Izon AUTOMATIC FRACTION COLLECTOR V1 Quick Start Manual

QUICK START GUIDE
AUTOMATIC FR ACTION COLLECTOR V1
(AFC)
This quick start guide only provides general operating instructions. For more detailed
information, you can download the AFC V1 User Manual at
INTENDED USE
The AFC is used to automatically collect fractions from qEV columns. The instrument is
intended for use in research laboratories by professional personnel for research use only. For
verification of the entire system, it is recommended that Good Laboratory Practices (GLPs) be
followed to ensure reliable analyses.
COMPONENTS OF THE AFC SYSTEM:
1. AFC Instrument with attached
Nozzle Set and Silicone Waste Tube
2. Nozzle Set (x3)
3. qEV Column Mount (x5)
4. Carousel Cover
5. Reversible Carousel
6. Carousel Plate
7. qEVoriginal Reservoir
The carousel is reversible and can be used in two orientations:
One side has seven large holes and uses 5 mL collection tubes.
—
— One side has thirteen small holes and uses either 1.5 mL or 2.0 mL collection tubes.
Cannot be used with the qEV10.
— qEV100 is not compatible with the AFC.
To complete a run on the AFC you will also need:
A qEV column, pipettes and tips, freshly prepared and 0.22 μm filtered and degassed buffer,
freshly prepared 0.5 M NaOH solution for column cleaning, 70% ethanol solution for cleaning
and a computer with Windows 10 for updating the AFC operating firmware as required.
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8. 5 mL Collection Tube (x15)
9. 2 mL Collection Tube (x30)
10. 12 Volt Power Supply
11. Power Lead
12. USB 2.0 Type A to USB 2.0 Micro Cable
(male to male)
13. 10 g Calibration Weight
1