BIO RAD PowerPac HV Series Instruction Manual
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BIO RAD PowerPac HV Series Instruction Manual

BIO RAD PowerPac HV Series Instruction Manual

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PowerPac
HV
Power Supply
Instruction Manual
Catalog Numbers
164-5056
and
164-5059

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Summary of Contents for BIO RAD PowerPac HV Series

  • Page 1 ™ PowerPac Power Supply Instruction Manual Catalog Numbers 164-5056 164-5059...
  • Page 3 Technical Support For Technical Support contact your local Bio-Rad office. In the US call 1-800-424-6723. Please have the following information available. Model number: Located on the sticker on the bottom of the unit. Serial number: Located on the sticker on the bottom of the unit. Firmware version: The PowerPac HV displays the software version momentarily after switching the power ON.
  • Page 5 Safety Read this page before proceeding. Bio-Rad PowerPac power supplies are designed and certified to meet EN 61010* safety standards. Certified products are safe to use when operated in accordance with the instruction manual. This safety certification does not extend to electrophoresis cells or accessories that are not EN 61010 certified, even when connected to this power supply.

  • Page 7: Table Of Contents

    Table of Contents Page Section 1 Introduction ................1 Overview....................1 Features ....................2 Unpacking....................3 Section 2 Control Panel ................4 Section 3 Product Setup................5 Power Supply Connections ..............5 Preferences Setup ..................6 Explanation of Safety Features and Setup Options .........7 3.3.1 Pfd: Power Failure Detection .............7 3.3.2 RRCd: Rapid Resistance Change Detection......8 3.3.3...
  • Page 8 List of Figures Figure 1. Front View....................1 Figure 2. Rear View ....................1 Figure 3. Front Panel with Legs ................2 Figure 4. Front Panel ....................4 Figure 5. Power Leads Connected Correctly ............5 Figure 6. Power Leads Connected Incorrectly ............5 Figure 7. Menu Overview for Manual Operation ............11 Figure 8.

  • Page 9: Introduction

    PowerPac HV – Instruction Manual Section 1 Introduction 1.1 Overview The PowerPac HV power supply is designed to provide constant voltage, current, or power for a wide range of electrophoresis applications, including IEF and SDS PAGE. It also provides temperature control for DNA sequencing with the Sequi-gen cell. The PowerPac HV operates at the constant value specified by the user.

  • Page 10: Features

    PowerPac HV – Instruction Manual 1.2 Features The PowerPac HV has the following features: • Constant voltage, voltage ramping, constant current or constant power operation with automatic crossover and temperature control mode • Backlit LCD screen • Continuous display of all parameters •...

  • Page 11: Unpacking

    PowerPac HV – Instruction Manual 1.3 Unpacking When the power supply is received, carefully inspect the container for any damage which may have occurred in shipping. Severe damage to the container may indicate damage to the power supply itself. If you suspect damage to the unit, immediately file a claim with the carrier in accordance with their instructions before contacting Bio-Rad Laboratories.

  • Page 12: Control Panel

    PowerPac HV – Instruction Manual Section 2 Control Panel ALPHANUMERIC RUN/PAUSE KEY KEYPAD pqrs wxyz STOP/HOME KEY CLEAR ENTRY KEY EDIT SETUP KEY IR PORT FUNCTION KEYS EDIT KEY CURSOR KEYS Fig. 4. Front Panel. Description Run/Pause Starts or pauses a run. When paused, the run parameters for the current or subsequent step(s) may be edited.

  • Page 13: Product Setup

    PowerPac HV – Instruction Manual Section 3 Product Setup 3.1 Power Supply Connections This section describes how the PowerPac HV power supply is connected to an electrophoresis cell or cells. Step Procedure Description Connect the Connect one end of the included power cord to the power cord rear of the instrument.

  • Page 14: Preferences Setup

    PowerPac HV – Instruction Manual Step Procedure Description Turn power on Use the switch located on the right side of the power supply to power on. Initially, the unit will display the firmware version and serial number for a few seconds, then go to the Home screen.

  • Page 15: Explanation Of Safety Features And Setup Options

    PowerPac HV – Instruction Manual 3.3 Explanation of Safety Features and Setup Options 3.3.1 Pfd: Power Failure Detection Preference Options Description OFF: Turns power failure detection OFF. Power Failure Detection (Default) In this mode, the run is terminated if a NEXT RUN power failure occurs.

