page 22: Internal Interlock System

    For more information refer to Interlock Override on page 41. The Raman IdentiCheck has been designed for use outside of a laboratory, with the external interlock overridden. The Raman IdentiCheck should not be left unattended when the laser safety key is in place.

  • Page 23: Warning Labels

    Safety Labels on the Raman IdentiCheck The following labels are displayed on the top panel of the Raman IdentiCheck: LASER RADIATION LASER RADIATION CAUTION –...

  • Page 24: Moving The Instrument

    We recommend using the shipping carton when moving the instrument over long distances. The Raman IdentiCheck weighs approximately 30 kg (including the optional tablet PC and the power supply unit). To avoid injury or damage to the instrument, please use proper lifting techniques.

  • Page 25: Siting The Instrument

    Safety Practices . 25 Siting the Instrument Adverse environmental conditions will affect the quality of the results obtained from the instrument. In particular, major changes in temperature should be avoided. When moving between environments where the temperature difference is more than 10 °C, you should leave the instrument to stabilize before starting to collect data.
  • Page 26 26 . Raman IdentiCheck Getting Started Guide...

  • Page 27: Raman History, Theory And Instrumentation

    Raman History, Theory and Instrumentation...

  • Page 28: The History Of Raman Spectroscopy

    28 . Raman IdentiCheck Getting Started Guide The History of Raman Spectroscopy Inelastic light scattering was first predicted in 1923 by A.G. Smekal in Germany. Research carried out by A. H. Compton discovered inelastic scattering of X-rays, which led the Indian scientist C.

  • Page 29: Raman Theory

    Raman History, Theory and Instrumentation . 29 Raman Theory When photons from a monochromatic light source impinge on a sample, a small fraction are scattered in all directions. Most of the scattered photons are of the same energy as those of the incident beam, and hence the same frequency, giving rise to Rayleigh scattering (Figure 3).
  • Page 30 30 . Raman IdentiCheck Getting Started Guide The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a change in the vibrational and/or rotational energy of the molecule. The frequency shifts from the frequency of the incident beam are displayed on the x-axis of a Raman spectrum, generally in relative wavenumbers (otherwise known as Raman Shift) and the intensity of the bands are displayed in arbitrary units on the y-axis.

  • Page 31: Spectrometers

    Raman History, Theory and Instrumentation . 31 Spectrometers The Raman IdentiCheck includes a Raman spectrometer with laser excitation wavelength at 785 nm. The instrument is composed of a laser, mirrors, fiber optics, filters, lenses, spectrograph and a CCD detector. The laser supplies light through an optical pathway onto the sample through the fiber optic probe.

  • Page 32: Spectrograph Detector Systems

    The Echelle Spectrograph Figure 7 Schematic of the Echelle Spectrograph PerkinElmer’s Echelle detector is shipped in the Raman IdentiCheck. The system contains two components; the Echelle spectrograph and a two ̵ dimensional CCD detector. The system works by dispersing the light in two dimensions by using two gratings (Figure 7). The first grating disperses the light horizontally onto the second grating, where the light is further dispersed vertically onto the CCD detector.
  • Page 33 No light is lost, all the light goes onto the detector, and all the strips are read out simultaneously. The concept of an Echelle detector is not unique to PerkinElmer. Echelle detectors are commonly used in applications where high resolution...
  • Page 34 34 . Raman IdentiCheck Getting Started Guide...

  • Page 35: Introduction To The Raman Identicheck

    Introduction to the Raman IdentiCheck...

  • Page 36: Overview

    36 . Raman IdentiCheck Getting Started Guide Overview A Raman IdentiCheck has the following major components: A Raman spectrometer, built into a transport case, with wheels and a carrying handle • • A power supply unit • A 5 meter triggered fiber optic probe (a 20 meter option is available; as are other types of probe) •...

  • Page 37: Raman Identicheck Specifications

    Power Consumption 50 W (max) If the electricity supply does not conform to these specifications, please consult PerkinElmer prior to installation. If the supply of power is erratic please use an uninterruptible power supply (UPS). Power fluctuations or brown-outs may damage the spectrometer power supply.

