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Install
Fraction collector F9-R
Instructions
Scope
Instructions on how to install one, or for ÄKTA™ pure two, optional Fraction collector
F9-R (29011362) modules.
Description
Introduction
Fraction collector F9-R collects fractions from ÄKTA avant and ÄKTA pure purification
runs.
Fraction collector F9-R can be used for:
•
Time or volume based fractionation
•
Peak fractionation
•
Combined time or volume based fractionation and peak fractionation
It is possible to install up to two Fraction collector F9-R modules when using ÄKTA pure.
ÄKTA avant has a built-in fraction collector, and it is possible to install one Fraction
collector F9-R as an extra module.
Note:
cytiva.com
If two fraction collectors are to be used, the second one must be configured as
Fraction collector F9-R 2nd, independent of the first fraction collector type.
29021462 AD
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Related Manuals for cytiva AKTA F9-R
Summary of Contents for cytiva AKTA F9-R
- Page 1 ÄKTA avant has a built-in fraction collector, and it is possible to install one Fraction collector F9-R as an extra module. Note: If two fraction collectors are to be used, the second one must be configured as Fraction collector F9-R 2nd, independent of the first fraction collector type. cytiva.com 29021462 AD...
- Page 2 Location ÄKTA pure The illustration below shows Fraction collector F9-R together with ÄKTA pure. It is recommended to place Fraction collector F9-R on the left side of the instrument. Fraction collector F9-R can also be placed on a shelf located beneath the system. ÄKTA avant In ÄKTA avant it is recommended to place Fraction collector F9-R on the right side of the instrument.
- Page 3 Note: Consider the tubing going to Fraction collector F9-R if ÄKTA avant is turned on its swivel foot. ÄKTA avant and ÄKTA pure Instructions 29021462 AD...
- Page 4 Illustration of Fraction collector F9-R Part Function Lock knob Stationary part of delivery arm Delivery arm Tubing connector Tube sensor Collection tubes Tube rack Base unit ÄKTA avant and ÄKTA pure Instructions 29021462 AD...
- Page 5 Unpacking Follow the instruction below to unpack the Fraction collector and to remove the transport fixations. Step Action Open the transportation box. Lift off the piece of foam and check the content according to the supplied package list. ÄKTA avant and ÄKTA pure Instructions 29021462 AD...
- Page 6 Step Action Lift out Fraction collector F9-R by the Base unit and place it on the bench. NOTICE Never lift Fraction collector F9-R by the Delivery arm. This may damage the Fraction collector. Remove the plastic bag. ÄKTA avant and ÄKTA pure Instructions 29021462 AD...
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Page 7: Installation
Step Action Lift the Delivery arm and gently move the Delivery arm to the outer stop. Remove the piece of foam from under the Tube rack. Installation For information on how to assemble Fraction collector F9-R, please refer to the respective instrument User manual and Fraction collector F9-C and F9-R Operating instructions. - Page 8 Illustration of Fraction collector F9-R connector panel Part Function Node ID switch UniNet-9 communication and power supply Connect the Fraction collector Connect the UniNet cable between any of the UniNet-9 ports on the back of the instrument and on the back of the Fraction collector F9-R or Fraction collector F9-R, 2nd. Make sure that all unused UniNet-9 ports on the instrument are plugged.
- Page 9 Step Action Set Node ID to 0 for Fraction collector F9-R and to 1 for Fraction collector F9- R, 2nd. Connect Fraction collector F9-R and/or Fraction collector F9-R, 2nd to the instrument. ÄKTA avant and ÄKTA pure Instructions 29021462 AD...
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Page 10: Software Configuration
Software configuration When a Fraction collector has been installed, the System Properties for the system must be updated in UNICORN™. The system will restart automatically when the configuration has been changed and the system can be reconnected. Follow the instructions below to update the system in UNICORN. Step Action •... - Page 11 Step Action Select Fraction collectors from the Component types list. Result: All available fraction collectors are shown in the Component selection list. • Click the checkbox to select the added fraction collector. Note: Instrument modules are referred to as Components in UNICORN. Click OK to apply the changes.
- Page 12 Delay volumes for ÄKTA avant and ÄKTA pure modules and standard tubing configurations are found in the respective User Manual. Follow the instructions below to check/set the delay volumes: Step Action On the System menu in the System Control module, click Connect to Systems or click the Connect to Systems icon.
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Page 13: Connect Tubing
Connect tubing Follow the instructions in the table below to connect the tubing from ÄKTA avant and ÄKTA pure to Fraction collector F9-R, or to both Fraction collector F9-R and Fraction collector F9-R, 2nd. Step Action Lift out the Tubing holder (4) from the Delivery arm (1). Part Function Delivery arm... - Page 14 Step Action Place the Tubing holder with the tubing over the Tube adjustment cavity (2) of the Delivery arm. Push the tubing down against the bottom of the Tube adjustment cavity, and then fingertighten the Tubing holder nut. This ensures the correct length of the exposed tubing end (6).
- Page 15 Standard tubing dimensions The table below shows recommended standard tubing dimensions to connect Fraction collector F9-R. System Tubing Tubing diameter (mm) length (mm) ÄKTA pure 25 0.50 ÄKTA pure 150 0.75 ÄKTA avant 25 0.50 ÄKTA avant 150 1.00 Note: Precut standard tubing for ÄKTA pure are delivered with the fraction collector.
- Page 16 Standard Software End-User License Agreement is available on request. All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.
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