ThermoFisher Scientific invitrogen Neon NxT NEON18S User Manual

Electroporation system, for electroporation of mammalian cells, including primary and stem cells, with high transfection efficiency

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Neon

NxT Electroporation System

™

USER GUIDE

For electroporation of mammalian cells, including primary and

stem cells, with high transfection efficiency

Catalog Numbers NEON18S, NEON1S, NEON18SK, NEON1SK

Publication Number MAN0026677

Revision B.0

For Research Use Only. Not for use in diagnostic procedures.

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Summary of Contents for ThermoFisher Scientific invitrogen Neon NxT NEON18S

Page 1 Neon NxT Electroporation System ™ USER GUIDE For electroporation of mammalian cells, including primary and stem cells, with high transfection efficiency Catalog Numbers NEON18S, NEON1S, NEON18SK, NEON1SK Publication Number MAN0026677 Revision B.0 For Research Use Only. Not for use in diagnostic procedures.
Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. Revision history: B.0 MAN0026677 (English) Revision Date Description 22 October 2024 Addition of new Resuspension Genome Editing Buffer, and 8-channel pipette and pipette station.
Page 3 Contents ■ CHAPTER 1 Product information ..........6 Product description .
Page 4 Contents ■ CHAPTER 2 Methods ............. 37 Electroporation protocol options .
Page 5 Contents ■ APPENDIX B Optimization ............73 Optimization protocol .
Page 6 Product information Product description The Neon NxT Electroporation System is a benchtop electroporation device that employs an ™ electroporation technology which uses a pipette tip as an electroporation chamber to efficiently transfect mammalian cells including primary and immortalized hematopoietic cells, stem cells, and primary cells.
Page 7 Chapter 1 Product information Product description Features Important features of the Neon NxT Electroporation System are listed below: ™ • Small footprint—the benchtop design fits inside a tissue culture hood, reducing contamination risk. • Preserves samples—minimize sample transfer loss with the complete elimination of an electroporation cuvette and associated pipetting steps.
Page 8 Chapter 1 Product information Product contents Product contents Neon NxT System contents ™ The contents of the Neon NxT Electroporation System are listed in the following table. The Neon ™ ™ Electroporation System is shipped at room temperature. page 11 for descriptions of the Neon NxT Electroporation System, and page 37 to set up the...
Page 9 Chapter 1 Product information Product contents Neon NxT Kit contents ™ Neon NxT Kits are used with the Neon NxT Electroporation System for efficient transfection of ™ ™ mammalian cells and are available as standalone products (see “Accessory products” page 85). The kits consist of two components which are not sold individually (a Tips/Tubes Kit, and a Buffer Kit), and are available in two formats (for electroporation of 10 µL samples, and 100 µL samples).
Page 10 Chapter 1 Product information Product contents Table 2 Consumables for use with the Neon NxT 8-Channel Pipette ™ Neon NxT Kit, 10 µL Neon NxT Kit, 100 µL ™ ™ Cat. No. Cat. No. Cat. No. Cat. No. Item N1096-8 N10384-8 N10096-8 N100384-8 (192 reactions) (768 reactions) (192 reactions) (768 reactions) N1096K8...
Page 11 Chapter 1 Product information System components System components Neon NxT device ™ The electrical parameters of the Neon NxT device are factory set and do not require calibration. It ™ is used with Neon NxT Pipette Stations and Neon NxT Kits to efficiently transfect mammalian cells ™...
Page 12 Chapter 1 Product information System components LED status indicators LED color Status Blue Instrument is idle. Green Instrument is in operation. Blinking green Instrument high voltage subsystem in operation. Blinking amber Warning/error About the covered ports Do not access the covered ports at the rear of the device during electroporation. The ports should remain covered during the protocol, and should only be accessed when the device is in idle mode.
Page 13 Chapter 1 Product information System components Neon NxT 1-Channel Pipette Station ™ The Neon NxT 1-Channel Pipette Station holds a Neon NxT 1-Channel Pipette during electroporation ™ ™ procedures. The pipette (with attached tip) is held in the Neon NxT 1-Channel Tubes which has an ™...