  • Page 38: Unpacking The Instrument

    When unpacked, check that you have the following parts and check for any physical damage. If there is any damage, do not continue with the installation. Contact your PerkinElmer Customer Care Representative immediately. Spectrometer Parts...

  • Page 39: Optional Tablet Pc

    Introduction to the Raman IdentiCheck . 39 Optional Tablet PC If you have purchased the optional tablet PC provided by PerkinElmer, the following items will be supplied. Part Number Description L9003694 (Italy) Dell XT-2 Tablet PC L9003646 (US) L9003695 (UK)

  • Page 40: Spectrometer Module

    40 . Raman IdentiCheck Getting Started Guide Spectrometer Module Right-hand Panel The right-hand panel of the Raman IdentiCheck (Figure 10) contains: 3 LED indicators (see table below for details) • • Laser key switch • Air filters for the cooling fans •...

  • Page 41: Left-Hand Panel

    Attaching the External Interlock Override The Raman IdentiCheck has an external interlock connection located on the right-hand side of the top panel (Figure 10). To operate the instrument, the external interlock must be satisfied. This can be achieved in...
  • Page 42 42 . Raman IdentiCheck Getting Started Guide Probe connectors USB port Main power Aux power (covered) Figure 11 Spectrometer module left-hand panel The Aux power connector is covered and should not be used. WARNING...

  • Page 43: Triggered Fiber Optic Probe (Tfop)

    Introduction to the Raman IdentiCheck . 43 Triggered Fiber Optic Probe (TFOP) The Raman IdentiCheck comes fitted with a Triggered Fiber Optic Probe (TFOP), designed to enable the remote measurement of samples. Plastic spacer Trigger Figure 12 Side-view of probe, with plastic spacer fitted, ready for use The probe body contains the filtering optics and electronics, and is interfaced with two fiber optic cables (an excitation fiber and collection fiber).

  • Page 44: Analyzing Samples With The Probe

    44 . Raman IdentiCheck Getting Started Guide Steady blue: Accessory Steady green: Data selected in the software and acquisition in progress probe ready for use Flashing green: Data acquisition complete – release trigger Figure 13 Probe indicators The Probe Path LED lights-up blue when the probe is selected in the Accessory drop-down list on the Setup Instrument Basic tab in Spectrum software, and is ready for use.
  • Page 45 Introduction to the Raman IdentiCheck . 45 Analyzing liquids by immersion The TFOP can only be immersed in liquid if you also use the immersible probe sleeve (part number L1320070). Do not totally submerge the probe; it should be immersed only up to 2.5 cm from the top of the sleeve.

  • Page 46: Power Supply Unit

    46 . Raman IdentiCheck Getting Started Guide Power Supply Unit The power supply unit supplied with the Raman IdentiCheck is shown below. Figure 14 Power supply unit in-situ The power supply unit is designed to operate , strapped within the lid of the IdentiCheck transport case, although it can be removed if required.

  • Page 47: Raman Identicheck Pc

    Raman IdentiCheck PC We recommend that you purchase the tablet PC specifically provided for use with Raman IdentiCheck by PerkinElmer. This conforms to the necessary PC requirements and will be setup, and the necessary PerkinElmer software installed, on-site by a PerkinElmer Service Engineer.

  • Page 48: Commissioning Your Instrument

    ARM position. NOTE: The Raman IdentiCheck is supplied with two keys. One should be placed in the instrument and the other should be stored in a safe place. Replacement keys (part number L1323503) can be obtained from PerkinElmer.
  • Page 49 Introduction to the Raman IdentiCheck . 49 8. Leave the system to equilibrate. When you start the software the instrument will prepare for use. The laser will be turned on. The Laser LED on the instrument and the Probe Path LED on the probe will come on.
  • Page 50 50 . Raman IdentiCheck Getting Started Guide...

