Izon AFC V2 Quick Start Manual

Q U I C K S TA R T G U I D E
AU T O M AT I C F R A C T I O N
C O L L E C T O R V 2 ( A F C )
This quick start guide only provides general operating instructions. For more detailed
information, you can download the AFC V2 User Manual at

Intended Use

The AFC is used to automatically collect fractions from qEV Isolation Columns. The
instrument is intended to be used in research laboratories by professional personnel
for research use only. For verification of the entire system, it is recommended that Good
Laboratory Practices (GLPs) be followed to ensure reliable analyses.

Components of the AFC System:

1. AFC Instrument with attached
Nozzle Set and Silicone Waste Tube
2. Nozzle Set (x3)
3. qEV Column Mount (x5)
4. Carousel Cover
5. Reversible Carousel
6. Carousel Plate
7. qEVoriginal Reservoir
The carousel is reversible and can be used in two orientations:
One side has seven large holes and uses 5 mL collection tubes. Use with qEV10 ONLY.
—
One side has thirteen small holes and uses either 1.5 mL or 2.0 mL collection
—
tubes. Use with qEVsingle, qEVoriginal, qEV1 or qEV2.
— qEV100 is not compatible with the AFC.
To complete a run on the AFC you will also need:
A qEV column, pipettes and tips, freshly prepared and 0.22 μm filter-sterilised buffer,
freshly prepared 0.5 M NaOH solution for column cleaning, 70% ethanol solution
for cleaning and a computer with Windows 10 OS for updating the AFC operating
firmware as required.
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8. 5.0 mL Collection Tube (x15)
9. 2.0 mL Collection Tube (x30)
10. 12 Volt Power Supply
11. Power Lead
12. USB 2.0 Type A to USB 2.0 Micro
Cable (male to male)
13. 10 g Calibration Weight
1