Page 14 Chapter 1 Product information System components Using the Neon NxT 1-Channel Pipette ™ Attach a ClipTip by pushing down on a tip until a click is Press the plunger to the second stop to engage the piston ™ heard. (whereupon a click is heard). Press the plunger to the first stop, then slowly release to Press the plunger...
Page 15 Chapter 1 Product information System components Press the tip ejector to disengage the ClipTip , then press ™ the plunger to the second stop to release and eject the piston. Neon NxT 8-Channel Pipette Station ™ The Neon NxT 8-Channel Pipette Station holds a Neon NxT 8-Channel Pipette during electroporation ™...
Page 16 Chapter 1 Product information System components Neon NxT 8-Channel Pipette ™ The Neon NxT 8-Channel Pipette utilizes a positive ™ displacement pipette mechanism for pipetting mixtures containing cells and payload (such as nucleic acid). The Neon NxT 8-Channel Pipette is a fixed volume pipette ™...
Page 17 Chapter 1 Product information System components Press the Dispense plunger until it is flush with the top of the pipette to: • Engage the pipette with the pistons in the tips. • Dispense samples. Press the Aspirate plunger • Retract pistons. • Aspirate samples. Neon NxT Electroporation System User Guide ™...
Page 18 Chapter 1 Product information System components Press the Eject button • Eject the tips. • Release the piston engagement button (when the Dispense plunger is all the way down and flush with the top of the pipette). Neon NxT Kit ™ Neon NxT Kits contain Neon NxT Tips, Neon NxT Tubes, and buffers for electroporation.
Page 19 Chapter 1 Product information System components Neon NxT 1-Channel Tubes ™ Neon NxT 1-Channel Tubes hold electrolytic buffer during ™ electroporation and are inserted into the Neon NxT 1- ™ Channel Pipette Station. The Neon NxT 1-Channel Pipette ™ with a Neon NxT Tip is then inserted into the Neon ™...
Page 20 Chapter 1 Product information Upon receiving the device Upon receiving the device Examine the unit carefully for any damage incurred during transit. Any damage claims must be filed with the carrier. If the instrument is damaged during shipment, contact Technical Support for assistance.
Page 21 Chapter 1 Product information Getting started 4. Connect the high voltage and low voltage interface connectors on the cable to the high voltage and low voltage interface ports at the rear of the Neon NxT Pipette Station. ™ Note: To avoid damaging the cable connector, ensure the notch on the low voltage connector is aligned with the corresponding slot on the low voltage port.
Page 22 Chapter 1 Product information Getting started 5. Connect the high voltage and low voltage interface connectors on the Neon NxT Pipette Station ™ to the high voltage and low voltage interface ports at the rear of the Neon NxT device. ™ Note: To avoid damaging the cable connector, ensure the notch on the low voltage connector is aligned with the corresponding slot on the low voltage port.
Page 23 Chapter 1 Product information Getting started 7. Attach the power cord to the AC inlet at the rear of the Neon NxT device, then plug the cord into ™ an electrical outlet. Use only properly grounded AC outlets and power cords. The instrument operates at voltages of 100–240 VAC and the frequency range of 50/60 Hz. Ensure that the local supply voltage in the laboratory conforms to that specified on the type label on the back of the instrument 8.
Page 24 Chapter 1 Product information Getting started User interface overview Symbol Function Main dial • Set up run • Select a protocol or plate map • Create a new protocol (see “Create or edit protocols” on page 38 for more details) • Create a new plate map (see “Create or edit plate maps” on page 40 for more details) Displays the instrument status when a protocol is run (see “Touchscreen status indicators”...
Page 25 Chapter 1 Product information Getting started Touchscreen status indicators Table 4 Status indicators Button Function Indicates whether a USB device is inserted into the instrument. Indicates whether the Wi-Fi is on or off. Indicates whether the instrument is connected to wired network. Indicates whether the instrument is connected to the Thermo Fisher Connect ™...
