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Related Manuals for Izon qEV
Summary of Contents for Izon qEV
- Page 1 AUTOMATIC FRACTION COLLECTOR (AFC) USER MANUAL www.izon.com...
- Page 2 Izon Science Ltd. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorisation of Izon Science Ltd.
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Page 3: Table Of Contents
Hazards ..............7 Setting up a run ..........23 Section 3: Introduction to the Automatic Fraction Collector (AFC) ........10 Mounting the qEV Column to the AFC Tower ............23 Overview .............. 10 Column Use Counter and Invalid Intended Use ............11 Columns .............. -
Page 4: Section 1: Definitions And Writing Conventions
This symbol indicates where special care should be taken Earth (ground) terminal Direct Current (DC) Voltage polarity of the jack Text that appears in pink is used to indicate buttons and SETUP sequences that are used on the AFC touchscreen www.izon.com... - Page 5 The supplied 10 g weight that is used to calibrate the load cell. Calibration Weight See Section 5. The size of the qEV column; the AFC will only work with Izon Column Type qEVsingle, qEVoriginal, qEV1, qEV2, and qEV10 columns.
- Page 6 The area from which fluid exits the qEV column. The entirety of the nozzle tip, a piece of silicon tubing, and the Nozzle Set adapter used to connect qEV columns is called the nozzle set. The volume immediately suceeding the buffer volume, containing Purified Collection Volume (PCV) particles of interest purified from the loaded sample.
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Page 7: Section 2: Safety And Hazards
< 0.1% sodium azide, which is potentially fatal if swallowed or in contact with skin. Please review the following guidelines and precautions prior to each use of the qEV column, especially if flushing with an antimicrobial buffer before storage (recommended). - Page 8 Remove immediately any contaminated clothing and wash before reuse. – Collect any spillage and dispose of appropriately. For more information, see the MSDS Documentation for Izon qEV columns: izon.com/sds Sodium Azide can be fatal if swallowed or in contact with skin. It can cause damage to neurological organs through prolonged or repeated exposure.
- Page 9 The AFC system contains no potentially hazardous chemical materials. However, when the appropriate qEV column is used on the AFC system, the user is potentially exposed to a dilute solution of Sodium Azide from the column and/or waste hose outlet. Refer to page 7 of this manual under the heading “Disposal of Biohazardous Material”...
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Page 10: Section 3: Introduction To The Automatic Fraction Collector (Afc)
(pink area in Fig. 1) and the volume of the fractions containing EVs. The buffer volume does not contain any EVs and is emptied into the central well of the carousel. The type of qEV column used in each run will determine the volume of liquid that is emptied into the central well. Once the buffer volume has been collected, the instrument will begin collecting a predetermined number of fractions of a given volume as set by the user. -
Page 11: Intended Use
The AFC is used to automate fraction collection of Izon’s qEV size-exclusion columns. The instrument is intended to be used in research laboratories by professional personnel for research purposes only. Neither the AFC nor the qEV column are intended for diagnostic purposes and should not be used to make treatment decisions. -
Page 12: Section 4: Instrument Specifications
The following components are provided in the box. Table 4: Components of the AFC System AFC Instrument with attached nozzle set and silicone waste tube Nozzle Set (x3) qEV Column Mount (x5) Carousel Cover Reversible Carousel Carousel Plate qEVoriginal Reservoir 5.0 mL Collection Tube (x15) -
Page 13: Layout Of The Reversible Carousel
Figure 2: Components of the AFC System Layout of the Reversible Carousel The carousel is reversible and can be used in two orientations: – One side has seven large holes and uses 5 mL collection tubes. This side is for use with the qEV10 ONLY. –... -
Page 14: Sample Buffer Recommendations
– qEV columns come equilibrated in filtered PBS containing < 0.1% w/v sodium azide. – To maintain the functionality of EVs, the buffer used with the columns should be of the same ionic strength and pH as the original sample buffer. -
Page 15: Section 5: Assembly And Setup Instructions
– Check the local supply meets the AC input requirement given in the specification. Izon instruments are only to be operated with Izon supplied leads and power supplies. Failure to use the correct power supply may result in invalid operation. -
Page 16: Aligning The Carousel
Turn on the instrument using the power switch at the rear. The screen will light up briefly showing the IZON logo and the carousel will rotate to its zero position. Ensure the valve is in place, as shown Figure 5... - Page 17 Following the screen, choose CAROUSEL > CALIBRATE Press the -/+ buttons (for fine alignment) and --/++ buttons (for coarse alignment) until the nozzle is positioned directly above the hole in the flush position as shown in Figure 7 below. Figure 7: The nozzle tip centred over the flush hole. Note that for the side of the carousel that holds 2 mL tubes, the flush hole will be on the right of the central well channel.
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Page 18: Calibrating The Load Cell
The user can choose whether to leave the tower and halo lights on or off through the Setup Menu. From the main menu, press SETUP > LIGHTING. On the following screen, select or deselect the tower or halo lighting modules. www.izon.com... -
Page 19: Section 6: Qev Specifications
SPECIFICATIONS Choosing the right qEV column to run will depend on the volume of the sample and the target isolation range of the analyte. Table 5: qEV Legacy Column Specifications Column Type qEVsingle qEVoriginal qEV2 qEV10 Optimal Sample Loading 150 µL... - Page 20 Table 6: qEV Gen 2 Column Specifications Column Type qEVoriginal qEV1 Optimal Sample Loading 500 µL 1 mL Volume1 Yes, up to 5 Yes, up to 5 Reusability times times Column Volume 8.5 mL 13.5 mL Buffer Volume2 2.5 mL 4.0 mL...