  • Page 51: Using The Raman Identicheck

    Using the Raman IdentiCheck...

  • Page 52: Introduction

    SOPs which better suit your specific mode of use. This chapter also acts as a “learn by doing” section allowing you to explore the functionality of the software and spectrometer. NOTE: If you are in any doubt about any aspect of using your Raman IdentiCheck, contact PerkinElmer before proceeding.

  • Page 53: General Sops

    7. Ensure that the interlock is satisfied or the interlock override is fitted. 8. Insert the laser power key into the instrument and turn it to the ARM position. 9. From the Windows Start menu select All Programs; the PerkinElmer Applications group; the Spectrum sub-group and then the Spectrum application.

  • Page 54: Sop-2: Packing Up The Instrument After Use

    54 . Raman IdentiCheck Getting Started Guide 12. Leave the system to equilibrate. When you start the software the instrument will prepare for use. The laser will be turned on. The Laser LED on the instrument and the Probe Path LED on the probe will come on.

  • Page 55: Sop-3: Setting-Up Experimental Parameters

    NOTE: If you intend using PerkinElmer software packages other than Spectrum to carry out data acquisition or analyses, AssureID for example, you should refer to the appropriate user guides and/or on-screen help provided.

  • Page 56: Sop-4: Collecting A Spectrum With A Triggered Fiber Optic Probe

    56 . Raman IdentiCheck Getting Started Guide 7. If required, select any Auto Processing options. These options if selected will be applied to all spectra collected, and cannot be undone. 8. If you select Save after each measurement, your spectra will automatically be saved using the Sample ID.

  • Page 57: Calibration And Performance Verification Sops

    Using the Raman IdentiCheck . 57 Calibration and Performance Verification SOPs Several different automated calibration routines are included in the software: Wavelength (x-axis) calibration uses the position of precisely known lines in a neon • emission spectrum to calibrate the detector.
  • Page 58 58 . Raman IdentiCheck Getting Started Guide Wavelength Calibration Verification Check Laser Key is Laser Frequency turned on and laser Calibration power is set to 100% Fail Fail Check position and Wavelength Completed focus of polystyrene Calibration Peak Intensity sample...

  • Page 59: Sop-6: Wavelength Calibration Verification Of The Raman Identicheck

    Using the Raman IdentiCheck . 59 SOP-6: Wavelength Calibration Verification of the Raman IdentiCheck NOTE: This procedure does not use the trigger. The probe is supported in a clamp stand during the calibration verification. 1. Ensure the instrument is switched on by following SOP-1.

  • Page 60: Sop-7: Wavelength Calibration Of The Raman Identicheck

    60 . Raman IdentiCheck Getting Started Guide SOP-7: Wavelength Calibration of the Raman IdentiCheck Wavelength calibration should only be performed if the instrument has failed a wavelength calibration verification. This procedure does not use the triggered fiber optic probe. It uses an internal neon standard.

  • Page 61: Sop-8: Laser Frequency Calibration Of The Raman Identicheck

    The instrument in now laser frequency calibrated. In the unlikely event that the laser frequency calibration routine should fail, please perform a wavelength calibration and repeat the laser frequency calibration. NOTE: If the calibration fails again, please contact your PerkinElmer Service Representative.

  • Page 62: Sop-9: Intensity Calibration Of The Raman Identicheck

    NOTE: NIST calibration is temperature dependent. If you intend to perform quantitative analyses, you should ensure that your instrument is NIST calibrated at the temperature at which data is to be acquired. Contact your PerkinElmer Raman Business Unit. Otherwise, any data should be used for qualitative analyses only.
  • Page 63 Using the Raman IdentiCheck . 63 In the unlikely event that the intensity calibration routine should fail, please ensure that the laser key switch is set to ARM, the laser power is set to 100%, the NIST sample is correctly positioned...

  • Page 64: Additional Parts And Upgrades

    Ultra-long working distance lens adaptor for Raman fiber optic probes L1320095 Raman probe sampling accessory kit Other Probes and Options The Raman IdentiCheck can also be used in conjunction with a standard fiber optic probe (part number L1320002) and with a RamanMicro 300 microscope. Contact PerkinElmer for details.

