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Summary of Contents for PerkinElmer RamanMicro 200 Series
- Page 1 MOLECULAR SPECTROSCOPY RamanMicro 200 Series Getting Started Guide...
- Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.
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Page 3: Table Of Contents
Preparing Your Facility ................33 Specifications ....................34 Finding a Suitable Location for the Instrument ...........35 Instrument Dimensions ..................36 Installing Your RamanMicro 200 Series ..........37 Unpacking the Instrument ................38 Lifting the Instrument ..................39 Attaching the External Interlock Override............40 Assembly of the Microscope ................41 Fitting the Stage on the Microscope ............41... - Page 4 Maintenance and Service of the Microscope ..........92 Maintenance and Service of the Fiber Optic Probe ........92 Replacing the Stage Controller Fuse ..............93 Troubleshooting ....................94 Hardware ....................94 Software....................94 Decontamination ..................... 96 WEEE Instructions for PerkinElmer Products ............97...
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Page 5: Introduction
Introduction... -
Page 6: About This Manual
This chapter contains a brief overview of the history and theory of Raman spectroscopy. Preparing your Facility This chapter shows you how to position your RamanMicro 200 Series instrument, and what utilities are required, for example electricity and temperature control. -
Page 7: Conventions Used In This Manual
ALT+F. All eight-digit numbers are PerkinElmer part numbers unless stated otherwise. The term RamanMicro 200 Series means the RamanMicro 200 and the RamanMicro 200F Raman Microscopes. - Page 8 8 . RamanMicro 200 Series Getting Started Guide We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one.
- Page 9 Introduction . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann.
- Page 10 10 . RamanMicro 200 Series Getting Started Guide...
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Page 11: Safety Practices
Safety Practices... -
Page 12: Overview
All information provided herein is for reference only. All information is believed to be correct and complete. PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document. PerkinElmer does not accept liability for the interpretation of information resulting, either directly or indirectly, from our spectrometers or associated software interfaces. -
Page 13: General Operating Conditions
50% relative humidity at 40 °C • Mains fluctuations not exceeding ±10% of the nominal voltage. Whenever it is likely that the RamanMicro 200 Series instrument is unsafe, make it inoperative. The RamanMicro 200 Series may be unsafe if it: Shows visible damage •... -
Page 14: Fire Safety
14 . RamanMicro 200 Series Getting Started Guide Fire Safety Ensure that there is at least 15 cm (6”) between any cooling vents and adjacent • equipment or walls. Ensure that the power supply unit (PSU) vents or fan do not become obstructed. -
Page 15: Electrical Safety
100–230 V AC ±10% of the nominal voltage Under no circumstances should you attempt to disassemble the PSU and carry out repairs. If the PSU has a fault, please contact your PerkinElmer Service Representative. The entire unit must be replaced. WARNING Connect the PSU to a three-wire (live, neutral and ground/earth) socket. -
Page 16: Power Cords (Between Psu And Power Outlet Socket)
16 . RamanMicro 200 Series Getting Started Guide Power Cords (Between PSU and Power Outlet Socket) The power cord supplied to connect the PSU to the power point (socket) should be appropriate for the country listed as the shipping destination. -
Page 17: Emc Compliance
EC directive The RamanMicro 200 and RamanMicro 200F have been designed and tested to meet the requirements of the EC directive 2004/108/EC. The RamanMicro 200 Series complies with the EMC standard EN61326 (EMC standard for electrical equipment for measurement, control and laboratory use), and EN55011 (ISM) Class A (RF emissions). -
Page 18: Laser Safety
18 . RamanMicro 200 Series Getting Started Guide Laser Safety Details of the RamanMicro 200 Series laser source are summarized in Table 2. Table 2 Laser Specifications Class Wavelength 785 nm Power output 250 mW (max) The RamanMicro 200 Series complies with the requirements of IEC 60825-1:2007 and 21CFR 1040.10 FDA performance standards for laser products, except for deviations... -
Page 19: Class 3B Safety Precautions
Safety Practices . 19 Class 3B Safety Precautions You should follow Class 3B laser safety precautions such as the use of door interlocks and the wearing of laser safety goggles. For information on these precautions refer to an appropriately trained laser safety officer within your own facility. Laser safety is very important. -
Page 20: General Laser Safety Issues
20 . RamanMicro 200 Series Getting Started Guide General Laser Safety Issues Laser Keyswitch There is a captive laser key control switch on the front panel of the spectrometer. This key is required to operate the laser. A laser safety officer or laboratory manager should control the use of this key. - Page 21 Safety Practices . 21 Do not make any adjustments to the microscope or any part of the system when experiments are in progress. In particular: WARNING Do not remove the objective lens from the microscope • Do not insert or remove samples •...
