Troubleshooting - Merck Milipore Cellicon Perfusion Solution for Process Development User Manual

Cellicon Perfusion Solution for Process Development User Guide
TM
5. Locate a new filter and place it in the
holder.
6. Use a tubing welder to connect the
new filter to the tubing lines that were
designated for feed, retentate, and
permeate.
7. Unclamp the feed and retentate lines, but
ensure that the perfusate (permeate) is
clamped.
8. Prime the system again. If using a
peristaltic pump, run at a low flow rate to
avoid causing shear damage to the cells
in the line.
9. Restart the recirculation pump by
selecting on the main
START PUMP
menu. Ensure that the flow rate has
reached the desired setpoint.
10. Restart the perfusate (permeate) pump.

Troubleshooting

System Troubleshooting
The following chart can be used to help identify issues related to system operation.
Symptom
No power Plug in system and press power button
System Connect cables from component to controller
component
appears light grey
Install correct software
and does not read
values
Plug in component cables
Pump alert: Place pump head into motor
Component is in
an alarm state, Flow alert: Place tubing into flowmeter and
appears red or prime tubing
yellow
Adjust flow alarm ranges
Follow instructions provided for specific alert
Assembly does Follow order of operations for installation found
not fit into holder in user guide
Pull out locking pin during installation and move
barb from covering green area to covering red
Pump head does
area
not fit into motor
Place pump head facing upwards
Check login credentials and user permissions
System does not
Disable "When LAN Accessibility is enabled,
allow changes
console control is disabled" setting under
Settings->External Interfaces->LAN Accessibility
Use external peristaltic pump to start flow
Choose peristaltic pump appropriate for the
tubing size
System will not
prime
Ensure dip-tube is open and submerged in liquid
No pinching or kinking of tubing lines, welds are
open
Corrective Action
20