Shutdown; Planning For A Run; Preparation - Merck Milipore Cellicon Perfusion Solution for Process Development User Manual

Cellicon Perfusion Solution for Process Development User Guide
TM

Shutdown

When the power button is pressed a
confirmation pop-up will appear. After
confirmation, the controller software initiates
the shutdown.
Shutdown Confirmation
When the main power button is pressed
for more than 4 seconds, the hardware
shutdown is triggered, the pump turns OFF
and the controller immediately shuts down.
Planning for a
Run
If possible, the Cellicon Perfusion filter
TM
should be attached to the bioreactor and
the recirculation pump should be turned
on during the media hold phase, before
cells have been added to the reactor. This
will eliminate the possible introduction of
cell shear when the system is primed (see
Priming).

Preparation

Equipment Needs
The following user supplied items may be
required to execute a perfusion process:
• Assembled and sterilized bioreactor, up to
3L working volume
• Control system for the bioreactor
• Welder for 1/8 in. ID x ¼ in. OD weldable
(Advantaflex) tubing to attach the filter
• Peristaltic pump for the perfusate
(permeate)
• Peristaltic pump for priming
• Method to control the liquid level in the
bioreactor to maintain a constant volume
• Power outlet for plugging in the cell
retention system (100-240V, 1.7A max,
50/60hz)
• Sterile collection vessels to contain the
fresh media and collect the spent media
during the run.
• USB drive for data collection
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