- Page 22 22 . RamanMicro 200 Series Getting Started Guide Emission indicator (Green LED) Beam attenuator / cover Probe body Fiber optic cable Aperture label Figure 2 Schematic of the fiber optic probe (not to scale) When using the fiber optic probe, the following precautions should be observed: •...
- Page 23 PerkinElmer (part number L1323518). • Do not use the TFOP if it shows any sign of damage. If repair or servicing is needed, call your PerkinElmer Service Representative for advice. • Raman Triggered Fiber Optic Probe...
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Page 24: Internal Interlock System
7.5 (± 10%) 27.3 NOTE: Other focal lengths are available; contact PerkinElmer for details. In particular, the use of an ultra-long working distance lens adaptor (part number L1320071) provides a working distance of 25 mm. Where different focal lengths are used, the NOHD should be calculated accordingly. -
Page 25: Warning Labels
Safety Practices . 25 Warning Labels When this label is attached to an instrument it means refer to the manual to find out the nature of the potential hazard and any actions which have to be taken. Labels on the Exterior of the Spectrometer LASER RADIATION LASER RADIATION AVOID EXPOSURE TO BEAM... -
Page 26: Labels On The Exterior Of The Microscope
26 . RamanMicro 200 Series Getting Started Guide Labels on the Exterior of the Microscope CAUTION – CLASS 3B LASER CAUTION – CLASS 3B LASER RADIATION WHEN OPEN RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM AVOID EXPOSURE TO BEAM LASER APERTURE... -
Page 27: Raman History, Theory And Instrumentation
Raman History, Theory and Instrumentation... -
Page 28: The History Of Raman Spectroscopy
28 . RamanMicro 200 Series Getting Started Guide The History of Raman Spectroscopy Inelastic light scattering was first predicted in 1923 by A. G. Smekal in Germany. Research carried out by A. H. Compton discovered inelastic scattering of X-rays, which led the Indian scientist C. -
Page 29: Raman Theory
Raman History, Theory and Instrumentation . 29 Raman Theory When photons from a monochromatic light source impinge on a sample, a small fraction are scattered in all directions. Some of the scattered photons are of the same energy as those of the incident beam, and hence the same frequency, giving rise to Rayleigh scattering (Figure 6). - Page 30 30 . RamanMicro 200 Series Getting Started Guide The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a change in the vibrational and/or rotational energy of the molecule. The frequency shifts from the frequency of the incident beam are displayed on the x-axis of a Raman spectrum, generally in relative wavenumbers (otherwise known as Raman Shift) and the intensity of the bands are displayed in arbitrary units on the y-axis.