- Page 21 Isolation Ranges: All qEV columns are available in one of two isolation ranges, the qEV/35nm series and the qEV/70nm series. The qEV/35nm series of columns generally perform better when the target particle to be isolated is less than 110 nm in diameter, while the qEV/70nm series of columns generally perform better when the target particle to be isolated is greater than 110 nm in diameter (see Fig.
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Page 22: Section 7: Operating Instructions
Min Number of Fractions Max Fractions in a single Carousel Max Fraction Volume 2.0 mL 2.0 mL 2.0 mL 5.0 mL Min Fraction Volume 0.2 mL 0.5 mL 2.0 mL 5.0 mL Fraction Volume Increments 0.2 mL 0.5 mL www.izon.com... -
Page 23: Volume Size Selection
The AFC comes with a column mount for each compatible column type. Choose the column mount that matches the type of column you would like to use. Once a qEV smart column and column mount have been attached to the AFC, the column type is determined automatically and is displayed on the AFC touchscreen. - Page 24 Figure 10 below. Figure 10: A qEV original/70nm Legacy column loaded correctly onto the AFC. Ensure that the IZON logo is facing away from the AFC tower. Turn the column within the column mount until the type of qEV column used is displayed on the...
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Page 25: Column Use Counter And Invalid Columns
Column Use Counter and Invalid Columns The AFC instrument records the number of times that each smart column has been used. The columns are intended to be used up to five times. A warning indicator will be displayed when the recommended number of uses is approaching the maximum and after this number has been exceeded, as shown below in Figure 12. -
Page 26: Adjusting The Collection Schedule
0.284 mL collected. If you are collecting any buffer volume size, 0.284 mL will be collected on top of the chosen buffer volume. Press to save your parameters and return to the main menu. When you are ready to start your collection, press START COLLECTION and review your collection parameters. www.izon.com... -
Page 27: Setting Up The Carousel
Figure 16: The location of the “Start Collection” option. Press to proceed to carousel setup or to return to the main menu. Setting up the Carousel Once you have defined your collection parameters, you are ready to prepare the carousel for collection. Be sure to label collection tubes prior to loading them onto the carousel. -
Page 28: Initiating A Run
Press to continue. The next screen will ask you whether you would like to flush the column. We recommend flushing of all columns with fresh, filtered PBS buffer (See Section 6: qEV Specifications for information on buffer requirements). Press if you would like to flush the column and ensure that you have the appropriate amount of buffer for the column type. -
Page 29: Sample Loading And Initiating Collection
The next screen will ask you to have sufficient buffer available to run the column and to load the sample. For sample loading and buffer requirements for each column type, see Section 6: qEV Specifications. Ensure that there is no residual buffer left on the top of the column (remove any excess with a pipette) before adding the sample. -
Page 30: Completing A Run
Before turning off the AFC, rinse the AFC nozzle using DI water with the valve open. This will prevent salt crystallising in the tip of the nozzle while it is not in use. Switch off the AFC at the rear of the instrument. www.izon.com... -
Page 31: Section 8: Resources
RESOURCES Repair and Servicing of AFC Instrument – All replacement parts must be obtained via Izon Science Ltd. – Contact your regional Izon Science representative for replacement parts. We recommend that you save the box and packaging materials in case the instrument needs to be returned for servicing. -
Page 32: Updating The Afc Operating Software
Open the AFC Software Updater. The AFC Software Updater will automatically check for the latest version of the Operating Software. Click GET UPDATE to download the latest version. Ensure that Automatic Fraction Collector is shown in the Device box, as shown in Figure www.izon.com... -
Page 33: Maintenance Of Afc Instrument
Figure 20: The Izon AFC Update software with the AFC correctly selected in the “Port” dropdown box. If no device is shown in the Device box, close the software and reopen it. The precise name (COM port name) of each AFC will not be consistent across computers. It is common for this to change when the AFC is plugged into different computers and occasionally with the same computer. -
Page 34: Replacing The Nozzle
Figure 23. The tubing should not bow away from the channel as in Figure 24. Be sure to press the tube into the pinching mechanism, located within the channel. www.izon.com... - Page 35 Figure 23: Nozzle tube fully inside the pinch valve. Figure 24: Part of the nozzle tube outside of the pinch valve. Ensure that the tip of the nozzle tube is free from the pinch valve and that the neck of the nozzle tip is protruding from the channel as shown in Figure 25 below.
- Page 36 Figure 27: Nozzle tip hidden behind the pinch valve cover. the pinch valve cover. When reporting AFC issues to support@izon.com, please provide the serial number of the AFC, which can be found on the underside of the AFC as in Figure Figure 28.
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Page 37: Troubleshooting
If there is still no power, try an alternative power source. Power connector is not fully plugged in. Contact Izon support to replace the power supply and/or power lead. Internal connections have loosened during transit. Contact Izon support if the unit flashes when turned on, but does not work. - Page 38 ZERO. Nozzle is bent. Reposition the nozzle to a vertical position or replace. Replacements can be found in the consumables box, or at izon.com. Spillage of liquid Waste tubing to the pump Make sure the waste tubes on the bottom onto the table or is not connected or faulty.
- Page 39 If you have any questions that are not answered on the support portal, please contact our support staff via the online support portal, or directly by email (support@izon.com). FCC Declaration of Conformance: This device complies with part 15 of the FCC Rules. Operation is subject to the following...
- Page 40 FIND OUT MORE. Visit www.izon.com for more information.
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