Related Manuals for Izon AUTOMATIC FRACTION COLLECTOR V2
Summary of Contents for Izon AUTOMATIC FRACTION COLLECTOR V2
- Page 1 A U T O M A T I C F R A C T I O N C O L L E C T O R V 2 ( A F C ) U S E R M A N U A L www.izon.com...
- Page 2 Izon Science Ltd. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorisation of Izon Science Ltd.
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Page 3: Table Of Contents
Choosing Run Parameters ..........................22 Initiating a Run................................27 Completing a Run ..............................29 Resources ................................30 Repair and Servicing of AFC Instrument ......................30 Updating the AFC Firmware ..........................30 Maintenance of AFC Instrument ........................32 Troubleshooting ..............................36 Automatic Fraction Collector V2 User Manual... -
Page 4: Definitions And Writing Conventions
This symbol indicates where special care should be taken Earth (ground) terminal Direct Current (DC) Voltage polarity of the jack Text that appears in pink is used to indicate buttons and SETUP sequences that are used on the AFC touchscreen www.izon.com... - Page 5 The PCV can be Purified Collection Volume (PCV) customised to accommodate different preferences, e.g. to maximise the recovery of extracellular vesicles, or to maximise protein removal. Automatic Fraction Collector V2 User Manual...
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Page 6: Safety And Hazards
< 0.1% sodium azide, which is potentially fatal if swallowed or in contact with skin. When the appropriate qEV column is used on the AFC system, the user is potentially exposed to a dilute solution of sodium azide from the column and/or waste hose outlet. www.izon.com... - Page 7 Do not expose materials that cannot be properly decontaminated to potentially infectious or hazardous material. Upon completion of the tasks involving potentially infectious or hazardous materials, decontaminate the work area with an appropriate disinfectant or cleaning solution (1:10 dilution of household bleach is recommended). Automatic Fraction Collector V2 User Manual...
- Page 8 Before moving or shipping the AFC, decontamination procedures must be performed. Always move or ship the AFC with the supplied packaging materials which will protect the instrument from damage. If appropriate containers cannot be found, contact your local Izon office. Storage The AFC system can be stored under the following conditions: Temperature range: 0 to 60 ˚C...
- Page 9 Alternatively, obsolete equipment and/or consumables may be returned to your local Izon Science representative to be processed appropriately. Contact your local Izon representative for more information.
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Page 10: Introduction To The Automatic Fraction Collector (Afc)
Once the buffer volume has been collected, the instrument will begin collecting a predetermined number of fractions of a given volume as set by the user. The fractions after the buffer volume correspond to the PCV (blue zone Figure. www.izon.com... -
Page 11: Intended Use
Exoid and protein levels by bicinchoninic acid (BCA) assay. 3.2 Intended Use The AFC is used to automate fraction collection of Izon’s qEV columns. The instrument is intended for use in research laboratories by professional personnel for research purposes only. -
Page 12: Instrument Specifications
Carousel Cover Reversible Carousel Carousel Plate qEVoriginal Reservoir 5.0 mL Collection Tube (x15) 2.0 mL Collection Tube (x30) 12 Volt Power Supply Power Lead USB 2.0 Type A to USB 2.0 Micro Cable (male to male) 10 g Calibration Weight www.izon.com... - Page 13 2 mL and 5 mL Axygen tubes (Product codes: MCT-200-C; MCT-500-C, respectively). Figure 3 Figure 4 show the layout of the reversible carousel. Notice that the flush hole does not have a channel for the tube lid. Automatic Fraction Collector V2 User Manual...
- Page 14 A qEV column. Pipettes and tips. Freshly prepared, filtered (0.22 µm) and degassed buffer. Refer to your qEV user manual for more information about buffers. Freshly prepared 0.5 M NaOH solution for column cleaning. 70% ethanol solution for cleaning. www.izon.com...
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Page 15: Assembly And Setup Instructions
Position unit so it can be quickly and easily disconnected from the mains power. Check the local supply meets the AC input requirement given in the specification. Izon instruments are only to be operated with Izon supplied leads and power supplies. Failure to use the correct power supply may result in invalid operation. -
Page 16: Aligning The Carousel
Turn on the instrument using the power switch at the rear. The screen will light up briefly showing the IZON logo and the carousel will rotate to its zero position. Ensure the nozzle set is in place, as shown in Figure Figure 5: AFC nozzle set correctly in place. - Page 17 Improper calibration of the carousel can cause drops from the nozzle to land on the carousel or miss collection tubes. If this occurs, simply recalibrate the carousel. For more information, please refer to Troubleshooting Guide. Automatic Fraction Collector V2 User Manual...
