Method Of Pipetting - Labnet BioPette PLUS Quick Start Manual

MULTICHANNEL
The pipette design enables the user to perform the recali-
bration process according to the rules presented in sec-
tion 5.
2 - SETTING THE VOLUME
The setting of the volume of the aspirated liguid can be
done either by the pipetting pushbutton knob (Fig. 1A2)
or by the adjustment knob (Fig. 1B). The volume adjust-
ment can be performed when the locking ring is set in
the lower position (Fig. 5A). When the desired volume is
selected, the locking ring should be set in the upper
position (Fig. 5B).
The volume shown by the counter is represented by
three digits, which should be read from top to bottom.
Typical meter readings are shown in the following table:
Counter
Model
readings
0
BPPE-10
3
BPPT-10
5
0
BPPE-50
6
BPPT-50
5
0
BPPE-200
8
BPPT-200
5
2
BPPE-300
5
BPPT-300
0
To attain the maximum accuracy, set volume must be
approached from a higher value by diminishing counter
readings.
• If the desired volume is lower than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the adjustment knob (Fig. 1B)
to the direction diminishing counter readings to the
required volume. Before achieving the required volume
slowly rotate the knob and observe carefully diminish-
ing reading to avoid accidentally passing the setting
value.
• If the desired volume is higher than set volume shown by
the counter, the operator should turn the pipetting push-
3
Set Basic
volume degree
3.5 μl 0.02 μl
6.5 μl 0.10 μl
85.0 μl 0.20 μl
250.0 μl 1.0 μl
button (Fig. 1A2) or the adjustment knob (Fig. 1B) increas-
ing the value until the lower figure wheel comes 1/3 of a
turn beyond the required setting and then slowly backward
until the setting reaches the desired volume. Make sure not
to pass the setting value.
If the knob is accidentally turned too far, the process
must be repeated. The desired volume must always be
set from the higher value in the order of decreasing value.
Following volume adjustment, set the locking ring into the
upper position, thus locking the knob and preventing
accidental volume change.

3 - METHOD OF PIPETTING

Fit the tips onto cones of the multichannel module. In
order to put tips on the multichannel
pipette vertically and press it against the tips in the rack
box, until the cones retreat about 1.5 mm into the multi-
channel module. The suspension system of the
ensures even and tight sealing of tips. The pipette does
not need to be rolled back and forth to seal the tips
tightly. The liquid aspirated into the tips should not flow
out by gravity from properly fixed tips. While holding the
pipette in a vertical position, tips should be immersed in
the liquid to a depth of 2-4 mm, and flushed once by
drawing a dose of liquid and dispensing it out with slow
and steady movement. Next, still holding the pipette ver-
tically, the operator should press the pipetting button
until the first resistance point is felt, and immerse the tips
in the liquid to the depth of 2-4 mm, (Fig. 3B). Releasing
the pipetting button with a slow and uniform movement
during 2-3 seconds, the liquid should be drawn into the
tips, (Fig. 3B) which should then be lifted above the liq-
uid's surface. The pipette should next be positioned at an
angle of 10-45 degrees in relation to inner walls of desti-
nation vessels and tips should be emptied by pressing
the pipetting button slowly until the first resistance point is
felt, (Fig. 3C). After waiting second, the pipetting button
should be pressed to the second resistance point in
order to expell remaining liquid, (Fig. 3D). Then tips
should be lifted out from the vessels while maintaining
contact between the ends of the tips and the inner walls
of the vessel until the pipetting button has been relased,
(Fig. 3E). Finally, pressing the ejector button, the opera-
tor should separate the tips from multichannel module's
cones, (Fig. 3F).
cones hold the
cones
4