BIO RAD PowerPac 1000 Instruction Manual
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BIO RAD PowerPac 1000 Instruction Manual

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BIO RAD PowerPac 1000 Instruction Manual

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Summary of Contents for BIO RAD PowerPac 1000

  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

  • Page 2: Power Pac

    – + C LR – + POWER PAC 1000 POWER PAC 3000 INSTRUCTION MANUAL Catalog Numbers PowerPac 1000 165-5054 165-5055 PowerPac 3000 165-5056 165-5057 165-5058 165-5059 165-5060 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 3: Table Of Contents

    POWER Table of Contents TABLE OF CONTENTS Safety ..............................Section 1.0 Introduction......................... 1.1 Overview ........................1.2 Features ........................1.3 Unpacking ........................Section 2.0 Control Features......................2.1 Front Panel controls ....................2.2 Rear Panel ........................Section 3.0 Manual Mode Operation..................... Section 4.0 Program Mode Operation ..................4.1 Create and Run a Method...................

  • Page 4: Safety

    2. Always connect the power supply to a 3-prong, grounded AC outlet, using the 3-prong AC power cord provided with the power supply. 3. Bio-Rad electrophoresis cells have molded two-prong plugs which are inserted into the power sup- ply’s high voltage output jacks. These plugs have been I.E.C. 1010 certified for safety compliance for use with PowerPac power supplies.
  • Page 5 I.E.C. 1010 certification, and create a potential safety hazard for the user. Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad or an authorized agent.

  • Page 6: Section 1.0 Introduction

    Adjustable from 1 to 400 watts (W) in increments of 1 W The PowerPac 1000 and PowerPac 3000 are fully programmable, with the capacity to store up to 9 separate methods, with each method consisting of up to 9 steps. PowerPac default limit values for voltage, current, and power are displayed when the unit first is turned on.

  • Page 7: Features

    Unpack the power supply. PowerPac power supplies are shipped with the following: • Power supply unit. • 3-prong, AC power cord. • User Manual. If any part is missing or damaged, contact Bio-Rad Laboratories immediately. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 8: Section 2.0 Control Features

    Control Features POWER 2.0 CONTROL FEATURES 2.1 FRONT PANEL CONTROLS Table 1. Front Panel Controls POWER POWER FAIL DETECT MANUAL >> PROGRAM >> EXIT – + – + – + – + Description Start key: Starts a run. Stop key: Stops the run which is currently in progress. The end of run parameters are displayed.

  • Page 9: Rear Panel

    POWER Control Features Table 1. (continued) Front Panel Controls Description : Allow you to select that parameter or execute that command on the Soft keys DETECT graphic display which is located adjacent to its key. MANUAL >> Temperature mode is available only with the PowerPac 3000, and it requires PROGRAM >>...

  • Page 10: Section 3.0 Manual Mode Operation

    Manual Mode Operation POWER 3.0 MANUAL MODE OPERATION PowerPac power supplies may be operated automatically using pre-defined methods, or may be run manu- ally at a specified voltage, current, or power. The PowerPac 3000 may also be programmed to run at a specified temperature.
  • Page 11 If you make a mistake when entering a value, press the key and re-enter the value. PowerPac 1000 Constant voltage: The default limits are 500 mA and 250 W. Constant current: The default limits are 1000 V and 250 W.
  • Page 12 Manual Mode Operation POWER Table 2. (continued) Manual Mode Operation Step Procedure Description Select one of the following: a. TIME a. TIME: To specify automatic time control. You may then enter a time from 1 minute to 99 hours and 59 minutes. After you have entered a value, press to start the run.

  • Page 13: Manual Mode Flow Chart

    POWER Manual Mode Operation Manual Mode (Optional) POWER FAIL DETECT MANUAL >> PROGRAM >> CONSTANT >> (Or) CONSTANT >> (Or) CONSTANT >> CONSTANT V CONSTANT W CONSTANT mA CONSTANT xxxx V xxxx V xxx mA CONSTANT xxx mA xxx W xxx W CONSTANT TIME/V-HOUR >>...