Page 26 Chapter 1 Product information Getting started Enter numbers When you press a field that requires a numerical input, the numeric editor, as seen in the following figure, opens. Enter a number Close and save Delete/backspace Close without saving Neon NxT Electroporation System User Guide ™...
Page 27 Chapter 1 Product information Getting started Connect the instrument to the Internet The instrument can connect to the Internet by either wired or wireless methods. To connect by wired method through the instrument Ethernet port using a cable, see “Set up a wired connection”...
Page 28 Chapter 1 Product information Getting started 3. In the Instrument Settings screen, select Network configuration. 4. In the Network Connection screen, select a field in the Wired panel. Wireless panel Wired panel 5. Select a method to enter an IP address. a. Select DHCP to obtain an IP address automatically. A check mark appears when DHCP is selected.
Page 29 Chapter 1 Product information Getting started b. Select Static IP to enter an IP address manually, then enter the appropriate IP addresses for the instrument, the Subnet Mask, and, optionally, the Default Gateway, the Primary DNS Server, and the Secondary DNS Server using the numeric editor. Addresses are in the form of X.X.X.X, where each X is a 3- digit number, from 001 to 255.
Page 30 Chapter 1 Product information Getting started Set up a wireless connection Connect the High-Power USB Wi-Fi Module (Cat. No. A26774 to the USB wireless adapter port (see “Front and rear view” on page 11 for port location). 1. See “Set up a wired connection” on page 27 Steps 1 through 3 to find the Network configuration screen.
Page 31 Chapter 1 Product information Getting started • Open • WPA2 Personal • WEP • WPA Enterprise • WPA Personal • WPA2 Enterprise Note: The above options are available only if Join Other Network was selected in Step 3. You cannot change the security type if you selected an existing network. 6.
Page 32 Chapter 1 Product information Getting started Change a user PIN 1. Select Edit. 2. Enter the old PIN. 3. Enter a new four digit PIN. 4. Re-enter the new PIN, then select Done. Delete a user PIN If a user PIN is forgotten, an administrator can delete the existing PIN to allow a new one to be created. This function resets a PIN, so the user with a deleted PIN is prompted to create a new PIN the next time they sign in) 1.
Page 33 Chapter 1 Product information About the Thermo Fisher Connect Platform ™ About the Thermo Fisher Connect Platform ™ The Thermo Fisher Connect Platform enables access to the Neon NxT device through ™ ™ InstrumentConnect by way of a web browser or mobile device. This cloud-based tool allows the user to perform the following functions when the instrument has Internet connectivity.
Page 34 Chapter 1 Product information About the Thermo Fisher Connect Platform ™ Set up a new administrator 1. To set up a new adminstrator, log in to current administrator Connect Platform account. 2. Select Instruments. 3. Select the Neon NxT device for the current administrator. ™...
Page 35 Chapter 1 Product information About the Thermo Fisher Connect Platform ™ Add an instrument to your Connect account (PC) 1. Log in to your Connect account using a web browser. 2. Select (InstrumentConnect) from the left navigation strip. 3. Select (Add an Instrument) from the top navigation strip. 4.
Page 36 Chapter 1 Product information About the Thermo Fisher Connect Platform ™ 6. Transfer the license to a USB drive. Note: The license should be saved in the root directory of the USB drive, and not in a folder or subdirectory. 7. Select Settings4Maintenance & Servies4License upgrade. 8.
Page 37 Methods Electroporation protocol options If a protocol with the necessary electroporation parameters for your cell type already exists, the protocol can be chosen from the Protocol Library by selecting Library4Protocol Library. • The Protocol Library contains a list of all available protocols stored on the instrument. Swipe up or down in the list pane to scroll through the protocols.
Page 38 Chapter 2 Methods Create or edit protocols The individual parameters (voltage, pulse width, pulse number) have their own independent upper limits. However, some combinations of these variables can exceed the energy limit of the system even if still within the individual limit. For example, a protocol of 2300 V/30 ms/3-pulses has all variables within acceptable limits, but cannot be run as it exceeds the energy limit of the system.
