Agilepulse™ Id System Components And Set-Up; Introduction; The Agilepulse Waveform Generator; Figure 1: Agilepulse Waveform Generator - BTX AgilePulse ID User Manual

AgilePulse ™

Introduction

The AgilePulse™ system provides the capability of delivering DNA
vaccines to the dermis. The system is designed for easy set-up and
operation.
Specifications are presented on page 27.
The AgilePulse™ ID system is composed of three subsystems:
• Touchscreen controlled AgilePulse Waveform Generator
• Intradermal Array (ID) parallel, needle electrodes
• User Manual
Optional equipment available includes:
• Foot Pedal (47-0420)
• Adapter box for electrodes with 4mm banana cables
(47-0090 or 47-0091, please see Ordering Information
page 27 for details)
• 2-Needle Array, 5 mm gap, pkg. of 6 (45-0121)
• 2-Needle Array Handle, for 5 mm gap (45-0206)
The AgilePulse ID
Waveform Generator
The Waveform Generator (Figure 1) is composed of a computer and
high voltage pulsing circuits. The computer runs mobile Windows®
and is operated by a touch screen. On the front panel there is
a power switch, a touch screen, an emergency stop switch, an
electrode connector and two USB ports. All User inputs are entered
via the touch screen or by connecting a keyboard to one of the USB
ports. The system may be operated by using an optional foot pedal.
On the back panel there is a line/mains power connector.
The high voltage pulsing circuits are controlled by a microcontroller.
The microcontroller accepts user inputs from the touch screen and
produces the pulse waveforms. A second microcontroller is used
as an independent audit of the waveforms produced. If the audit
function detects a deviation from the desired pulse protocol, the
system immediately terminates pulsing. The system also digitizes
the output of the pulse voltage and pulse current monitors and
calculates the skin resistance of the tissue on each pulse. This is
a significant quality control function that indicates the vaccine is
being delivered properly. After the vaccine is delivered, a log of all
parameters is saved in Internal Memory and on a USB memory key,
if one is inserted.
5
ID System Components & Set-Up
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com

Figure 1: AgilePulse Waveform Generator

AgilePulse Waveform Generators come in two varieties,
specialized for pulse accuracy in relation to electrical resistance
of the sample tissue. AgilePulse ID is recommended for
electroporation of high resistance tissue, such as skin and tibialis
muscle. AgilePulse IM is recommended for low resistance tissue
such as quadriceps muscle. Please refer to Figure 2, AgilePulse
Waveform Generator Selection Guide, for details.
AgilePulse Waveform Generator Selection Guide
Tissue Type Approximate
Tissue Load Range
Skin, intradermal 1100 to 2200 Ohms
electroporation
Tibialis muscle 800 to 1500 Ohms
Quadriceps, 200 to 400 Ohms
small animal
Quadriceps, 80 to 250 Ohms
large animal
Recommended
Waveform
Generator
AgilePulse ID
AgilePulse ID
AgilePulse IM
AgilePulse IM
Figure 2