  • Page 65: Maintenance And Troubleshooting

    Maintenance and Troubleshooting...

  • Page 66: Maintenance

    66 . Raman IdentiCheck Getting Started Guide Maintenance The system does not require regular maintenance other than occasional checking and cleaning. Cleaning the Spectrometer and Power Supply Units Disconnect the instrument from the power supply unit before cleaning. WARNING You can clean the left- and right-hand panels of the spectrometer using a soft, lint-free cloth, moistened if required with a little water.

  • Page 67: Servicing The Spectrometer

    The TFOP is designed to be maintenance free under normal operating conditions, and should not be opened or adjusted by anyone other than a PerkinElmer Service Engineer. Contact PerkinElmer if you have any questions regarding maintenance or servicing of the TFOP.

  • Page 68: Replacing The Triggered Fiber Optic Probe

    68 . Raman IdentiCheck Getting Started Guide Cleaning the probe body and cable Wipe all external surfaces with a lint-free cloth. If necessary, this cloth may be dampened with a mild detergent solution. Do not use copious amounts of water or allow the water to enter the probe casing.
  • Page 69 Maintenance and Troubleshooting . 69 4. Carefully remove the retained ends of the probe by unscrewing and pulling the cables away from the couplers perpendicularly. Be extremely careful when removing the probe fibers as you can easily damage the instrument and/or the ends of the fibers. 5.

  • Page 70: Troubleshooting

    70 . Raman IdentiCheck Getting Started Guide Troubleshooting This section is a reference guide if you are having any problems with your instrument. Troubleshooting outlines what to do if your instrument or software malfunctions or crashes. Hardware Problem: During the installation procedure the USB devices are not recognized.

  • Page 71: Decontamination

    (8:30 a.m. – 7 p.m. EST) (+1) 203-925-4602 (outside the USA) Customer Care Canada: 800-561-4646 Customer Care EU: 0800 40 858 (Brussels) 0800 90 66 42 (Monza) If you are located outside of these regions, please call your local PerkinElmer sales office for more information.

  • Page 72: Weee Instructions For Perkinelmer Products

    Customer Care department in your region. http://las.perkinelmer.com/OneSource/Environmental-directives.htm Products from other manufacturers may also form a part of your PerkinElmer system. These other manufacturers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive. Please contact these manufacturers directly before discarding any of their products.

  • Page 73: Appendix A: Using Your Own Pc

    Appendix A: Using your own PC...

  • Page 74: Introduction

    PerkinElmer Service Engineer. All that you need to do to commission your Raman IdentiCheck is to unpack and check the instrument, as described on page 38. If you choose to provide your own laptop or tablet PC for use with your Raman IdentiCheck, you must first: •...

  • Page 75: Configuring The Pc

    4. Click OK. You can now start using the PC. 5. It is important that the PC used with Raman IdentiCheck does not switch into Standby mode. Select the Display option on the Control Panel. On the Screen Saver page of the Display Properties dialog, click Power and then set the System standby and Turn off hard disks values to Never for both Plugged in and Running on batteries.

  • Page 76: Installing Spectrum Software

    76 . Raman IdentiCheck Getting Started Guide Installing Spectrum Software You must not connect the instrument USB cable or, if applicable, the triggered fiber optic probe USB cable to the PC before the Spectrum software has been installed. SOP-1: Switching After the software is installed, you should switch on your instrument.

  • Page 77: Configuring Spectrum To Use A Probe

    Appendix A: Using your own PC . 77 Configuring Spectrum to use a Probe 1. Ensure the instrument is switched on and the Spectrum software is running. SOP-1: Switching the IdentiCheck on (page 53) for details. 2. Select Raman Instrument from the Setup menu. The Setup Instrument tabs are displayed.
  • Page 78 78 . Raman IdentiCheck Getting Started Guide...

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