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Page 31: The Ramanmicro 200
Raman History, Theory and Instrumentation . 31 The RamanMicro 200 The RamanMicro 200 is a Raman microscope system with laser excitation wavelength at 785 nm. Alignment camera Raman module Illuminator module Objectives Microscope frame Stage Figure 9 The RamanMicro 200 microscope The microscope (Figure 9) is composed of a frame, illumination module, Raman module and camera module. -
Page 32: The Czerny-Turner Spectrograph
32 . RamanMicro 200 Series Getting Started Guide The Czerny–Turner Spectrograph The RamanMicro 200 is equipped with a Czerny–Turner spectrograph. The Czerny–Turner spectrograph is the most commonly used spectrograph design in Raman spectroscopy. Here, light is focused into the spectrograph, collimated and shone on a grating which disperses that light in one dimension. -
Page 33: Preparing Your Facility
Preparing Your Facility... -
Page 34: Specifications
20 °C – 25 °C Temperature If the electricity supply does not conform to these specifications, please consult PerkinElmer prior to installation. If the supply of power is erratic please use an uninterruptible power supply (UPS). Incorrect shutdown, power fluctuations or brown-outs may damage the... -
Page 35: Finding A Suitable Location For The Instrument
Preparing Your Facility . 35 Finding a Suitable Location for the Instrument The following conditions should be observed: The environment must be suitable for a Class 3B laser system. • • There must be a minimum of 4 power points (1 for the microscope, 1 for the spectrometer and 2 for the PC). -
Page 36: Instrument Dimensions
36 . RamanMicro 200 Series Getting Started Guide Instrument Dimensions The RamanMicro 200 spectrometer module weighs approximately 20 kg. The microscope module weighs approximately 20 kg. Laboratory benching must be strong enough to support the combined weight of the microscope, spectrometer module and any ancillary equipment without warping or wobbling. -
Page 37: Installing Your Ramanmicro 200 Series
Installing Your RamanMicro 200 Series... -
Page 38: Unpacking The Instrument
When unpacked, check that you have the following parts and check for any physical damage. If there is any damage, do not continue with the installation. Contact your PerkinElmer Service Representative immediately. Part Number... -
Page 39: Lifting The Instrument
Installing Your RamanMicro 200 Series . 39 Lifting the Instrument The RamanMicro 200 spectrometer and microscope modules each weigh 20 kg. To avoid injury or damage to the instrument, please use proper lifting techniques. To lift and/or move the instrument safely requires two people. -
Page 40: Attaching The External Interlock Override
40 . RamanMicro 200 Series Getting Started Guide Attaching the External Interlock Override The RamanMicro 200 Series has an external interlock connection located on the rear panel of the spectrometer module. To operate the instrument, the external interlock must be satisfied. -
Page 41: Assembly Of The Microscope
Installing Your RamanMicro 200 Series . 41 Assembly of the Microscope The Olympus BX51 microscope is designed to enable you to analyze microscopic samples. You can purchase a manual stage or a motorized stage. To prevent potential injury to yourself and damage to the instrument,... -
Page 42: Connecting The Motorized Stage
42 . RamanMicro 200 Series Getting Started Guide Connecting the Motorized Stage The motorized stage and joystick (L1865723) are powered by the stage controller box (L1860289). Figure 14 Stage controller and joystick To connect the motorized stage: 1. Connect the cable from the joystick to the socket labeled JOYSTICK on the rear panel of the stage controller box (Figure 15). - Page 43 Installing Your RamanMicro 200 Series . 43 Attaching the Z-axis Focus Motor and Sleeve NOTE: If you have purchased the optional microscope light-proof enclosure, attach the z- axis focus motor and sleeve before fitting the enclosure. See the Raman Microscope Light-Proof Enclosure leaflet (L1321873) for more information.
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Page 44: Attaching The Illumination Source
44 . RamanMicro 200 Series Getting Started Guide Attaching the Illumination Source To attach the illumination source to the illuminator module: 1. Hold the illumination source in position against the rear of the illuminator module. 2. Using the allen key supplied (Figure 17), fasten the two screws so that the source is supported. -
Page 45: Connecting The Raman Module To The Microscope
Installing Your RamanMicro 200 Series . 45 Connecting the Raman Module to the Microscope The Raman module is positioned on top of the illuminator module so that the connecter bracket is aligned above the mounting hole. Ensure that the side of the Raman module is aligned with the illuminator module and then tighten the grub screw. -
Page 46: Connecting The Microscope To The Spectrometer Module
46 . RamanMicro 200 Series Getting Started Guide Connecting the Microscope to the Spectrometer Module The microscope is connected to the spectrometer module by two fiber optic cables. The excitation fiber transmits the laser energy from the spectrometer to the microscope, and the collection cable transmits the collected Raman data back to the detector. - Page 47 Installing Your RamanMicro 200 Series . 47 Make sure that the polarizing ridge on the fiber optic cable is aligned with the slot in the coupler casing before carefully pushing the cable into the fiber bracket. Coupler Polarizing ridge aligned with slot...