  • Page 14: Section 4.0 Program Mode Operation

    Program Mode Operation POWER 4.0 PROGRAM MODE OPERATION There are four selections under Program mode: • Create a method • Edit a method • Run a method • View a method Each of these selections is discussed in the following sections. To start, follow the procedure in Table 3, Preliminary Setup Procedure.

  • Page 15: Create And Run A Method

    To change these limits, select the limit to be changed and enter if desired. a value using the numeric keypad. PowerPac 1000 Constant voltage: The default limits are 500 mA and 250 W. Constant current: The default limits are 1000 V and 250 W.
  • Page 16 Program Mode Operation POWER Table 4. (continued) Procedure for Creating and Running a Method Step Procedure Description Program the next step or Select either of the following: save the program. a. If you are defining another step, select . Repeat ADD STEP procedures 3 through 6 above.

  • Page 17: Create Method Flow Chart

    POWER Program Mode Operation Program Mode: Creating a Method (Optional) POWER FAIL DETECT MANUAL >> PROGRAM >> CREATE >> EDIT >> RUN >> VIEW >> STEP 1 CONSTANT >> (Or) CONSTANT >> (Or) CONSTANT >> CONSTANT V CONSTANT W CONSTANT mA xxxx V xxxx V CONSTANT...

  • Page 18: Edit And Run A Method

    Program Mode Operation POWER 4.2 EDIT AND RUN A METHOD The Edit selection of the Program mode allows you to change an existing method. Table 5. Procedure for Editing and Running a Method Step Procedure Description Select from the This procedure assumes you have set up the unit as discussed PROGRAM main menu.
  • Page 19 POWER Program Mode Operation Table 5. (continued) Procedure for Editing and Running a Method Step Procedure Description Enter the method number. Make your selection based on the following: Note: If you select a • To save the original method and all its parameter values, method number different select a number different from that of the method being edit- from that which you select...

  • Page 20: View A Method

    Program Mode Operation POWER 4.3 VIEW A METHOD This selection allows you to quickly view the contents of a stored method. Table 6. Procedure for Viewing a Method Step Procedure Description Select from the This procedure assumes you have set up the unit as discussed PROGRAM main menu.

  • Page 21: Run A Method

    POWER Program Mode Operation 4.4 RUN A METHOD This procedure allows you to quickly select and run a stored method. Table 7. Procedure for Running a Stored Method Step Procedure Description Select from the This procedure assumes you have set up the unit as discussed in PROGRAM main menu.

  • Page 22: Display While Programmed Method Is In Progress

    Program Mode Operation POWER During operation of the power supply, the LCD display shows the operating parameter values, as shown in Figure 5. POWER TIME 00:10 800 V 300 mA 240 W – + – + – + Legend 1. Status of the run. 2.

  • Page 23: Display Upon Completion Of Method

    POWER Program Mode Operation After the programmed time or volt-hours, the power supply automatically continues with the next step or if there is none, it stops. When the method stops running, the final operating parameters, along with the origi- nal set parameters of the last step completed, are displayed, as shown in figure 6. POWER END: 574V/174mA/100W SET: 1000V/500mA/100W...

  • Page 24: Section 5.0 Temperature Mode Operation (Powerpac 3000 Only)

    Temperature Mode Operation POWER 5.0 TEMPERATURE MODE OPERATION (POWERPAC 3000 ONLY) Temperature mode allows you to operate an electrophoresis apparatus at a constant temperature. Constant temperature is extremely useful for performing DNA sequencing or single stranded conformational polymor- phism (SSCP) analysis. The PowerPac 3000 can maintain gel temperatures anywhere from 0°C to 90°C with an accuracy of ±...
  • Page 25 POWER Temperature Mode Operation Table 8. (continued) Temperature Mode Operation Step Procedure Description Attach the temperature Before attaching the temperature probe to the front glass plate of probe to the apparatus. the electrophoresis apparatus. be sure the glass plate is clean to guarantee proper probe attachment.
  • Page 26 Temperature Mode Operation POWER Table 8. (continued) Temperature Mode Operation Step Procedure Description Enter the desired tempera- The temperature range is 0°C to 90°C. ture in degrees centigrade. Select Press the appropriate soft key. The power supply will always run POWER constant power while operating in temperature mode, unless there is cross-over.

