Model 200/2.0 Power Supply Instruction Manual Catalog Numbers 165-4761 and 165-4762 For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)
Table of Contents Page Section 1 General Information..................1 General Information ....................1 Safety ..........................1 Specifications ......................2 Diagram of Front Panel....................3 Description of Membrane Control Pad..............3 Section 2 Operation ......................4 Standard Mode Operation ..................4 Time Mode Operation ....................5 Checking and Changing Operating Parameters............5 Section 3 Blotting Applications ...................6 Required Steps for Proper Blotting Results...............6...
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Note To insure best performance from the Model 200/2.0 Power Supply, become fully acquainted with these operating instructions before use. Model Catalog No. Date of Delivery Warranty Period Serial No. Invoice No. Purchase Order No. Warranty Bio-Rad Laboratories warrants the Model 200/2.0 Power Supply against defects in materials and workmanship for 1 year.
2. Always connect the supply to a separate 3-prong, grounded AC outlet. Handle only one lead wire at a time when connecting a load to the power supply. 3. Do not operate the Model 200/2.0 power supply in extreme humidity (≥ 95%), or where condensation can short the electrical elements.
1.3 Specifications Output voltage 5-200 volts DC, continuously adjustable Output current 0.01-2.0 A, continuously adjustable Output terminals Floating dual output jacks in parallel Set limits Current: 0.01-2.0 A, 0.01 A steps, fully adjustable Power: 200 watts maximum output Automatic crossover upon reaching limit. Timer control 0.1-99.9 hours, 0.1 H steps, continuously adjustable Set point resolution...
1.4 Diagram of Front Panel Membrane Control Pad 1.5 Description of Membrane Control Pad 1. LED display Displays the current limit, voltage, and timer parameters while they are being set. Displays the operating parameters of current, current limit, voltage, time, load resistance, power output, and volt-hours during a run.
Section 2 Operation 2.1 Standard Mode Operation General Application: The standard mode is used in blotting applications and in nucleic acid electrophoresis experiments where timing the run is not important. In this mode, the supply is programmed to run at constant voltage within set current limits. Set the Current Limit 1.
(equivalent to a night light). 2.2 Time Mode Operation General Application: The Model 200/2.0 power supply can operate at constant voltage or constant current for a set time. The power supply automatically shuts off and the alarm sounds when the set time has elapsed.
3. Press LIMIT to view the set current limit. The red indicator light above the button should be on, and the set limit will be shown on the LED display. To adjust the current limit, follow the procedure in Section 2.1. 4.
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8. Sponge pads can not dissipate heat as effectively as Scoth Brite ® gel pads. For high power, high intensity applications, it is advisable to use gel pads in place of sponge pads to minimize localized heating effects. 9. Do not increase voltage settings beyond those indicated in Table 1 for overnight operation. If overnight transfers at low voltages are ineffective for your application and you must increase the voltage, you must also decrease the transfer time and/or provide external cooling.
Conductivity of buffer is too high. 5. Fuse blows frequently. 5a. Abnormality inside unit. 5a. Contact Bio-Rad or technical representative. 6. LED displays OFF, 6a. Timer has counted down 6a. Press one of the buttons alarm is sounding.
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China, Bio-Rad Laboratories, 14, Zhi Chun Road, Hai Dian District, Beijing 100088 • Phone (01) 2051873 • Fax (01) 2051876 Hercules, California 94547 France, Bio-Rad S.A., 94/96 rue Victor Hugo, B.P. 220, 94 203 Ivry Sur Seine Cedex • Phone (1) 49 60 68 34 • Fax (1) 46 71 24 67 Telephone (510) 741-1000 Germany, Bio-Rad Laboratories GmbH, Heidemannstraße 164, D-80939 München/Postfach 450133, D-80901 München •...
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