Page 39 Chapter 2 Methods Create or edit protocols Edit a protocol 1. In the Home screen, select Set up run4Quick run, or select Library. 2. Select a text field or open a dropdown menu to set the electroporation parameters for the protocol. 3. Select Save protocol (see “Save a Protocol” page 39), Electroporate to run the protocol, or Cancel.
Page 40 Chapter 2 Methods Create or edit plate maps Create or edit plate maps Plate maps are used to plan out how cells are plated after electroportation. Fill the wells where cells will be plated with the appropriate cell culture media. Create a plate map A plate map is created from the plate view.
Page 41 Chapter 2 Methods Create or edit plate maps d. Select Done to assign a protocol to the selected wells. 6. Select Save plate and proceed to electroporation, or perform other actions. a. Select Save plate (see “Save a plate map” page 42). b. Select Next to proceed to electroporation. c.
Page 42 Chapter 2 Methods Manage protocols 4. Select Save protocol (see “Save a Protocol” page 39), Clear,Cancel, or Done. 5. Select Save plate (see “Save a Protocol” page 39), Next, Cancel, or Actions. Save a plate map 1. Once edits to a protocol are complete, select Save to save the plate map. 2.
Page 43 Chapter 2 Methods Manage protocols Import a protocol 1. Select Library4Import. 2. Select the location from which the files will be imported (Thermo Fisher Connect Platform, USB ™ drive, network drive) Note: To make a network drive connection, see “Set up or change a network drive connection” page 45.
Page 44 Chapter 2 Methods Manage protocols 3. Select the protocols to import, then select Import. Export a protocol 1. Select Library4Protocol Library, then select the protocols to be exported. Neon NxT Electroporation System User Guide ™...
Page 45 Chapter 2 Methods Manage protocols 2. Select Export, then select the destination for the files to be exported (Thermo Fisher Connect ™ Platform, USB drive, network drive). Note: To make a network drive connection, see “Set up or change a network drive connection” page 45.
Page 46 Chapter 2 Methods General guidelines 2. Enter the network drive details (drive location, domain name, user name, password) in the Network Drive screen, then select Connect. General guidelines Guidelines for using kits To use the Neon NxT device for electroporation of mammalian cells, you need to purchase the Neon ™...
Page 47 Chapter 2 Methods General guidelines · Visit thermofisher.com/transfectionprotocolsandcitations for a library of electroporation protocols for commonly used cell types. These protocols are also stored on the instrument. Ensure the instrument has the latest version of the software for access to the most current protocols. Guidelines for selecting buffers There are different cell resuspension buffers available for use with the Neon NxT Electroporation...
Page 48 Chapter 2 Methods General guidelines DNA quality and amount The quality and concentration of DNA used for electroporation plays an important role for the transfection efficiency. We strongly recommend using high quality plasmid purification kits such as PureLink HiPure Plasmid DNA Purification Kits (see page 85) to prepare DNA. ™...
Page 49 Chapter 2 Methods Using the Neon NxT Electroporation System ™ siRNA control The success of electroporation is influenced by the type and quality of siRNA used. Therefore, using both a positive and negative control to evaluate the effectiveness of transfecting with siRNA is recommend.
Page 50 Chapter 2 Methods Using the Neon NxT Electroporation System ™ IMPORTANT! · To obtain the highest transfection efficiency, optimize transfection conditions by varying electrical parameters as described in Appendix B, “Optimization” using preprogrammed optimization protocols. · Since the cell culture conditions vary from user to user, be sure to use low passage number, actively dividing cells (for dividing cells) Calculate electroporation volumes for cell suspension Select and enter the appropriate parameters into the calculator to determine the appropriate volumes of...
Page 51 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Amount of reagents For each electroporation sample, the typical amount of payload, cell number, and volume of plating medium per well are listed in the following table. Volume of siRNA R, T, or Genome Format Cell Type DNA (µg)
Page 52 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Prepare adherent cells 1. Cultivate the required number of cells (70–90% confluent on the day of transfection) by seeding a flask containing fresh growth medium 1–2 days prior to electroporation. For most optimized protocols, seed with: •...
