Setting Up The System - Merck Milipore Cellicon Perfusion Solution for Process Development User Manual

Cellicon Perfusion Solution for Process Development User Guide
TM

Setting Up the System

The system should be set up close to the bioreactor, with cords behind the holder. The
controller screen should be easily accessible to the user.
Connecting the Component Cables
Carefully remove all the system components from the packaging.
Connect the components at the rear of the controller as shown below.
USB Second
Flowmeter
(Optional)
USB
Flow
Analog In
24 V --- 0.5 A Out
Vent
Perfusate (Permeate)
Pressure Sensor
Once connected to the rear panel, connect the cables to the components as listed here:
Cable Color
Gray
Black
When all cables have been connected, connect the power cord to the power source.
Feed Pressure Retentate
Sensor Pressure
Pres. 1 Temp.
LAN
Press. 2 Remote
RS485
Press. 3
24 V --- 0.5 A Out
Primary
Flowmeter
Primary flowmeter
Pump
Drive
Power Out
24 V --- 0.5 A In
PLC
Power In
Connection
Power in (24 V)
Installing the Filter
Refer to System Components
reference.
Prior to installation, there may be a slight
kink in the feed tubing line between the filter
and the pressure sensor. This is cosmetic and
does not impact the function of the system.
It should self-resolve by laying the tubing
inside the flow meter clamp.
1. Carefully remove the filter and tubing
assembly from the bags, and remove the
assembly from the backing card.
2. Slide the device into the slot on top of the
holder.
3. Open flowmeter clamp (located at the center of
the holder) and lay tubing inside.
4. Close the flowmeter, ensure the tubing is not
pinched or kinked, then clamp it.
5. Place the pump head into the pump drive by
aligning the outlet barb on the pump head
with green coloring on pump drive. Pull pin
out and push pump head in place. Rotate
pump head clockwise so barb is located over
the red coloring and release pin.
6. Connect the three pressure cables from
the control box to the sensors on the
assembly as follows:
Press. 1 to the feed line
Press. 2 to the retentate line
Press. 3 to the perfusate (permeate) line.
Connecting the
Bioreactor
Use a tubing welder to connect:
• The cell retention filter feed line to
the device inlet line of the bioreactor,
ensuring the line is submerged, and
below the sparger.
• The cell retention filter retentate line to
the device outlet line of the bioreactor.
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