Izon qNano User Manual page 23

Optimising particle size resolution
The green zone assists users to achieve optimised measurement settings
for the quoted resolution range. Calibration particles must lie within the
green zone to ensure that the smallest particle can be detected. This can be
achieved by changing the applied nanopore stretch. The calculated location of
the green zone depends on:
—
—
The relative particle size (%) is an indication of particle size relative to the
nanopore for individual particles. Change the applied stretch to optimise
the relative particle size. At a constant stretch, a larger particle size would
produce larger blockades.
The green zone is only shown in the custom planner tool and is not available in
the classic capture tool.
Figure 8. A visual representation of the importance of the green zone and how particles are
excluded above and below.
www.izon.com
The smallest particle diameter (set in the resolution range)
The mean size of the calibration particle used. The software will show the
recommended size of calibration particles to use.
23