Page 53 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Prepare suspension cells 1. Cultivate the required number of cells (cell density ∼1–3 × 10 cells/T-25 flask) by seeding a flask containing fresh growth medium 1–2 days prior to electroporation. For most optimized protocols, seed with: •...
Page 54 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Set up the pipette station 1. Ensure the Neon NxT Pipette Station is connected to the Neon NxT device (see 20). ™ ™ 2. (If necessary) Attach the Tube Chamber for Neon NxT Tubes to the Neon NxT Pipette Station.
Page 55 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Load the pipette For details on how to handle Neon NxT pipettes, see “Using the Neon NxT 1-Channel Pipette” ™ ™ page 14 or “Using the Neon NxT 8-Channel Pipette” page 16. ™ 1. Open the box containing the type of Neon NxT Tip to be used for electroporation.
Page 56 Chapter 2 Methods Using the Neon NxT Electroporation System ™ 7. Dock the Neon NxT Pipette with the sample vertically into the Neon NxT Tube placed in the ™ ™ Neon NxT Pipette Station until it clicks into place. Ensure that the pipette is securely docked in ™...
Page 57 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Run electroporation protocol 1. Ensure that the recommended amount of payload, cell number, and volume of plating medium is used for each electroporation sample. 2. Select the protocol or plate map that you want to use to perform electroporation. a.
Page 58 Chapter 2 Methods Using the Neon NxT Electroporation System ™ 5. After delivering the electric pulse, Complete is displayed on the main dial to indicate that electroporation is complete. Protocol completed normally Protocol failed (select the circle to view the error message) 6.
Page 59 Chapter 2 Methods Using the Neon NxT Electroporation System ™ Post-electroporation process After electroporation, allow the electroporated cells to recover by incubating at 37°C for 24 hours. For 100 μL reactions, an intermediate 96-well plate or PCR strips can be used before transferring cells to the final culture vessel.
Page 60 Chapter 2 Methods Maintenance Maintenance Cleaning and maintenance CAUTION! Cleaning and decontamination. Use only the cleaning and decontamination methods specified in the user documentation. It is the responsibility of the operator (or other responsible person) to ensure the following requirements are met: ·...
Page 61 Chapter 2 Methods Maintenance Replace the fuses Required materials • Two IEC/UL listed fuses, rated 10 A, type T (time-lag), 250 VAC, 5 × 20 mm • Small flat-tip screwdriver Replace fuses DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock, which could cause physical injury or death, can result from working on an instrument when the high voltage power supply is operating.
Page 62 Chapter 2 Methods Maintenance Determine firmware version on instrument When a new firmware version is released, you may be required to load the new firmware on the instrument. You will need a USB memory device and, if your instrument requires login, the login details to upgrade the firmware.
Page 63 Chapter 2 Methods Maintenance Upgrade the instrument firmware (USB drive) IMPORTANT! You cannot upgrade the firmware while a run is in progress. 1. Insert the USB memory device (FAT32 format file system) with the new firmware in the USB port of your instrument. Note: For instruments with the USB shortcuts feature enabled, you will be directed to the USB shortcuts screen.
Page 64 Chapter 2 Methods Maintenance Self Verification Test Use the Self Verification Test feature to check the instrument hardware. The check includes testing the high voltage system and other components. Carry out the Self Verification Test periodically or whenever there is an intermittent instrument error. Contact your service representative in case of block failure.
Page 65 Chapter 2 Methods Maintenance Restore factory settings (Administrator profile only) Select Restore factory settings to remove all the data and customized settings and revert to factory settings. All data and settings will be erased once factory settings are restored. At the end of the restoration process, the message, "Your instrument has been restored."...
Page 66 Troubleshooting Observation Possible cause Recommended action No power (the AC power cord is not Check AC power cord connections at both ends. Use the display remains connected. correct cords. blank when the power is turned on) Cannot detect The pipette is not fully Ensure the Neon NxT Tube is inserted properly in the ™...