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Page 48: Switching On The Instrument
The RamanMicro 200 is supplied with two keys. One should be placed in the instrument and the other should be stored in a safe place. Replacement keys (L1323503) can be obtained from PerkinElmer. A laser safety officer or lab manager can control use of the instrument with these keys. -
Page 49: Installing The Instrument In Spectrum Software
Raman instrument to Spectrum. You should now continue to connect the fiber optic cables for any fiber optic probe or triggered fiber optic probe to the RamanMicro 200 Series instrument, as described in Attaching and Removing the Fiber Optic Probe on page 59. -
Page 50: Alignment Of The Microscope
50 . RamanMicro 200 Series Getting Started Guide Alignment of the Microscope Do not disconnect the microscope from the spectrometer. Only trained PerkinElmer Service Representatives should attach and remove the fiber optic cables that connect the two modules. WARNING The RamanMicro 200 system consists of four primary components: The spectrometer module (containing the laser and the detector) •... -
Page 51: Aligning The Raman Module
(iii) Alignment of the Raman coupler Level (ii) and level (iii) may be carried out without removing any instrument paneling. Level (i) requires removal of instrument paneling and must only be carried out by a PerkinElmer Service Representative, and in a Class 3B environment. - Page 52 52 . RamanMicro 200 Series Getting Started Guide If the system is misaligned, we strongly recommended that you contact a PerkinElmer Service Representative. Changes to the laser path alignment will have a dramatic impact on the performance of your instrument.
- Page 53 Installing Your RamanMicro 200 Series . 53 10. Ensure that you have followed the instructions on the screen, and then click Next. The following dialog is displayed. 11. Adjust the Laser Power until you can see a clear visible spot.
- Page 54 54 . RamanMicro 200 Series Getting Started Guide 16. Click Next. The following dialog is displayed. 17. Use the arrows in the Align Video with Laser dialog to fine-tune the position of the crosshairs to be in the center of the laser spot.
- Page 55 Installing Your RamanMicro 200 Series . 55 10. Ensure that the sample is in focus. 11. Repeat steps 7–10 for each objective until the highest magnification is selected. 12. Switch to the Dark Field (DF) switch position on the microscope, and turn off the illumination.
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Page 56: Moving The Instrument
WARNING Please contact PerkinElmer for advice about removing the fiber optic cables that connect the spectrometer and microscope modules. If you need to ship your instrument you must use the original supplied packing materials to prevent damage to the system. -
Page 57: Using The Ramanmicro 200 Series
Using the RamanMicro 200 Series... -
Page 58: Spectrometer Front Panel
58 . RamanMicro 200 Series Getting Started Guide Spectrometer Front Panel The front panel of the spectrometer module contains 3 LED indicators and 1 key switch (Figure 26). Figure 26 Spectrometer module front panel Description Power If the Power LED is illuminated, this indicates that the instrument is receiving power. -
Page 59: Fiber Optic Probe
DO NOT ATTEMPT TO USE THE PROBE BODY IN SUCH AN ENVIRONMENT WITHOUT THE USE OF AN IMMERSION SLEEVE (L1320003). If required, contact PerkinElmer for further details. Attaching and Removing the Fiber Optic Probe The standard fiber optic probe is constructed such that a single armored cable bifurcates into an excitation cable and a collection cable. - Page 60 60 . RamanMicro 200 Series Getting Started Guide Figure 27 Attaching the FOP 5. Connect the excitation cable of the fiber optic probe to the coupler marked EXC. Make sure that the polarizing ridge on the fiber optic probe cable is aligned with the slot in the coupler casing before carefully pushing the cable into the coupler (Figure 28).
