Merck Milipore Cellicon Perfusion Solution for Process Development User Manual page 17

Cellicon Perfusion Solution for Process Development User Guide
TM
P&ID for N-1 Perfusion Process.
Mobius 3L Bioreactor
Cellicon TM
Filter
Preparing the
Bioreactor
Connect the filter to the bioreactor by
welding Advantaflex tubing on the assembly.
To optimize process efficiency, reduce
the holdup of cell culture volume in the
recirculation loop outside of the bioreactor
by shortening the tubing length between the
bioreactor and the filter assembly. Tubing ID
cannot be smaller than 1/8-inch at any point
in the recirculation loop. If using a diptube,
ensure that the ID of the diptube is between
1/8-inch and 3/8-inch.
Place the inlet to the cell retention filter
below the liquid level of the bioreactor, so it
will not pull in any bubbles that have been
released from the sparger.
Note Any air or gas in the pump head will
negatively affect pump performance
and decrease the recirculation flow rate.
Mobius 3L Single-use Bioreactor
TM
When using the Mobius single-use 3L
TM
reactor, the inlet to the cell retention
filter should be welded to the harvest line
(bottom) of the bioreactor. The retentate line
should be welded to one of the submerged
ports that does not contain a check value
(see image X). This is the ideal orientation
for the Cellicon Perfusion filter because
TM
sparger will be less likely to pull bubbles in,
and priming the system is easier.
Other Bioreactors
The Cellicon Perfusion Solution for
TM
Process Development can be used with any
bioreactor, single or multi use, with a working
volume of 3L or less. On single use reactors,
choose the harvest line as the inlet to the
filter. On most glass reactors, use a harvest
tube that extends below the sparger, or add
a length of tubing that extends to the bottom
of the diptube below the sparger.
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