Page 67 Appendix A Troubleshooting Maintenance Observation Possible cause Recommended action Connection failure Wrong buffers used. Use electrolytic buffer (Buffer E for 10 µL tip and Buffer (continued) E2 for 100 µL tip) in the Neon NxT Tube and sample ™ in Resuspension Buffer in the Neon NxT Tip.
Page 68 Appendix A Troubleshooting Instrument error codes Observation Possible cause Recommended action Irreproducible Multiple use of Neon Do not use the same Neon NxT Tip more than twice. ™ ™ transfection Tip and Neon NxT Tube. Repeated application of electric pulses reduces the tip ™...
Page 69 Appendix A Troubleshooting Instrument error codes (continued) Error message Possible cause Action Mange protocol or Mange plate Selected protocols/plates Delete default protocols Check selected protocols/plates and try cannot be deleted again. Quick run setup Connect pipette station and Pipette station or transparent Connect the pipette station and ensure the ensure transparent safety guard is not connected or...
Page 70 Appendix A Troubleshooting Instrument error codes (continued) Error message Possible cause Action High voltage safety fault Discharging failed, unable to Do not power off instrument or discharge within time before touch/remove the pipette until voltage is the next run down to safe level/allowed to continue. Profile errors Invalid PIN.
Page 71 Appendix A Troubleshooting Instrument error codes (continued) Error message Possible cause Action Unable to recover from Fatal error Reboot instrument, run a self-test. If upgrade failure. – 0x8867 the error is persistent contact service representative. Unknown initialization error. – 0x8855 Power supply voltage exceeded upper or lower limits.
Page 72 Appendix A Troubleshooting Instrument error codes (continued) Error message Possible cause Action Discharge resistor is faulty – Fatal error Reboot instrument, run a self-test. If 0x8303 the error is persistent contact service representative. High Voltage CAP charger is faulty – 0x8301 Neon NxT Electroporation System User Guide ™...
Page 73 Optimization Optimization protocol Electroporation is a process involves applying electrical energy to cells to achieve temporary pores that allow for the transfer of genetic material across the cell membrane. The process is dependent on the combination of electric parameters such as voltage, pulse width, pulse interval, and pulse number. •...
Page 74 Appendix B Optimization Optimization protocol Optimization of higher voltage programs A notable advantage of the Neon NxT 8-channel system is its flexibility in applying different programs ™ to different wells. This feature simplifies the optimization of multiple programs for specific applications with minimal effort. Users have the freedom to choose any combination of these parameters. However, it is important to ensure that the total electrical energy for the pulse discharge remains within a certain limit.
Page 75 Appendix B Optimization Optimization protocol Table 5 Higher voltage protocols (continued) Protocol No. Voltage Pulse width Pulse number 2000 2100 2200 2300 2400 2500 2000 2100 2200 2300 2400 2500 1800 1900 2000 2100 Neon NxT Electroporation System User Guide ™...
Page 76 Recommended instrument settings About Instrument Select Settings4About Instrument to find out more information about the instrument (e.g., firmware version and instrument statistics). • Select EULA to view the End User License Agreement, or download it to a USB drive. • Select Check updates to find out if updates are available for the instrument . Recommended instrument settings Select (Settings) and access the Settings screen to configure the instrument.
Page 77 Appendix C Recommended instrument settings Recommended instrument settings Instrument settings (Administrator only) Select Instrument settings to set the following instrument parameters. • Instrument name Select the Instrument name field to activate the text editor. Enter up to 25 alphanumeric characters to identify the instrument. Note: The instrument name cannot have spaces.
Page 78 Appendix C Recommended instrument settings Recommended instrument settings • Sleep mode Use the Off and On toggle to disable or enable sleep mode. In the 'On' mode, select the Edit Time field to activate the numeric editor to set the time after which the instrument will go from idle mode to standby mode.
Page 79 Appendix C Recommended instrument settings Recommended instrument settings • Brightness Use the slider to adjust the brightness of the touch screen. • Network configuration Use the toggle to select the type of network connection to connect the instrument to the Internet . For details on using the Wireless and Ethernet options, see “Connect the instrument to the Internet”...
