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QUICK START GUIDE
A U T O M AT I C F R A C T I O N C O L L E C T O R V 2
( A F C )
This quick start guide only provides general operating instructions. For more detailed
information, you can download the AFC V2 User Manual at
INTENDED USE
The AFC is used to automatically collect fractions from qEV columns. The instrument is
intended for use in research laboratories by professional personnel for research use only.
For verification of the entire system, it is recommended that Good Laboratory Practices
(GLPs) be followed to ensure reliable analyses.
COMPONENTS OF THE AFC SYSTEM:
1.
AFC Instrument with attached
Nozzle Set and Silicone Waste Tube
2.
Nozzle Set (x3)
3.
qEV Column Mount (x5)
4.
Carousel Cover
5.
Reversible Carousel
6.
Carousel Plate
7.
qEVoriginal Reservoir
The carousel is reversible and can be used in two orientations:
One side has seven large holes and uses 5 mL collection tubes.
—
—
One side has thirteen small holes and uses either 1.5 mL or 2.0 mL collection tubes.
Cannot be used with the qEV10.
—
qEV100 is not compatible with the AFC.
To complete a run on the AFC you will also need:
A qEV column, pipettes and tips, buffer (freshly prepared, 0.22 µm filtered, and degassed),
freshly prepared 0.5 M NaOH solution and 70% ethanol solution for column cleaning, and a
computer with Windows 10 for updating the AFC operating firmware as required.
www.izon.com
support.izon.com
8.
5 mL Collection Tube (x15)
9.
2 mL Collection Tube (x30)
10.
12 Volt Power Supply
11.
Power Lead
12.
USB 2.0 Type A to USB 2.0 Micro Cable
(male to male)
13.
10 g Calibration Weight
1
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Related Manuals for Izon AUTOMATIC FRACTION COLLECTOR V2
Summary of Contents for Izon AUTOMATIC FRACTION COLLECTOR V2
- Page 1 ( A F C ) This quick start guide only provides general operating instructions. For more detailed information, you can download the AFC V2 User Manual at support.izon.com INTENDED USE The AFC is used to automatically collect fractions from qEV columns. The instrument is intended for use in research laboratories by professional personnel for research use only.
- Page 2 Ensure the lower cap has been removed, insert the column into the column mount and carefully dock the column. Ensure that the Izon logo on the column is visible and the AFC can read the RFID tag.
- Page 3 When the sample has fully entered the loading frit, top up the column/reservoir with the correct amount of buffer to resume collection of the buffer volume. When the buffer volume has been collected, the carousel will move to position 1, collect the specified volume and then progressively move to collect each successive individual www.izon.com...
- Page 4 Switch off the AFC at the rear of the instrument. For more detailed instructions please see the AFC User Manual available at www.izon.com/resources support.izon.com/automatic-fraction-collector-v2-user-manual If your AFC needs replacement parts or repair please contact your regional Izon representative or open a support ticket at support.izon.com REV B...
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