Gas Sample Port; Chamber Lid Plug - K-Systems G210 InviCell User Manual

Maintenance

Gas Sample Port

The Gas Sample Port is located behind the Preparation Chamber. The Gas Sample Port is connected
directly to the gas-mixing chamber.
1. Connect a short length of tubing to the gas analyzer input.
2. Connect the enclosed coupling to the other end of the tubing.
3. Remove the protection cap from the sample port.
4. Connect the coupling to the incubator sample port.
5. Connect a short length of tubing to the gas analyzer output.
6.
Connect the other end into the lid plug of chamber 8, using a 25 - gauge needle. This re-
circulates gas back into the incubator.
To obtain a faster stable reading from the sample port, or chamber lid plug, purge the gas
anaylzer of gases by
• Running the pump (no more than) 30 seconds
• Stopping the pump for 30 - 40 seconds to allow the gas to stabilize
7. Collect the gas sample.
8. Run the pump for (no more than) 50 seconds.
9. Stop the pump.
10. Record the gas concentration reading.
11.
Disconnect the coupling from the sample port.
12.
Replace the protection cap.
If calibration is needed, adjust the value on the calibration, wait for the gas to stabilize and re-test (no
need to purge gas analyzer if already connected).
Gas flow to the Preparation Chamber should be switched back on (see page 26 Changing the
Preparation Settings).
12

Chamber Lid Plug

A silicone plug is placed in the lid of each chamber. For collecting a gas sample, penetrate the lid plug
with a needle attached to tubing and connect to the gas analyzer outlet. Do not use needles larger
than 0.5 mm x 25 mm. The angle for penetration should be vertical (+/-10 degrees) and no horizontal
movements should be made. Each plug should not be penetrated more than 5 times after which it
should be replaced.
42
1 2 3