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Page 18: Calibrating The Load Cell
The user can choose whether to leave the tower and halo lights on or off through the Setup Menu. From the home screen, press Settings > Lighting. On the following screen, select or deselect the tower or halo lighting modules. www.izon.com... -
Page 19: Qev Specifications
/ EVs and similarly sized particles have been detected from 0.8 mL on a qEVsingle Legacy column (70 nm Series), however the concentration is extremely low and may cause significant sample dilution if included. Automatic Fraction Collector V2 User Manual... - Page 20 /Loading a lesser volume than the optimal sample loading volume will result in higher sample dilution, while loading a higher volume can overload the column matrix and cause contamination of the purified sample in the PCV. b /Buffer volumes stated are exclusive of an additional 0.284 mL from the volume of buffer inside the nozzle set. www.izon.com...
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Page 21: Operating Instructions
Insert the column into the column mount, then remove the lower cap and carefully dock the column. Ensure that the Izon logo on the column is facing away from the AFC tower as shown in Figure Automatic Fraction Collector V2 User Manual... -
Page 22: Choosing Run Parameters
The maximum number of fractions that can be collected per run is 30. If you have chosen a fraction count that is larger than the carousel’s capacity, the run will pause after the last fraction on the carousel has been filled, and an alarm will sound to indicate it is time to replace the tubes on the carousel. www.izon.com... - Page 23 5.0 mL Min Fraction Volume 0.17 mL 0.4 mL 0.7 mL 5.0 mL ++ 0.17 mL ++ 0.5 mL ++ 0.7 mL Fraction Volume Increments + 0.05 mL + 0.1 mL + 0.1 mL Automatic Fraction Collector V2 User Manual...
- Page 24 The AFC will display “invalid column” when it has been used a total of 10 times, as shown in Figure Figure 12: A warning indicator shows how many uses a column has left after the recommended uses have been exceeded. Figure 13: The home screen displaying when a column is invalid. www.izon.com...
- Page 25 Figure 16: The Volume screen allows you to adjust the volume to be collected per fraction. Press -/+ to adjust in small increments or --/++ to adjust in large increments. Press Set to save your parameters and return to the home screen. Automatic Fraction Collector V2 User Manual...
- Page 26 Ensure that you have loaded enough tubes for your collection protocol. Gently place the carousel onto the carousel plate. Ensure that the carousel alignment hole engages with the raised carousel alignment pin on the carousel plate as shown in Figure www.izon.com...
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Page 27: Initiating A Run
If you choose to flush the column, wait for all of the buffer to pass through the column, then press to continue. If you choose to prime the column, wait for buffer to start dripping from the nozzle, then press to continue. Automatic Fraction Collector V2 User Manual... - Page 28 www.izon.com...
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Page 29: Completing A Run
Before turning off the AFC, rinse the AFC nozzle using DI water with the valve open. This will prevent salt crystallising in the tip of the nozzle while it is not in use. Switch off the AFC at the rear of the instrument. Automatic Fraction Collector V2 User Manual... -
Page 30: Resources
RESOURCES 8.1 Repair and Servicing of AFC Instrument All replacement parts must be obtained via Izon Science Ltd. Contact your regional Izon representative for replacement parts. We recommend that you save the box and packaging materials in case the instrument needs to be returned for servicing. - Page 31 Connect the computer to the rear of the AFC instrument using the provided USB cable. Open the AFC Updater. The AFC Updater will automatically check for the latest version of the Operating Firmware. Click Get Update to download the latest version. Automatic Fraction Collector V2 User Manual...
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Page 32: Maintenance Of Afc Instrument
Ensure that Automatic Fraction Collector is shown in the Port box, as shown in Figure 22. Figure 22: The Izon AFC Updater with the AFC correctly selected in the “Port” dropdown box. If no device is shown in the Port box, close the software and reopen it. - Page 33 Open the settings menu and navigate to the fluid tab, then toggle the valve open. Remove the pinch valve cover by firmly grasping the pinch valve cover and gently pulling away from the AFC tower. Automatic Fraction Collector V2 User Manual...
- Page 34 If the tip is hidden behind the pinch valve cover, then the tube has been inserted too high in the pinch valve. Simply grasp the nozzle tip and pull gently down (distally) to move the tube further down in the pinch valve channel. www.izon.com...
- Page 35 Figure 28: Nozzle tip fully emerging through the hole in Figure 29: Nozzle tip hidden behind the pinch valve cover. the pinch valve cover. Automatic Fraction Collector V2 User Manual...
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Page 36: Troubleshooting
Power connector is not fully work plugged in. Internal connections have Contact Izon support. loosened during transit. Please provide the serial number (underneath the unit). Leakage from the column Column is not connected... - Page 37 If the pump is silent during the flushing steps, Pump is faulty. contact Izon support. Waste tube not in waste Ensure that the free end of the tubing is in a container. waste container. Automatic Fraction Collector V2 User Manual...
- Page 38 Always remove the top cap first, while the qEVsingle, air trapped below lower cap is still on. the white frits or in the nozzle Contact Izon support. tube may be impeding the flow. AFC flush hole will not If no sound can be heard Contact Izon support.
- Page 39 When reporting AFC issues to Izon support, please provide the serial number of the AFC, which can be found on the underside of the AFC as in Figure 30.
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