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157/159 UV-VIS Detectors
User's Guide
Gilson, Inc.
| PO Box 620027 | Middleton, WI 53562-0027, USA
Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451
Gilson S.A.S.
| 19, avenue des Entrepreneurs | BP 145 | F-95400 Villiers-le-bel, FRANCE
www.gilson.com
| sales@gilson.com | service@gilson.com | training@gilson.com
LT1037-03
©2015 Gilson, Inc. | All rights reserved.
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Summary of Contents for Gilson 157
- Page 1 | PO Box 620027 | Middleton, WI 53562-0027, USA Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145 | F-95400 Villiers-le-bel, FRANCE www.gilson.com | sales@gilson.com | service@gilson.com | training@gilson.com LT1037-03 ©2015 Gilson, Inc. | All rights reserved.
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Page 3: Table Of Contents
Pièces détachées ............... . Sécurité-4 Gilson 157/159 UV-VIS Detectors User’s Guide... - Page 4 Run Results Window ..............3-9 Gilson 157/159 UV-VIS Detectors User’s Guide...
- Page 5 Unit End-of-Life ................5-6 Gilson 157/159 UV-VIS Detectors User’s Guide...
- Page 6 Standby ..................B-15 Gilson 157/159 UV-VIS Detectors User’s Guide...
- Page 7 For your own safety, read this user's guide and always observe the warnings and safety information on the detector and in this user's guide. The 157/159 UV-VIS Detectors are suitable for high-pressure liquid chromatography and are intended to be used in a laboratory environment by trained technical personnel.
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Page 8: Safety
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in equipment damage Prohibited Use Explosion hazard if the device is used in potentially explosive atmospheres without appropriate protective equipment! Safety-2 Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 9: Solvents
To avoid damage from leaks, always place solvent bottles in a solvent tray on the device. Leaks and Clogged Tubing Regularly check for leaks and clogged tubing. Test back pressure without a column. Gilson 157/159 UV-VIS Detectors User’s Guide Safety-3... -
Page 10: Protective Measures
You are only permitted to perform the maintenance tasks described in this user's guide. If additional maintenance is required, contact your local Gilson representative. Personal Safety Equipment When working with the detector, take measures according to lab regulations and wear protective clothing, such as safety glasses with side protection, protective gloves, and a lab coat. -
Page 11: Sécurité
Pour votre sécurité, lisez attentivement ce manuel, et respectez les informations et avertissements relatifs à la sécurité indiqués sur l‘appareil et dans ce guide. Les détecteurs 157/159 UV-VIS sont adaptés à la chromatographie liquide haute pression et sont destinés à être utilisés dans un environnement de laboratoire, par un personnel technique qualifié. -
Page 12: Symboles Et Signes
AVIS: indique une situation potentiellement dangereuse qui, si elle n'est pas évitée, peut entraîner des dégâts matériels Restriction d’utilisation Risque d'explosion si l'appareil est utilisé en atmosphère potentiellement explosive sans équipement de protection approprié ! Sécurité-2 Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 13: Solvants
Pour éviter les dommages causés par des fuites, placez toujours les bouteilles dans un plateau à solvants sur l‘appareil. Fuite et obstruction des tubulures Assurez-vous régulièrement de l'étanchéité et de la non obstruction des tubulures. Testez la contre-pression sans colonne. Gilson 157/159 UV-VIS Detectors User’s Guide Sécurité-3... -
Page 14: Mesures De Protection
Vous n‘êtes autorisés à effectuer que les tâches de maintenance décrites dans ce guide. Si cela s‘avérait insuffisant, merci de contacter votre représentant Gilson. Équipement de protection individuelle Lorsque vous travaillez avec le détecteur, observez les mesures relatives au travail en laboratoire et portez un équipement de protection adapté... -
Page 15: Introduction
• Technical Specifications • Customer Service The 157 UV-VIS Detector is a single, variable wavelength HPLC spectrophotometer detector for routine HPLC applications, including fast LC methods. This robust UV detector features a highly flexible and compact design. The detector offers a variable wavelength range of 190–750 nm and provides a competitive advantage for biological samples. -
Page 16: Unpacking
Grip the detector and lift it out of the packaging. Remove the protective film from the touchscreen. It prevents scratches during shipping. Standard Equipment • 157 UV-VIS Detector or 159 UV-VIS Detector with test cell • Power cord (110V) •... -
Page 17: Technical Specifications
Technical Specifications Please be aware of the following before operating the detector. Changes or modifications to this device not expressly approved by Gilson could void the warranty. The instrument complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This instrument may not cause harmful interference, and (2) this instrument must accept any interference received, including interference that may cause undesired operation. -
Page 18: Customer Service