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Page 61: Acquiring Spectra With The Fiber Optic Probe
Using the RamanMicro 200 Series . 61 4. If you have a standard probe, remove the DIN connector in the socket marked LED FIBER 2. If you have a triggered probe, disconnect the USB extender cable from the USB port on your PC. - Page 62 However, immersion in solvents will cause serious damage. Ensure an immersion sleeve is in place before immersing your FOP; see Figure 29. Please contact PerkinElmer if you WARNING have any questions in relation to sampling using the FOP.
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Page 63: General Sops
Using the RamanMicro 200 Series . 63 General SOPs The RamanMicro 200 combined with the Spectrum software is a very flexible instrument. This chapter demonstrates how to perform a number of tasks. The procedures provided may not be the most straightforward or simple for the task you wish to perform, so treat these Standard Operating Procedures (SOPs) as a starting point and with time you may wish to develop your own SOPs which better suit your specific mode of use. - Page 64 7. Switch on the microscope and the microscope illumination. 8. If you have a motorized microscope stage, switch on the stage controller box. 9. From the Start menu select All Programs; the PerkinElmer Applications group; the Spectrum sub-group and then the Spectrum application.
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Page 65: Sop-2: Turning The Instrument Off
Using the RamanMicro 200 Series . 65 SOP-2: Turning the Instrument Off 1. Shut down Spectrum software by selecting Exit from the File menu to return to the Windows operating environment. 2. Switch off the PC and monitor. 3. Switch off the spectrometer by pressing the power switches on the PSUs to OFF: Ο... -
Page 66: Sop-3: Setting The Limits Of The Motorized Stage
66 . RamanMicro 200 Series Getting Started Guide SOP-3: Setting the Limits of the Motorized Stage NOTE: If you move the stage, you may also have to check the alignment of the microscope Aligning the Microscope Objectives objectives. Refer to on page 50. - Page 67 Using the RamanMicro 200 Series . 67 9. Re-secure the fixing screw to fix the height of the stage. 10. Select the Setup Microscope XYZ tab. Figure 32 Setup Microscope XYZ Stage tab showing the Live Video view 11. Select Toolbox and then select Reset Stage from the sub-menu.
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Page 68: Sop-4: Sample Positioning Using A Manual Stage
68 . RamanMicro 200 Series Getting Started Guide 17. Re-secure the hexagonal fixing screw to fix the height of the stage. 18. Select Toolbox and then select Reset Stage from the sub-menu. 19. Follow the prompts on-screen, as before. You will need to repeat this process every time you adjust or install the stage. - Page 69 Using the RamanMicro 200 Series . 69 8. Select Raman Instrument from the Setup menu. The Setup Instrument tabs are displayed. 9. Select the Setup Instrument Basic tab. Figure 34 Setup Instrument Basic tab with the Microscope accessory selected 10. Ensure that Microscope is selected in the Accessory drop-down list.
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Page 70: Sop-5: Sample Positioning Using A Motorized Sample Stage
70 . RamanMicro 200 Series Getting Started Guide 17. Focus on the sample and again move the sample in the directions, if necessary. The crosshairs indicate the spot where analysis takes place. NOTE: If there is an offset between the laser position and the cross hairs, use the Align Aligning Video with Laser tool to adjust this. - Page 71 Using the RamanMicro 200 Series . 71 5. Select the Setup Instrument Basic tab. Figure 36 Setup Instrument Basic tab with the Microscope XYZ Stage accessory selected 6. Ensure that Microscope XYZ Stage is selected in the Accessory drop-down list.
- Page 72 72 . RamanMicro 200 Series Getting Started Guide 10. Select the Setup Microscope XYZ tab, and then select the Live Video view. Figure 38 Setup Microscope XYZ Stage tab showing the Live Video view 11. Use the joystick, or click Stage Position and then use the controls, to move the stage so that the sample is approximately lined up below the objective lens and then focus on the sample.
