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Operation - National DC4-156-S Instructions For Use Manual

Compound biological microscope with digital camera

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10. SAFETY RACK STOP: When properly adjusted, controls maximum upward travel of stage. Prevents higher
power objectives from breaking specimen slides, prevents damage to objective lenses. This stop has been
pre-adjusted at the factory.
11. FOCUSING KNOBS: Coarse focusing knobs (larger knobs) located on each side of arm, raise or lower
stage to bring specimen image into focus. Fine focus knobs (smaller knobs located just below coarse
focusing knobs) permit more precise image adjustment.
12. ILLUMINATION: Built-in substage electric illuminator provides constant, reliable pre-focused illumination.
13. RHEOSTAT INTENSITY CONTROL: Controls illumination intensity. Always turn control to lowest intensity
before turning microscope power on or off. This extends bulb life.
14. 12VDC POWER CORD/CONVERTER: Accepts 100v-240v 50H/60H input and supplies 12VDC power to
the microscope. Note that this converter accommodates either 120v or 240v and 50H or 60H power supply,
eliminating the need for any additional transformer.
15. ON/OFF SWITCH:
16. USB 2.0 CABLE: Connects microscope & camera to computer.

OPERATION

Your microscope is fully functional as a standard microscope. The following instructions apply only to operation
of the microscope. Refer to the software instructions for installation of the software and operation of the camera.
Some steps for microscope operations are altered slightly in the software documentation, in order to utilize some
of the unique features provided by the digital camera and software.
1. Illumination.
A. Adjust intensity control on side of base to the minimum position. This should be done prior to
each time light is turned on or off, in order to extend bulb life.
B. Plug one end of power cord into 12VDC switching power converter and other end into power outlet. Plug
12VDC converter into power jack on base of microscope. Flip power switch on microscope base "on".
Note that camera LED indicator will not light until USB 2.0 cable is connected to computer, and camera
turned on by software commands.
2. Focusing the microscope.
A. Position the 4x objective lens into the optical path, making sure that lens is properly indexed in its click-
stop position.
B. Swing moveable finger on slide holder outward. Place specimen slide (cover slip up) on top of stage
surface against fixed side of slide holder. Slowly release moveable finger until it makes contact with
specimen slide.
C. Rotate coarse focusing controls until specimen comes into focus.
D. Adjust fine focus controls until specimen is in sharp focus.
E. Adjusting the aperture (opening) of iris diaphragm.
Iris diaphragm should not be used to control the brightness of illumination. Iris diaphragms are designed
to help achieve high resolution of specimen and provide contrast in the image. Smaller apertures will
deliver higher contrast to image.
Experimentation is the best method of determining the correct opening of diaphragm. Some suggested
openings for iris diaphragm are:
This turns microscope lamp on
However, closing aperture too much will reduce resolution.
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