Technical Specifications - 157/159 UV-VIS Detectors (Page 2 of 2) Customer Service Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following types of assistance: sales, technical support, applications, and instrument repair. If you need assistance, please contact your local Gilson representative. Specific contact information can be found at www.gilson.com. -
Page 19: Installation
Installation This chapter takes you through the steps for setting up your detector, which includes: • Operating Environment • Rear Panel Connections • Plumbing Connections • Flow Cell Installation Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 20: Operating Environment
1-3 for more information). If the detector has been very cold, it should stand for at least 24 hours in its new environment in order to allow any condensation on the surfaces to evaporate. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 21: Rear Panel Connections
To make the Ethernet connection to the instrument, a router and Ethernet cables are needed. Note: The router is not available from Gilson, Inc. 1 Connect power to the router Connect the power supply to the router and then connect the power supply to a power source. -
Page 22: Events And Remote Control Terminal Strip
Electrostatic discharge hazard, damages to system, device, or components can occur. Wear an electrostatic discharge (ESD) grounding strap. Short-circuit hazard. Turn off the detector and disconnect the power cord before connecting it to the pin header. Tools Depressor tool Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 23: Integrator Connector
0 to 5V • DAC 20 bit • scalable • adjustable to offset Power Cord Connection Locate the appropriate power cord for the voltage. Use the power cord to connect the detector to a power source. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 24: Plumbing Connections
(adjustable) Standard Flow Cell Fittings (Stainless Steel) 14100013 PEEK 0.05 mm 0.16 μL Figure 2-4: Standard Flow Cell Fittings (PEEK) *Requires the Flow Cell Adapter Kit (part number 14100011) for connecting to 1/16" tubing Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 25: Inlet Tubing Connection
This fitting accommodates most existing female column outlets. a) Insert the fitting onto the tubing. b) Screw the fitting into the column outlet and then finger tighten. Figure 2-6: Inlet and Outlet Tubing Connection Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 26: Outlet Tubing Connection
Use the appropriate coupler to make the connection. Universal couplers and fittings are included in the plumbing package. • a waste container if the column effluent does not need to be saved. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 27: Flow Cell Installation
(0.75 mm), resulting in a shortened optical pathlength of 1.25 mm. To shorten to 0.5 mm, the PEEK spacer on the other side of the flow cell must also be removed. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 28: Flow Cell Installation
Continue to hold knurled-head the flow cell securely. screw carriage Slide the carriage back to the detector. Screw the knurled-head screws Figure 2-8: Flow Cell Installation back into the cover plate and then tighten. 2-10 Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 29: Operation
Operation TRILUTION® LC Software provides software control of the 157 Detector or 159 Detector for setup and operation of the detectors in a chromatographic run. For more information about TRILUTION® LC Software, refer to its user’s guide and on-line help. -
Page 30: Front Panel
Installation, to make all rear panel and plumbing connections. Ensure that the detector and the router are connected to a power source. Figure 3-2: Start Up Display Example (157 UV-VIS Detector) Switch the detector ON using the switch on the rear panel. •... -
Page 31: Turn Lamp On
Tasks are filtered by instrument name. Drag and then drop the Turn Lamp On task into the workspace. Click OK. Turn Lamp On task Figure 3-5: Turn Lamp On Task Click Run on the Manual Control window. Note: The detector requires a 15–30 minute warm-up period to achieve a stable baseline. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 32: Detector Control From Trilution Lc
Configuration On the Configuration tab in the Method Builder, tell the software that a 157 Detector or 159 Detector will be used and how many data channels on the detector will be used. In the Method Builder, select the Configuration tab. - Page 33 The Use check box for Data Channel 1 defaults selected. There is one data channel available for the 157 Detector (labeled 157 Channel 1), and there are four data channels available for the 159 Detector (labeled 159 Channel 1 through 159 Channel 4).
- Page 34 Operation 157 Detector Settings This task sets the wavelength for the 157 Detector. 157 Detector Settings Property Name Description Default Value Task Property Page Detector The detector that the task will affect. Detector Wavelength The monitor wavelength. Valid range is 190–750 nm.
- Page 35 Optionally, use error handling to check the status of the instrument. Run a stop method to shut down the system if an error is encountered. Error Description Instrument Error This error results when any scheduled command fails to execute or if the software loses communication with an instrument. Gilson 157/159 UV-VIS Detectors User’s Guide...