Page 80 Appendix C Recommended instrument settings Maintenance & services Maintenance & services Select Maintenance & Services to set the following instrument parameters. • (Administrator only) Select Software Update to update the System firmware. See “Upgrade the system firmware” on page 61 for instructions on updating the firmware. •...
Page 81 Appendix C Recommended instrument settings Run History Run History Select Run History to display the entire list of runs performed by the instrument. • Select a particular file to view the details of that run. • Select Export to save the run details to a USB memory device, or Delete to delete the the run history file.
Page 82 Appendix C Recommended instrument settings Run History – Delete a run report – Export a run report Neon NxT Electroporation System User Guide ™...
Page 83 Specifications Product specifications Operating power: 100–240 VAC, Frequency 50/60 Hz, 270 VA Output: 0.5-2.5 kV Pulse width: 1-100 ms Maximum duty cycle: Charging time: Maximum 6 seconds Altitude: Up to 2,000 meters Operating temperature: 15℃ to 30℃ Maximum relative humidity: Up to 80% Degree of protection: IPX0 Protective earthing:...
Page 84 Appendix D Specifications Networking requirements Networking requirements Configure the system behind a firewall. If outbound traffic is limited, the following firewall exceptions are required to support system features: Firewall lexception requirements Port Purpose Applies to… *.instrumentconnect.com outbound 443 To support instrument Thermo Fisher Connect ™...
Page 85 Related products Accessory products Additional products The following products are for use with the Neon NxT Electroporation System and are available ™ separately. For more information, go to thermofisher.com or contact Technical Support. Catalog numbers that appear as links open the web pages for those products. Product Quantity Catalog no.
Page 86 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. Xenon SingleShot Electroporation 6 chambers A50305 ™ ™ Chamber Xenon MultiShot Electroporation 1 cartridge A50306 ™ ™ Cartridge Xenon Electroporation Buffer 100 mL A4997901 ™ ™ A4997902 Xenon Genome Editing Buffer 100 mL A4998001 ™...
Page 87 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. Dynabeads Untouched Human T Cells Kit 1 kit 11344D ™ ™ Dynabeads Human T-Activator CD3/CD28 for 2 mL 11131D ™ T cell expansion and actviation 0.4 mL 11161D 5 × 2 mL 11132D Dynabeads Mouse T-Activator CD3/CD28 for 0.4 mL 11452D ™...
Page 88 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. TrueCut Cas9 Protein 2.5 mg A45220 ™ ™ 5.0 mg A45221 TrueGuide sgRNA Positive Control, HPRT1 3 nmol A35524 ™ (human) TrueGuide sgRNA Negative Control, non- 3 nmol A35526 ™ targeting 1 TrueGuide sgRNA Positive Control, AAVS1 3 nmol A35522 ™...
Page 89 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. LentiArray Lentiviral sgRNA custom thermofisher.com/us/en/home/life- ™ science/genome-editing/geneart- crispr/crispr-libraries/lentiarray- crispr-libraries/lentiarray- grnas.html TrueGuide gRNA custom thermofisher.com/trueguide ™ TrueGuide sgRNA Positive Control, Rosa26 3 nmol A35525 ™ (mouse) TrueGuide sgRNA Positive Control, CDK4 3 nmol A35523 ™...
Page 90 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. StemPro hESC SFM 1 kit A1000701 ™ KnockOut Serum Replacement 500 mL 10828028 ™ GlutaMAX -I Supplement 100 mL A1286001 ™ ™ KnockOut DMEM/F12 500 mL A1370801 ™ ™ Fetal Bovine Serum, dialyzed, US origin 500 mL 26400044 Products for cell analysis...
Page 91 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. QuantStudio 7 Pro Real-Time PCR System 1 system A43163 ™ A43170 A43183 QuantStudio Absolute Q Digital PCR System 1 system A52627 ™ ™ BigDye Terminator v3.1 Cycle Sequencing Kit 1 kit 4337454 ™...
