Izon qNano User Manual page 19

If concentration is calculated at a single pressure the software will calculate
a gradient based on the fitted line passing through the origin. This can
introduce significant errors, especially with smaller nanopores (NP200 and
below) where a large proportion of particles are driven through the nanopore
due to applied voltage, V.
Important
For accurate and precise results, the sample and calibration particles must
be recorded under identical system settings, which includes the electrolyte
characteristics, which affect the baseline current and blockade magnitude.
When possible, always use the same electrolyte.
Adjusting any of the parameters between the sample and calibration
recordings will affect blockade magnitude and particle rate, invalidating the
sample-calibration pairing.
Sample and calibration particles should be recorded at the same time – one
immediately after the other, or close together as possible if the system is stable
enough to record 2-3 samples before the system needs to be recalibrated.
www.izon.com 19