Merck Milipore Cellicon Perfusion Solution for Process Development User Manual page 21

Cellicon Perfusion Solution for Process Development User Guide
TM
Symptom
Check for cuts, cracks, or damage to assembly
Ensure system pressure is less than 5 psi
System leak
Ensure flowmeter clamp does not pinch the
tubing
Ensure cable connections are good
Proper installation of P1 on Feed, P2 on
Unexpected or
retentate, and P3 on permeate
unstable pressure
Perform zero adjustment prior to run, after
readings
priming
Clear air from the sensor
Ensure cable connections are good
Clean flowmeter and tubing with IPA
Close clamp around tubing and ensure
flowmeter clamp does not pinch the tubing
Ensure pump is running and flow is moving
Unexpected
Prime tubing in flowmeter, remove air bubbles
or unstable
Choose correct calibration set in system settings
flowmeter
(tubing type, temperature)
readings
Perform zero adjustment at the correct
temperature, prior to run and after priming
Re-zero sensor
Check flowmeter calibration against reference
flowmeter
Re-prime tubing to remove air bubbles
Remove pump head from pump to clear air from
inside
Check for tubing pinch or kink
Pump unable to
achieve target
Check for filter fouling (high P1 with low P2)
flow rate
Check dip-tube placement and ensure it does
not pull air from sparger
Check system mode, "flow control" vs "speed
control"
No flow through
Ensure permeate line is not clamped or pinched
permeate
Corrective Action Symptom
Check that crossflow rate is at setpoint
Check system mode, "flow control" vs "speed
control"
Ensure maximum flux is within recommended
Early filter fouling
range
(High P1 or low
P3)
Swap out filter
Check flowmeter calibration setting is correct
Check flowmeter calibration against reference
flowmeter
Check bioreactor pressure (ex. vent filter
clogging)
System pressure
Check for tubing pinch or kink on retentate line
increase (High P1
Check flowmeter calibration setting is correct
and P2)
Check flowmeter calibration against reference
flowmeter
Check flow rate and pump RPM are in
recommended range
Check flowmeter calibration setting is correct
Check for cell loss in permeate line
Cell growth issues
in bioreactor
Prime filter during media hold only
Re-prime system to remove air bubbles
Check flowmeter calibration against reference
flowmeter
Cells found in
Swap out filter
permeate
Adjust alert settings
Adjust alarm limits
Unexpected or
undesired alerts
Perform factory reset
Follow recommendations provided for each alert
Ensure USB is inserted during run
Data logging is Ensure USB drive has adequate space
not saved
USB is not removed until data logging is turned
off and software indicates it is safe to remove
Corrective Action
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