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Page 73: Sop-6: Setting-Up And Saving Experimental Parameters
Using the RamanMicro 200 Series . 73 17. If the microscope light-proof enclosure is fitted, close the doors. 18. Click The RamanMicro 200 will start collecting data in the Live display. You can use Monitor to help you optimize the Exposure time and Laser power for your sample. - Page 74 74 . RamanMicro 200 Series Getting Started Guide 3. Select Microscope or Microscope XYZ Stage as appropriate from the Accessory drop-down list. Depending on the Accessory you have selected, further options may become available. 4. Ensure that the appropriate Microscope Objective and Sample Holder are selected.
- Page 75 Using the RamanMicro 200 Series . 75 Spectra are always background corrected. If there is not a valid background in memory, a background will be acquired at this point. You will see a progress bar for the background collection on the Live tab. You can enter the number of backgrounds on the Setup Instrument Data Collection.
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Page 76: Fiber Optic Probe Sops
76 . RamanMicro 200 Series Getting Started Guide Fiber Optic Probe SOPs NOTE: The SOPs included on the following pages are only appropriate for a standard fiber optic probe. For details of how to acquire data using a triggered fiber optic probe, see Raman Triggered Fiber Optic Probe leaflet (L1321940). -
Page 77: Sop-8: Sample Positioning When Using A Fiber Optic Probe
Using the RamanMicro 200 Series . 77 5. Ensure that laser safety precautions are being observed, and then click Continue. If you have any doubts about the required safety precautions click Cancel. When the laser is on and the shutter is open the Laser Status icon on the Measurement bar will be updated to Class 3B Laser Status . -
Page 78: Calibration And Performance Verification Sops
78 . RamanMicro 200 Series Getting Started Guide Calibration and Performance Verification SOPs Several different automated calibration tests are included in the software: Wavelength (x-axis) calibration uses the position of precisely known lines in a neon • emission spectrum to calibrate the detector. -
Page 79: Sop-10: Wavelength Calibration Verification
Then repeat the wavelength calibration verification to ensure that instrument has been brought back within specification. If the instrument fails the wavelength calibration verification again, please contact PerkinElmer. NOTE: Stray light could cause the wavelength calibration verification to fail. If a test fails, particularly if you are using the microscope without an enclosure, ensure that no light is entering the Raman system. - Page 80 80 . RamanMicro 200 Series Getting Started Guide 4. Select Raman Instrument from the Setup menu. The Setup Instrument tabs are displayed. 5. On the Setup Instrument Basic tab select Microscope or Microscope XYZ Stage as appropriate from the Accessory drop-down list 6.
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Page 81: Sop-11: Wavelength Calibration
Using the RamanMicro 200 Series . 81 In the unlikely event that the wavelength calibration verification should fail, follow Figure 41. If the wavelength calibration verification fails for the microscope, this does not necessarily mean that the performance of any other accessories attached to your instrument is also affected. -
Page 82: Sop-12: Laser Frequency Calibration
82 . RamanMicro 200 Series Getting Started Guide 9. Ensure that you have followed the instructions on the dialog, and then click Next. The instrument will now perform the wavelength calibration routine. This will take some time. When the calibration is complete, the result is displayed. -
Page 83: Sop-13: Intensity Calibration
In the unlikely event that the laser frequency calibration routine should fail, please perform a wavelength calibration and repeat the laser frequency calibration. NOTE: If the calibration fails again, please contact your PerkinElmer Service Representative. SOP-13: Intensity Calibration The intensity calibration must be performed using the National Institute of Standards and Technology (NIST) Standard Reference Material. - Page 84 NOTE: As intensity calibration is not required on a regular basis, NIST Standards are not included in the basic RamanMicro 200 package. Standard Reference Materials can be purchased from PerkinElmer (L1321831). Your instrument will be intensity calibrated prior to shipping and during installation.
