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13. RHEOSTAT INTENSITY CONTROL: Controls illumination intensity. Always turn control to lowest intensity before
turning microscope power on or off. This extends bulb life.
14. POWER CORD: Attached US 110V 60Hz, three prong UL approved power cord.
15. ON/OFF SWITCH:
16. USB 2.0 CABLE: Connects microscope & camera to computer.
OPERATION
Your microscope is fully functional as a standard microscope. The following instructions apply only to operation of the
microscope. Refer to the software instructions for installation of the software and operation of the camera. Some steps
for microscope operations are altered slightly in the software documentation, in order to utilize some of the unique features
provided by the digital camera and software.
1. Illumination.
A. Adjust intensity control on side of base to the minimum position. This should be done prior to each time light
is turned on or off, in order to extend bulb life.
B. Plug one end of power cord into power outlet. Flip power switch on microscope base "on". Note that camera LED
indicator will not light until USB 2.0 cable is connected to computer, and camera turned on by software commands.
2. Focusing the microscope.
A. Position the 4x objective lens into the optical path, making sure that lens is properly indexed in its click-stop
position.
B. Swing moveable finger on slide holder outward. Place specimen slide (cover slip up) on top of stage surface
against fixed side of slide holder. Slowly release moveable finger until it makes contact with specimen slide.
C. Rotate coarse focusing controls until specimen comes into focus.
D. Adjust fine focus controls until specimen is in sharp focus.
E. Adjusting the aperture (opening) of iris diaphragm.
Iris diaphragm should not be used to control the brightness of illumination. Iris diaphragms are designed to help
achieve high resolution of specimen and provide contrast in the image. Smaller apertures will deliver higher
contrast to image. However, closing aperture too much will reduce resolution. Experimentation is the best
method of determining the correct opening of diaphragm. Some suggested openings for iris diaphragm are:
OBJECTIVE
a) Placing the frosted filter into the optical path will absorb and diffuse some of the light from the illuminator base,
limiting the light output.
F. Changing magnification.
a) Rotate revolving nosepiece to position 10x objective into optical path.
b) This microscope has been parfocalized, which allows changes from one objective to another while requiring
only a slight adjustment of the fine focus controls.
This turns microscope lamp on
4x
10x
40x
100x
DIAPHRAGM OPENING
From fully closed to 1/8 open
1/8 to 1/4 open
1/4 to 1/2 open
1/2 to 3/4 open
4
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