  • Page 27: Examples Of Power Requirements

    Table 9. Examples of Power Requirements ® The following table shows approximate power requirements to maintain 50°C using a Bio-Rad Sequi-Gen sequencing apparatus with 0.4 mm spacer, 5% gel and 1xTBE buffer. Dimensions 21 x 40 cm 21 x 50...

  • Page 28: Temperature Mode Flow Chart (Powerpac 3000 Only)

    Temperature Mode Operation POWER Temperature Mode (Optional) POWER FAIL DETECT MANUAL >> PROGRAM >> TEMPERATURE >> ENTER TEMPERATURE IN DEGREE C: °C SET POWER >> 3000 CONSTANT TIME/V-HOUR >> SET: 45 °C / xxx W TIME >> (Or) V-HOUR >> PRESS TO RUN (Optional)

  • Page 29: Section 6.0 Maintenance And Troubleshooting

    POWER Maintenance and Troubleshooting 6.0 MAINTENANCE AND TROUBLESHOOTING 6.1 MAINTENANCE PowerPac power supplies require very little maintenance to assure reliable operation. During normal opera- tion, spills and splashes may cause residues to form on the case. Unplug the power supply. Use a damp cloth to wash down the outer case.
  • Page 30 CORRECT THE FAULT • Reset the power supply by pressing CE. PRESS CE TO RESET Calibration has been lost. Call Bio-Rad for assistance. VOLT mA NOLOAD ARE NOT CALIBRATED Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 31 POWER Maintenance and Troubleshooting Error Message Solution PowerPac 1000 only: , or The voltage, current, or power output has exceeded the power OPERATING OVER 1000 V , or supply’s maximum output capability. OPERATING OVER 500 mA • Check cell for short circuits.

  • Page 32: Appendix A. Specifications

    Line Regulation: PowerPac 1000: ±1% at 1000 V and 225 W from 90 - 132 V AC and 198 - 264 V AC PowerPac 3000: ±1% at 3000 V and 400 W from 90 - 132 V AC and 198 - 264 V AC Load Regulation: PowerPac 1000: ±1% at 1000 V for a 50% change in output load...
  • Page 33 Overload/short circuit protection: Alarm display and power down upon short circuit detection PowerPac 1000: 3000 V DC rms isolation from high voltage outputs to earth ground PowerPac 3000: Internal fusing on both hot and neutral 4000 V DC rms isolation from high voltage outputs to earth ground...
  • Page 34 0 - 95% relative humidity, non-condensing Dimensions: 29 (L) x 28 (W) x 11 (H) cm; unit is stackable Weight: PowerPac 1000: 3.1 kg PowerPac 3000: 3.7 kg Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 35: Appendix B. Warranty And Ordering Information

    The PowerPac power supply is warranted for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad Laboratories will replace the defective parts with- out charge. However, the following defects are specifically excluded: 1.
  • Page 36 Temperature Probe 165-5059 PowerPac 3000 with temperature probe, 100/120 V 165-5060 PowerPac 3000, with temperature probe, 220/240 V PowerPac 1000 165-5054 PowerPac 1000, 100/120 V 165-5055 PowerPac 1000, 200/240 V PowerPac 300 165-5050 PowerPac 300, 100/120 V 165-5051 PowerPac 300, 200/240 V...
  • Page 37 Laboratories Life Science Web site www.bio-rad.com Bio-Rad Laboratories Main Office 2000 Alfred Nobel Drive, Hercules, CA 94547, Ph. (510) 741-1000, Fx. (510) 741-5800 Group Also in: Australia Ph. 02 9914 2800, Fx. 02 9914 2889 Austria Ph. (01) 877 89 01, Fx. (01) 876 56 29 Belgium Ph. 09-385 55 11, Fx. 09-385 65 54 Brazil Ph.
  • Page 38 Table of Contents POWER Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 39 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

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Powerpac 3000

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