Validation Check; Gas Calibration - K-Systems G210 InviCell User Manual

7.
Complete validation check.

Validation Check

Perform the following validation gas and temperature checks after all cleaning, disinfecting and
sterilizing or at least every two weeks to ensure the G210 InviCell is operating correctly.

Gas Calibration

It is VERY important that the G210 is not emptied of gas during the calibration procedure. To do so
will cause very unstable gas levels and gas flow, and will result in a considerable time before the gas
concentration is recovered and becomes reliable and stable again.
To ensure the G210 is not emptied of gas during the gas sampling please follow the below instructions.
There are two methods for collecting gas samples from the incubator:
1. Via the Gas Sample Port (located behind the Preparation Chamber)
2.
Via the silicone plug on each of the chamber lids
DO NOT collect more than 0.1l of gas from either mixing chamber or chambers.
Always check the gas concentration in the setpoint menu during sampling. If the gas concentration
differs more than 0.1% from the setpoint, allow the G210 to stabilize to the gas concentration set
point before taking the next gas sample.
Please note: To ensure accurate and reliable gas measurements, please use a high quality calibrated gas
analyzer. The calibration procedure described here assumes the use of the G100 gas analyzer (Order
Code: 11103) which recirculates gas into the gas flow during sampling. If not using a recirculating gas
analyzer, allow the incubator to stabilize for 3 minutes after each sample collection. Ensure the gas
analyzer is prepared as per the gas analyzer user manual.
Gas flow to the Preparation Chamber should be disconnected during gas calibration (see page 26
Changing the Preparation Chamber Settings).
Maintenance
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