  • Page 16: Rrcd: Rapid Resistance Change Detection

    PowerPac HV – Instruction Manual 3.3.2 RRCD: Rapid Resistance Change Detection Preference Options Description RRCd ON: Detection of a sudden resistance Rapid Resistance Change (Default) change greater than 20% will cause an detection alarm to sound, the run to pause, and error message code 09 to be displayed.
  • Page 17 PowerPac HV – Instruction Manual KEY CHIRP Activates the audible signal for keystroke. (Default) IRDA CONTRAST * KEY CHIRP CHANGE EXIT RESET DATE MM/DD/YY This is the only format available. Press the change soft key then enter the ETUP correct date using the alphanumeric KEY CHIRP keypad.

  • Page 18: Operation

    PowerPac HV – Instruction Manual Section 4 Operation The PowerPac HV has four operation modes: 1. Manual: Single-step run, not stored in memory. (see Section 4.1) 2. Basic Methods: Nine (9) methods with up to nine steps can be stored. (see Section 4.2) 3.

  • Page 19: Manual Mode

    PowerPac HV – Instruction Manual 4.1 Manual Mode Manual mode allows the user to program and start a single-step method with a minimum number of keystrokes. This single-step method is not stored in permanent memory. This section describes how to program and perform a run in Manual mode. SELECT MODE METHODS MANUAL...

  • Page 20: Create, Edit And Run In Manual Mode

    PowerPac HV – Instruction Manual 4.1.1 Create, Edit and Run in Manual Mode Step Procedure Description Set up the power Refer to Section 3 for instrument setup. supply Select the mode From the Home screen, press the MANUAL soft key to perform a single-step run in Manual mode. ELECT MODE When in doubt, press EDIT to toggle between screens without losing entered values.
  • Page 21 PowerPac HV – Instruction Manual Step Procedure Description Start the run Press to start a run. A run may be started from screen of the EDIT loop as long as a valid constant parameter value has been entered. Monitor the run The run screen displays the elapsed time versus the programmed time (timed runs), elapsed time (untimed run), voltage, current and power values.

  • Page 22: Basic Methods Mode

    PowerPac HV – Instruction Manual 4.2 Basic Methods Mode Basic Methods Mode is used to create, run and store basic methods. It has the same programming options (edit loop) as the Manual mode. The method list stores up to nine methods, with up to nine steps each.

  • Page 23: Create, Edit And Run A Basic Method

    PowerPac HV – Instruction Manual 4.2.2 Create, Edit and Run a Basic Method This section describes how to create, open, modify and run Basic Methods. Step Procedure Description Set up the power supply Refer to Section 3 for instrument setup. Select the operation At the Home Screen: mode...
  • Page 24 PowerPac HV – Instruction Manual Step Procedure Description Method steps Press EDIT to enter the edit loop. New method • Press the up and down arrows to select an existing step or select the (EMPTY) step to add a METHOD: NAME new step (EMPTY) •...
  • Page 25 PowerPac HV – Instruction Manual Step Procedure Description Select the time mode Use the soft keys to select a time mode and the alphanumeric keypad to enter the run time. HRS: Run time is in units of hours. S1/1 EDIT TIME 500 mA VHOURS: Run time is in units of volt-hours.

  • Page 26: Ief Methods Mode

    PowerPac HV – Instruction Manual 4.3 IEF Methods Mode The IEF Method mode is used to create, run and store Isoelectric focusing (IEF) methods. The method list stores up to nine methods, with up to nine steps each. Special programming options such as voltage ramping and run delay in the first step, allow better control during IEF separations.

  • Page 27: Create, Edit And Run An Ief Method

    PowerPac HV – Instruction Manual 4.3.1 Create, Edit, and Run an IEF Method This section describes how to create and edit methods. Step Procedure Description Set up the power supply Refer to Section 3 for instrument setup. Select the operation At the Home screen, press the IEF METHODS soft mode key to display the IEF Method List screen.
  • Page 28 PowerPac HV – Instruction Manual Step Procedure Description Method name Name a method. • Press the up arrow key to select the method name field. • Use the CE button to clear the name field and the alphanumeric keypad to enter a method name. Refer to Section 2 for help using the keypad.
  • Page 29 PowerPac HV – Instruction Manual Step Procedure Description Enter parameter limits Current or power limits must be set according to application instructions to prevent overheating of the IEF device . Use the soft keys to select a limit parameter and the keypad to enter its value.

  • Page 30: Temperature Mode

    PowerPac HV – Instruction Manual Step Procedure Description The Run Completed or Run Terminated screen is displayed at the end of a run. Press RERUN to repeat the run or EXIT to display the method step list. 4.4 Temperature Mode The Temperature operation mode is used to run an electrophoresis apparatus at constant temperature.

