Izon qNano User Manual page 46

9 / Pore cleaning and storage
Once all data has been collected, do not leave the pore on the instrument.
Wash the pore before storing in a clean, resealable bag. Nanopores should
last in storage for years if they are properly cleaned and dried as below
before being packed away.
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IMPORTANT NOTES:
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46
Stretch the nanopore to 47 mm, remove the upper fluid cell, apply a
droplet of electrolyte to the top of the nanopore and replace the lower
fluid with fresh electrolyte.
Rinse the upper fluid cell with DI water and dry with compressed air.
Wipe the top of the nanopore dry with a lint free tissue, fit the upper fluid
cell and pipette in 35
Apply maximum pressure until you are satisfied that there are no
significant particle blockades present.
Repeat with DI water and ensure that the current goes to ~0 nA.
Remove the nanopore, rinse with DI water and dry with compressed
gas. Do the same with the upper and lower fluid cells, and make sure the
inside of the fluid cell cap is also clean and dry.
Re-assembled the fluid cell components on the qNano and put the clean,
dry nanopore in its ziplock bag.
Do not submerge the fluid cell – only rinse the fluid chamber.
Take care not to get any fluid in between the plastic and metal
connections in the fluid cell (as fluid trapped between components can
cause noise fluctuations and short circuits).
Always dry the fluid cell with compressed air after it has been cleaned.
Always ensure the fluid cell is completely dry before fitting back onto the
pore.
L of electrolyte.
µ
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