Page 92 Appendix E Related products Accessory products (continued) Product Quantity Catalog no. ProcartaPlex Multiplex Immunoassay Kits custom thermofisher.com/us/en/ ™ home/life-science/antibodies/ immunoassays/procartaplex- assays-luminex/procartaplex- immunoassays.html ProQuantum High-sensitivity Immunoassay custom thermofisher.com/immunoassays/ ™ Kits elisa/query/proquantum QuantiGene Plex Gene Expression Assays custom thermofisher.com/us/en/home/ ™ life-science/gene-expression- analysis-genotyping/quantigene- rna-assays/quantigene-singleplex- assay/quantigene-singleplex- assay-ordering eBioscience Human Regulatory T Cell...
Page 93 Safety Safety information Follow the instructions in this section to ensure safe operation of the Neon NxT device. The Neon ™ ™ NxT Electroporation System is designed to meet EN61010-1 Safety Standards. To ensure safe, reliable operation, always operate the Neon NxT Electroporation System according to the instructions in this ™...
Page 94 Appendix F Safety Informational symbols (continued) Symbol and description WEEE (Waste Electrical and Electronic Equipment) symbol indicates that this product should not be disposed of in unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE. This instrument meets European requirement WEEE Directive 2012/19/EU.
Page 95 Appendix F Safety Informations de sécurité Informations de sécurité Suivez les instructions de cette section pour vous assurer d’utiliser l’appareil Neon NxT en toute ™ sécurité. Le Neon NxT Electroporation System est conçu pour répondre aux normes de sécurité ™ EN61010-1. Pour assurer un fonctionnement sûr et fiable, utilisez toujours le Neon NxT Electroporation ™...
Page 96 Appendix F Safety Informational symbols (continued) Symbol and description ON (MARCHE) (alimentation) OFF (ARRÊT) (alimentation) Protection par la mise à la terre (masse) La marque CE est un symbole indiquant que le produit est conforme à toutes les dispositions applicables de la Communauté européenne pour lesquelles ce marquage est obligatoire. L’utilisation du Neon NxT Electroporation System est soumise aux conditions décrites dans ™...
Page 97 Appendix F Safety Environmental requirements Environmental requirements Condition Acceptable Range Installation site Indoor use only Electromagnetic Do not use this device in close proximity to sources of strong electromagnetic interference radiation (for example, unshielded intentional RF sources). Strong electromagnetic radiation may interfere with the proper operation of the device. Altitude Maximum of 2000 m (6500 ft.) above sea level Operating conditions...
Page 98 Appendix F Safety Safety and electromagnetic compatibility (EMC) standards Safety and electromagnetic compatibility (EMC) standards The instrument design and manufacture complies with the following standards and requirements for safety and electromagnetic compatibility. Safety Reference Description EU Directive 2014/35/EU European Union “Low Voltage Directive” IEC 61010-1 Safety requirements for electrical equipment for measurement, control, and laboratory use –...
Page 99 Appendix F Safety Electrical safety Environmental design standards Reference Description EU Directive 2012/19/EU European Union “WEEE Directive”—Waste electrical and electronic equipment EU Directive 2011/65/EU European Union “RoHS Directive”—Restriction of hazardous substances in electrical and electronic equipment Commission Delegated Directive (EU) 2015/863 Regulation EC 1907/2006 European Union “REACH Directive”—Registration, Evaluation, Authorisation and Restriction of Chemicals SJ/T 11364-2014...
Page 100 Appendix F Safety Electrical safety • Ensure that the connections with information technology equipment, e.g. PCs or other industrial computers, comply with the IEC60950-1/EN60950-1, IEC61010-1/EN 61010-1, or IEC 62368-1/EN 62368-1 standards that apply in each case. • Unless expressly permitted, never remove the cover or any part of the housing while the product is in operation.
Page 101 Appendix F Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: · Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials.
Page 102 Appendix F Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases.
Page 103 Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •...
Page 104 Neon NxT Electroporation System_UG_MAN0026677-v2-GUID-42F0C04D-C894-49E6-9BE2- F1E0DA163DEF-2023/08/01 21:13:27 en 22:11:38.443+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 22 October 2024...

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