Brooks CoolCell SV10 Instructions For Use

CoolCell
Controlled-rate cell freezing container
Quick Start
• The six chambers and 10ml serum vials should be dry to avoid vial sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials, each containing 5ml of cell suspension, in each well. For reproducible freezing
profiles, each well should contain a filled vial; "blank" vials containing 5ml of freezing medium should be
placed in empty chambers if freezing less than six vials of cell suspension.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell SV10 container.
• Place CoolCell SV10 container into a -8o
of free space clearance around the CoolCell SV10 module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell SV10 container from the freezer and gently remove the lid using a twisting and rocking
motion.
• Vial tops will be exposed once lid is removed and vials should be quickly placed onto the dry ice.
Special Notes:
• Always use dry ice to transfer frozen vials containing cells to permanent storage to avoid temperature
rise and cell damage. Vial contents can rise from -80
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell SV10 to room temperature
The CoolCell SV10 container is ready to freeze again as soon as the core (black ring) is at room temperature.
To rapidly recycle CoolCell SV10 container to room temperature, remove the center solid core ring by invert-
ing and tapping on a surface. The CoolCell SV10 body and lid will return to room temperature in 10 to 15
minutes. Check that all chambers are dry. Dry the core ring before re-inserting into the central chamber.
About CoolCell SV10
The CoolCell SV10 container, in combination with a -80
rate of -1 per minute that is ideal for cryo-preservation of most cultured cell lines. The CoolCell SV10 design
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uses a combination of insulation foam, radial symmetry, and a heat transfer core to regulate heat loss rather
than using a large thermal mass (alcohol-based freezing container).
SV10
®
freezer or dry ice locker. Ensure that there is at least one inch
o
C
to over -50 in less than one minute if exposed to
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C C
freezer or dry ice locker, will provide the freezing
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Instructions for Use