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Page 36: Analysis
Ensure that the lamp is on before collecting any data, either during your run or while monitoring the baseline in Manual Control. After setting up the sample list, click Run in the Application Run window to begin executing the steps in the run. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 37: Results
Double-click on a Run Name to display the Run Results window. Run Results Window The Run Results window enables viewing chromatogram plots and analysis information for collected data. To view chromatogram results, preview the report. Figure 3-6: Run Results Window Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 38: Maintenance
You are only permitted to perform the maintenance tasks described in this user's guide. If additional maintenance is required, contact your local Gilson representative. All smooth surfaces of the device can be cleaned with a mild, commercially available cleaning solution, or with isopropanol. -
Page 39: Flow Cell Maintenance
If the cleaning does not have the desired effect, clean the lens (refer to Clean the Lens in the Flow Cell on page 4-3) or fiber optics (refer to Clean the Fiber Optics in the Flow Cell (Adjustable Pathlength Flow Cell Only) page 4-4). Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 40: Clean The Lens In The Flow Cell
Assemble the flow cell and make sure that the new PTFE seal does not interrupt the light path. Tighten the threaded ring with the hexagonal spanner to avoid damage to the lens. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 41: Clean The Fiber Optics In The Flow Cell
12 Insert the stainless steel cover. 13 Use the Allen wrench to carefully tighten the threaded ring. When tightening the threaded ring, the rod-shaped fiber optics is pushed into the correct position in the cell Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 42: Replace The Flow Cell
Insert the new flow cell into the flow cell carriage. Continue to hold the flow cell securely. Slide the carriage back to the detector. Screw the knurled-head screws back into the cover plate and then tighten. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 43: Replace The Flow Cell Fittings
0.05 mm 49041078 FERRULE, 1/16" ANTI-TWIST, PEEK (M250) 49041079 NUT, 1/16" ANTI-TWIST, PEEK (M655) Figure 4-7: Standard Flow Cell Fittings (PEEK) *Requires the Flow Cell Adapter Kit (part number 14100011) for connecting to 1/16" tubing Gilson 157/159 UV-VIS Detectors User’s Guide... - Page 44 14100006 0.5 mm 14002031 DYNASEAL CONNECT SYS, 1/16" OD, 157/159 14100009 3 mm 14002028 FERRULE, SS, 1/16" OD, SPLIT GRV 157/159 14002029 FERRULE, POLYMER, 1/16" OD, 157/159 14002030 NUT, DYNASEAL, SS, 1/16" OD, 157/159 Stainless steel fitting Tubing Split-grooved ferrule with internal threading...
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Page 45: Replace The Inlet And/Or Outlet Tubing
Disconnect the tubing from the flow cell and, if necessary, from the system. Connect the fittings on the replacement tubing to the flow cell. Finger tighten the fitting. Connect the other end of the tubing, if necessary. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 46: Lamp Replacement
Protective Measures - Wear Protective Gloves Wear protective gloves! Do not touch the glass body with hands. Deposits can falsify measuring results and can damage the lamp. Required Tools • 2 mm Allen wrench • Screwdriver Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 47: Overview
Remove the two 2 mm Allen screws (labeled A and C in the figure below) that secure the cover to the detector. Push the cover back and then lift up to remove. Legend Allen screw Figure 4-9: Remove Allen Screws Cover Allen screw Figure 4-10: Remove Cover 4-10 Gilson 157/159 UV-VIS Detectors User’s Guide... - Page 48 Figure 4-12: Turn Locking Ring to Disconnect Lamp screw Remove Lamp Remove the two screws that secure the lamp in the lamp pod. Remove the lamp from the lamp pod. screw Figure 4-13: Remove Screws Gilson 157/159 UV-VIS Detectors User’s Guide 4-11...
- Page 49 Figure 4-15: Install Screws Plug in the connector and then turn the locking ring until the connector is secured. locking ring Figure 4-16: Connector with Locking Ring 4-12 Gilson 157/159 UV-VIS Detectors User’s Guide...
- Page 50 Place the cover on the detector. Secure the cover to the detector using the two 2 mm Allen screws. Legend Figure 4-17: Place Cover Allen screw Cover Allen screw Figure 4-18: Install Allen Screws Gilson 157/159 UV-VIS Detectors User’s Guide 4-13...
