Laser Safety Instructions - Leica SP8 Manual

Laser Safety Instructions

• During operation of class 3B and class 4 lasers red warning lights have to be switched on
manually
• Red warning light at the door of the room, containing laser-based equipment, prohibits the
entrance
• Optical path of the laser beam at all setups has to stay intact and should never be
disassembled by a user. Users are never permitted to disconnect optical connections (pipes,
fibers etc), remove protective coverings or disassemble any parts of the setups, especially
those parts that are labeled with laser-warning signs.
• User has to make sure, that objectives mounts are blocked by objectives or light- blocking
plugs, before switching the system on or starting the work
• Any cleaning activities (objectives, stage cleanings) as well as changing of objectives or filters
have to be performed only after blocking of the laser light is ensured. This can be ensured by
closing the scanhead shutter or switching off the laser.
• Laser class-specific warnings at each setup have to be observed and considered
• Eye contact with direct beam of Class 3B laser, or eye contact with mirror reflection from class
3B laser, should be avoided at all times
• Eye or skin contact with direct or diffuse light of Class 4 laser, should be avoided at all times
• Laser safety goggles are situated at all workspaces and should be used in any situation where
potential contact of eyes with Laser light of the classes 3B or 4 is possible, according to the
previous two points
• Laser safety goggles have to be worn at all times of operation of Laser Class 4
• Laser safety goggles have to be worn at all times of operation laser Class 3 at the optogenetic
setups
• Laser safety goggles are assigned to each setup and matched to the corresponding laser
wavelengths. Matching laser safety goggles should be used at all times, and should not be
carried over between the setups
• Only one person is allowed to be in the corresponding compartment during Laser Class 4
operation and optogenetic setup operation
• Users are not allowed to wear any reflective objects (rings, watches etc) during laser operation
• Using of the equipment is only allowed after the introduction from a laser safety officer of IST
Austria. Introduction has to be done individually for each setup.
• Changing experimental conditions, that involves changes in the laser application, have to be
reported to the laser safety officer prior to the start of experiment
Users have to understand that any violations against the instructed rules and also withholding
information leading to safety hazards will ultimately result in denial of admission to all laser equipped
instruments at the IST Austria.