  • Page 31: Create, Edit And Run In Temperature Method

    PowerPac HV – Instruction Manual 4.4.1 Create, Edit and Run in Temperature Method Step Procedure Description Set up the power supply Refer to Section 3 for instrument setup. Connect temperature The Temperature mode may be accessed from any probe to the back of screen, except for the run screen, by connecting the the PowerPac HV probe.
  • Page 32 PowerPac HV – Instruction Manual Step Procedure Description Select the time mode Use the soft keys to select a time mode and the alphanumeric keypad to enter the run time. DIT TIM HRS: Run time is in units of hours. 55 W 5000 VHOURS: Run time is in units of volt-hours.

  • Page 33: Edit A Paused Run

    PowerPac HV – Instruction Manual 4.5 Edit a Paused Run The PowerPac HV permits editing the run parameters during a pause in all operation methods modes: Manual, Basic, IEF Methods, and Temperature Mode. The edit capability during Pause is limited to the current and subsequent steps, and follows the same edit sequence used when programming the original run.

  • Page 34: Editing A Paused Run

    PowerPac HV – Instruction Manual 4.5.1 Editing a Paused Run Step Procedure Description Pause the run Press the PAUSE soft key or to pause a run that is in progress. The S#/# indicates this run is performing Step 1 of 2 steps. Edit the method The pause window displays the current run parameters as well as the date and time that the run...

  • Page 35: Data Transfer Using The Infrared (Ir) Port

    PowerPac HV – Instruction Manual Section 5 Data Transfer Using the Infrared (IR) Port and Data Transfer Software 5.1 Overview The PowerPac HV is capable of transmitting methods and data through an infrared (IR) port located on the front of the power supply (Figure 13). This optional PowerPac Data Transfer Software (DTS)(catalog #164-5067) includes the PowerPac Remote application for a PDA.

  • Page 36: System Requirements

    PowerPac HV – Instruction Manual 5.2 System Requirements Minimum PC specifications • Windows 2000 or Windows XP Operating System • 400 MHz processor • 256 MB RAM minimum, 512 MB recommended • 1,024 x 768 screen resolution, with true-color mode (24 or 32 bits) •...

  • Page 37: Maintenance And Troubleshooting

    PowerPac HV – Instruction Manual Section 6 Maintenance and Troubleshooting 6.1 Maintenance The PowerPac HV requires little maintenance to ensure reliable operation. To clean the case, first unplug the power supply. Use a soft, damp cloth to wipe the outer case. Keep the PowerPac HV away from liquids.

  • Page 38: Replacement Parts

    PowerPac HV – Instruction Manual 6.1.2 Replacement Parts Catalog Number Description 900-8933 Replacement Fuse, 8 A, 250 V, 5 x 20 mm, type T 165-9005 Power Cable 165-5058 Temperature Probe 6.2 Troubleshooting Problem Possible Cause Solution No display/lights/fan 1. No AC power. 1.

  • Page 39: Error Messages

    PowerPac HV – Instruction Manual 6.3 Error Messages Error Message Possible Cause Solution ERROR STOP: CODE 01 1. Electrophoresis cell 1. Make sure all electrical NOLOAD DETECTED not connected to the connections are making good CORRECT AND CONTINUE power supply or contact and the cables RUN PAUSED buffer levels too low.
  • Page 40 PowerPac HV – Instruction Manual Error Message Possible Cause Solution ERROR STOP: CODE 09 1. Loose output 1. Verify all electrical RAPID CHANGE IN R connections leading connections. CORRECT AND CONTINUE to intermittent RUN PAUSED connection to the loads. 2. Cells added or 2.
  • Page 41 PowerPac HV – Instruction Manual Error Message Possible Cause Solution ERROR STOP: CODE 18 The set temperature cannot TEMP REG ERROR be reached because: CORRECT AND CONTINUE 1. The temperature probe is 1. Make sure the temperature RUN PAUSED no longer connected to probe is securely fastened to the electrophoresis cell the electrophoresis cell.

  • Page 42: Appendix A Technical Specifications

    PowerPac HV – Instruction Manual Appendix A Technical Specifications Output (programmable) Voltage 20–5,000 V Current 0.05–500 mA Power (maximum) 1–400 W Output regulation Constant voltage, constant current, constant (with automatic crossover) power, or constant temperature Timer control 1 min to 99 hr, 59 min Volt-hour control Yes, 99,999 V-hr Pause/resume function...

  • Page 43: Appendix B Warranty And Ordering Information

    Appendix B Warranty and Ordering Information Warranty The PowerPac HV power supply is covered by a standard Bio-Rad Laboratories warranty. Contact your local Bio-Rad representative for details of the warranty. If any defects should occur during this warranty period, Bio-Rad Laboratories will replace the defective parts without charge.
  • Page 48 Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699 Life Science Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 Group France 01 47 95 69 65 Germany 089 31 884 0...

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