Brooks CoolBox 2XT Instructions For Use

CoolBox
Overview of CoolBox
CoolBox 2XT is designed for maintaining sample temperatures below 4°C on the bench top with-
out the use of wet ice or electricity. Patent-pending dual-phase conductive XT Cooling Core or XT
Freezing Core provides the cooling source when a thermo-conductive CoolRack
or plate module is placed on top. The core and sample module in combination ensure uniform
well-to-well temperature throughout the cooling period regardless of sample position. XT Cooling
Core can maintain sample temperature from 0.5 to 4°C for over 16 hours; XT Freezing Core can
maintain frozen samples for 10 hours; using dry ice in the base provides an ultra-cold temperature
(-78°C) for snap-freezing samples in tubes or plates. For a list of CoolRack and CoolSink modules
that are compatible with the CoolBox 2XT, see back page.
Working
Temperature Range
0.5° to 4 o C
-20° to 0 o C
-78 C
o
*All tests were performed using a CoolRack
tubes filled with 1.5 mL water. Actual performance may vary depending upon CoolRack
ployed, sample load, initial sample temperature, ambient temperature, air currents, and other conditions.
Quick Start
• Remove two XT Cooling Cores from -20°C
freezer and place on bench.
• When temperature strip on XT Cooling
Core registers 1°C, place CoolRack
CoolSink sample modules in the Cool-
®
Box XT base and place collar on top.
™
• Load samples in CoolRack or CoolSink
modules.
IMPORTANT: To ensure your samples do not freeze and to get maximum cooling duration, please
refer to the detailed instructions that follow.
Assembly of CoolBox
The CoolBox 2XT system consists of a closed-cell, cross-linked polyethylene foam base, collar, lid
and a reusable XT Cooling Core. A CoolBox 2XT also includes a CoolRack
plate module. When placed on top of the XT Cooling or Freezing Core, the modules and samples
rapidly equilibrate to the temperature of the XT Core. Samples can be placed in the CoolRack
or CoolSink module either before or after the module is placed in CoolBox XT. When used with
CoolSink plate modules the magnetized collar is not required and can be removed for easy access
when working; however, to obtain the maximum cooling duration, we recommend using the collar
whenever possible.
2XT
TM
2XT System
™
CoolBox XT
Cooling Source
XT Cooling Core
XT Freezing Core
200 ml Dry Ice
or
®
2XT
™
Temperature Duration
Open Lid
Over 10 hours
Over 5 hours
Over 4 hours
XT M24 loaded with 24 TruCool
®
Caution
• Lift CoolBox 2XT from the bottom using
the handholds on the sides.
• Always use two hands when carrying or
lifting CoolBox 2XT.
• Avoid touching the top metal surface of
the XT Cooling Core and XT Freezing Core
when removing from freezer.
Instructions for Use
or CoolSink
®
Temperature Duration
*
Closed Lid
Over 16 hours
Over 8 hours
Over 5 hours
2.0 mL microcentrifuge
®
module em-
®
or CoolSink
® ®
tube
®
*
tube or