Adjusting How Images Are Displayed; Light Intensity Histogram - BIO RAD ChemiDoc Touch User Manual

Adjusting How Images Are Displayed

Adjusting How Images Are Displayed
Image Lab™ Touch software optimizes images based on the range of intensity levels in the
image and the known behavior of the applications. Use this optimized image as a starting
point. Use the histogram scale and the grayscale curve settings in the Transform dialog
box to adjust the image brightness and contrast as necessary.
Important: The transform settings change only the appearance of the image, not the
underlying data.
Note: Chemiluminescent images taken in SAM mode are displayed with a fixed
transform so you can compare them with each other. The intensity is set to the bottom
third of the full range and the gamma is set to .75. The fixed transform enables you to
distinguish accurately the differences between the images in one SAM acquisition and
select the best one.

Light Intensity Histogram

Transform adjusts image brightness and contrast, optimizing the image display so faint
details can be seen. The minimum to maximum range varies depending on the light and
dark values present in the image. Adjustments to brightness and contrast do not change
the data. They change only the way the data are displayed.
The frequency distribution histogram shows the total data range in the image and the
amount of data at each point in the range.
Tap Auto to return to the default setting. The lightest part of the image is set to the minimum
intensity, and the darkest is set to the maximum.
Use the Low and High sliders to narrow the displayed grayscale range.
Reducing gamma highlights the background, nonspecific binding, and faint bands.
Increasing gamma reduces the background and nonspecific binding and highlights the
intense bands.
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