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Page 85: Summary
Using the RamanMicro 200 Series . 85 14. Select The Intensity Calibration dialog is displayed. 15. Ensure that you have followed the instructions on the dialog, and then click Next. The instrument will now perform the intensity calibration routine. This will take some time. -
Page 86: Additional Parts And Upgrades
86 . RamanMicro 200 Series Getting Started Guide Additional Parts and Upgrades Microscope Objective Lenses Part Number Description L1323336 100x objective lens L1323347 Long working distance objective lenses upgrade kit Contains 5x and 20x long working distance objective lenses L1323343... -
Page 87: Sers Consumables
Using the RamanMicro 200 Series . 87 SERS Consumables Part Number Description L1329300 SERS gold colloid – 50 nm particles (20 ml) L1329307 SERS gold colloid – 100 nm particles (20 ml) L1329308 SERS gold colloid – 200 nm particles (20 ml) L1329301 Aggregating agent –... -
Page 88: Fiber Optic Probes
88 . RamanMicro 200 Series Getting Started Guide Fiber Optic Probes Part Number Description Raman fiber optic probe, 7.5 mm working distance, maximum working L1320002 temperature 80 °C, 5 M cabling L1320003 Liquid immersion sleeve for Raman fiber optic probe Raman probe –... -
Page 89: Maintenance And Troubleshooting
Maintenance and Troubleshooting... -
Page 90: Maintenance
90 . RamanMicro 200 Series Getting Started Guide Maintenance The system does not require regular maintenance other than occasional checking and cleaning. Cleaning the Spectrometer and Power Supply Units Switch off the mains voltage and disconnect the mains cord before cleaning. -
Page 91: Cleaning The Air Filters
Maintenance and Troubleshooting . 91 Cleaning the Air Filters The air filters are located inside the top panel of the spectrometer module. You should clean the filters regularly. To clean the filters: 1. Switch off the instrument power supply unit. 2. -
Page 92: Servicing The Spectrometer
Maintenance and Service of the Fiber Optic Probe The FOP is designed to be maintenance free and should not be opened or adjusted by anyone other than a PerkinElmer Service Engineer. Contact PerkinElmer if you have any questions regarding maintenance or servicing of the FOP. -
Page 93: Replacing The Stage Controller Fuse
Maintenance and Troubleshooting . 93 Replacing the Stage Controller Fuse NOTE: This section is only applicable if you have a motorized stage. Electrical hazard To prevent potential injury to yourself and damage to the instrument, switch OFF all components in the system and disconnect them from the line power supply before you alter, or make any new, electrical WARNING connections. -
Page 94: Troubleshooting
94 . RamanMicro 200 Series Getting Started Guide Troubleshooting This section is a reference guide if you are having any problems with your instrument. Troubleshooting outlines what to do if your instrument or software malfunctions or crashes. Hardware Problem: During the installation procedure the USB devices are not recognized. - Page 95 Reset Stage. The motorized accessory will now reset itself. Solution 4 If the above solutions do not solve your problem, you should contact your PerkinElmer Service Representative. Problem: The message “Video Image not available” is displayed on the Live Video image on the Setup Microscope XYZ Stage tab.
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Page 96: Decontamination
(8:30 a.m. – 7 p.m. EST ) (+1) 203-925-4602 (outside the USA) Customer Care Canada: 800-561-4646 Customer Care EU: 0800 40 858 (Brussels) 0800 90 66 42 (Monza) If you are located outside of these regions, please call your local PerkinElmer sales office for more information. -
Page 97: Weee Instructions For Perkinelmer Products
Customer Care department in your region. http://las.perkinelmer.com/OneSource/Environmental-directives.htm Products from other manufacturers may also form a part of your PerkinElmer system. These other manufacturers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive. Please contact these manufacturers directly before discarding any of their products. - Page 98 98 . RamanMicro 200 Series Getting Started Guide...