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Page 51: Troubleshooting
Troubleshooting This chapter provides information on the following topics: • Troubleshooting • Solvent and Buffer Compatibility • Repair and Return Policies Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 52: Device Will Not Turn On
Raise the fraction collector to a level higher than the flow cell. • The lamp intensity may be weak. Refer to Lamp Replacement on page 4-9. • Check flow cell for contamination. Refer to Flow Cell Maintenance on page 4-2. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 53: Baseline Drift
After the connections have been verified, use the procedure below to confirm communication. Reboot the computer. Turn off power to the router and the detector. Wait five minutes. Turn power on to the router. Turn power on to the detector. Start TRILUTION® LC Software. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 54: Solvent And Buffer Compatibility
• Water * valid for the specified temperature range Liquids can cause damage to the detector. Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 55: Efficient Hplc Separations
Use Filtered Solvents Use ultra-pure, filtered solvents for HPLC – gradient grade. Filtration of substances under analysis. Use of inline filters. Check for Clogged Tubing Regularly check for clogged tubing. Test back pressure without a column. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 56: Repair And Return Policies
Repair and Return Policies Before Calling Us Your local Gilson representative will be able to serve you more efficiently if you have the following information: • the serial number and model number of the instruments involved. The serial number is located on the rear panel. -
Page 57: Replacement Parts
FLOW CELL UV, 0.5MM 1/16 SS, 157/159 14100007 FLOW CELL UV, 0.5MM 1/16 PEEK, 157/159 14100008 FLOW CELL UV, 10MM 10μL 1/16 SS, 157 14100009 FLOW CELL UV, 3MM 2μL 1/16 PEEK, 157/159 14100010 FLOW CELL UV, 2MM 1/8 SS, 157/159, 0.5-2... -
Page 58: Inlet/Outlet Tubing And Fittings
Back pressure regulator (75 psi), stainless steel, for flow rates up to 10 mL/min 4320302 Allen wrench, 3 mm 7080318116 PWR CORD, EURO 250VAC 2.5A IEC320-C5 7080318117 PWR CORD, UL CSA 125VAC 10A IEC320-C5 14100005 SHELF, 157/159 DETECTOR Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 59: Front Panel Control
Front Panel Control Front panel control is available from the touchscreen. To use front panel control when the detector is under remote control, reboot the computer, then turn the detector off and then on again. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 60: Touchscreen
Touch briefly to move up one level Go to device status display Press and hold for 2 seconds Go to Main menu Touch to go to the Main menu Save the entry Touch to save the entry Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 61: Graphical User Interface Structure
Program: Device programming • Link: Creating combination programs • Scan: Wavelength scan (159 UV-VIS Detector only) • Setup: Device presets • GLP: Device status displayed according to good laboratory practice (GLP) • Standby mode Gilson 157/159 UV-VIS Detectors User’s Guide... - Page 62 Front Panel Control Figure B-1: User interface structure (1/2) Figure B-2: User interface structure (2/2) Gilson 157/159 UV-VIS Detectors User’s Guide...
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Page 63: Status Display Settings
Events are electric signals that are effective on the terminal strip events and remote control and are used to control the detector and other devices. Up to four events can be activated by touching the gray Event button. Autozero Touch Autozero to perform an autozero scan. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 64: Main Menu
Enter the event settings. Touch New to specify the time. (Diagram B). Touch to go to a higher level, or hold for two seconds to go to the Main menu. Figure B-6: Creating a Program Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 65: Creating Program Lines
(Diagram A). Touch to interrupt the program. Touch to stop the program. Touch to repeat the program (Diagram B). Touch to exit the loaded program. Figure B-8: Running a Program Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 66: Changing A Program
The WakeUp control mode can be started with WU > load after answering the security query “load wakeup program?”. The screen will display the characters WAKEUP, as well as the detector’s wake-up time and the current time. The screen turns on the power saving mode. Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 67: Link Menu
Select the desired option for wait (Diagram C). Touch to go to a higher level, or hold for two seconds to go to the Main menu. Figure B-13: Creating a Link Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 68: Executing A Link
Touch the desired link number and then Delete to delete the link. Confirm the query. Touch to go to a higher level, or hold for two seconds to go to the Main menu. Figure B-14: Deleting a Link B-10 Gilson 157/159 UV-VIS Detectors User’s Guide... -
Page 69: Scan Menu (159 Uv-Vis Detector Only
Touch Autozero to carry out the zero balance of the measuring signal. Touch Scan Start button to start the scan. Touch Scan Out to output the scan data to the selected integrator output (RCA port on the back of the device). Gilson 157/159 UV-VIS Detectors User’s Guide B-11... -
Page 70: Setup Menu
Figure B-19: Setup > Lamps T-const. Here a time constant can be selected from prescribed values from 0.1 s to 10.0 s, to smooth the analog output signal. Figure B-20: Setup > T-const. B-12 Gilson 157/159 UV-VIS Detectors User’s Guide... - Page 71 100 nm/V. The scaling can be changed with the number keys that appear after touching the field. Note: For optimal linearity, a scaling of 100 nm/V is recommended. Gilson 157/159 UV-VIS Detectors User’s Guide B-13...
- Page 72 Figure B-27: Setup > Event Setup > Date Touch the gray buttons next to Date and Time to set the date and time for the detector. Figure B-29: Setup > Date B-14 Gilson 157/159 UV-VIS Detectors User’s Guide...
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Page 73: Glp Menu
The return from standby mode is made by touching the touchscreen. The standby lamp goes out, and the device behaves as it would if turned on with the power switch. No calibration takes place, as the set wavelength has been fixed Gilson 157/159 UV-VIS Detectors User’s Guide B-15...
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