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Summary of Contents for BTX AgilePulse ID
- Page 1 AgilePulse ™ In Vivo Gene Delivery System USER’S MANUAL Publication 015-101444 Rev 2.0...
- Page 2 A Message from BTX Thank you for investing in an AgilePulse system. Since its founding in 1983, the main focus of BTX has been in the area of applying controlled electric fields for genetic engineering applications. Because of this, we quickly established a reputation as the technological leader in the fields of electroporation and electrofusion.
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Page 3: Table Of Contents
General Electroporation Discussion AgilePulse™ Electroporation 20–22 AgilePulse In Vivo/Ex Plant Electroporation Protocol 23–24 (Animal Skin/Tibialis Muscle) Specifications Maintenance Fuse Replacement Electrode Care and Cleaning Ordering Information References 28–29 AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... - Page 4 Figure 22: Electroporation Figure 23: Transmembrane Voltage Figure 24: Two Needles per Row Field Figure 25: Electrical Field Coverage Figure 26: Bleb Formation Figure 27: Fuse Replacement AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com...
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Page 5: Warranty Information
Without limiting the generality atmosphere such as an anesthetic mixture with air, oxygen, or of the foregoing, BTX shall not be liable for any claims of any kind nitrous oxide. whatsoever, as to the equipment delivered or for non-delivery of equipment, and whether or not based on negligence. -
Page 6: Safety Information
Make sure all connections are made properly and securely. Any signal wire connections to the unit must be no longer than three The BTX AgilePulse Systems are intended for laboratory use meters. only and can be used in research and development applications. -
Page 7: Agilepulse™ Id System Components And Set-Up
The computer runs mobile Windows® of the sample tissue. AgilePulse ID is recommended for and is operated by a touch screen. On the front panel there is... -
Page 8: Front Panel
The IDA electrode is described in more detail on page 8. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 9: Setting Up The System
GREEN indicating the system is ready for use (Figure 5). If the NEXT button turns RED, then the internal checks failed (Figure 6). The system cannot be used if the button is red. Contact BTX Customer Service for assistance. -
Page 10: System Operation
Once the transfectant is ready for administration, remove the Figure 9: Attaching IDA Tip to Handle protective cover from the IDA Tip taking care not to disconnect the tip from the handle. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 11: Waveform Generator
Password entry is masked with “*” characters in order to provide increased security. When the User name and Password have been entered, touch OK to log in. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 12: Experiment Delivery Screen Functions And Operation
DONE button pressed. In this case, an early termination message will appear in the data log and the system reset. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 13: Experiment Delivery Logs
• Monitor width of each pulse • • • Monitor interval of each pulse • • Pre-pulse Load Reading • System Serial Numbers • System Software Versions AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 14: Text Log
9.975 20.000 19.947 0.144 10.000 9.975 20.000 0.000 Load Reading: Date Stop: 2/12/2007 2:16:40 PM (2/12/2007 10:16:40 PM GMT) Time Elapsed: 00:00:18 Figure 17: Text Log Screen AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 15: Csv Data
Microsoft Excel® for further user manipulation. As an example in Figure 18, a user chart was made to display the monitored amplitude samples of three 100 microsecond pulses run at 5 kHz. In this case, each pulse contains eight amplitude samples. Figure 18: Sample Amplitude Analysis AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 16: Delivery Completion Return Codes
System Operation Delivery Completion Return Codes Return codes are available following system shutdown describing how it took place. Please contact BTX technical support to resolve the errors. Return Code Description Successful completion (Normal Operation) Emergency Flash memory storage failure Waveform protocol data integrity verification error... -
Page 17: Software Maintenance Mode
The administrator also has the ability to change a user’s password. This is ideal for situations in which the user forgets their password and thus need the password to be reset in order to log in again. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 18: Log Retrieval
Welcome Screen. If modifications are made and system power is turned off while still viewing the screen, all changes will be lost. Caution AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 19: Application
Many Many Deliver viral vector with Muscle • Immune system reacts to viral hypodermic needle into vector, inefficient when boosting muscle • Pain 2 out of 10 AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 20: Agilepulse™ Id Tutorial
(amplitude, duration, number and inter-pulse interval). AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 21: Figure 23: Transmembrane Voltage
To produce a TMV of 1 volt across the membrane of a cell with 7 µm radius, the required electric field intensity is: ⁄ 950 volts / cm 7 *10 AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 22: Agilepulse™ Electroporation
Group Pulse Amplitude Width Interval Interval Number 450 V 0.05 ms 0.2 ms 50 ms (1125 V/cm) 110 V 10 ms 20 ms 50 ms (275 V/cm) AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 23: Figure 24: Two Needles Per Row Field
The AgilePulse needle electrodes are specifically designed to produce near uniform electric fields in the treatment volume. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 24: Figure 26: Bleb Formation
There is a significant tradeoff between wider row spacing and larger delivery volume and pulse amplitude. As pulse amplitude increases so does pain of the applied pulse voltage. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 25: Agilepulse In Vivo/Ex Plant Electroporation Protocol
Note: These are recommended electroporation settings. Reducing the pulse interval time below 50 ms in group 2, can lead to error 19. The interval time can be increased to 300 ms or more, if the error recurs. Please contact BTX technical support if an error persists. - Page 26 • For muscle electroporation insert needle tip into the muscle at desired depth and slowly inject DNA into site. • Inject 20 to 30 μl transfectant solution. • Repeat the procedure on the other sites if necessary. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com...
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Page 27: Specifications
20% to 80% RH, non-condensing Storage Humidity 20% to 80% RH, non-condensing Mode of Operation Continuous Classification Class I Ingress IP2X Installation Category II * A lldataautomaticallystoredininternalmemoryandmaybedownloadedtoanexternalUSBKey. MaximumDataLogsstoredandretrievablefrominternalflashmemory>20,000. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 28: Maintenance
• Rinse well with sterile lab grade water. • Spray with 70% alcohol. • Air dry. • Replace needle protection cap for storage until next use. Figure 27: Fuse Replacement AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 29: Ordering Information
1 Ea For generators with Serial No. AP-4069-ID or later. 1 Ea Service Offerings 45-9996 AgilePulse 1-Year Extended Warranty 1 Ea 45-0909 AgilePulse System Validation Certificate 1 Ea AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... -
Page 30: References
Daftarian MP, Vosoughi A, Lemmon V. Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies. MethodsMolBiol. 2014;1121:337-346. Davtyan H, et al. BTX AgilePulse(TM) System is an Effective Electroporation Device for Intramuscular and Intradermal Delivery of DNA Vaccine. Curr Gene Ther. 2014;14(3):190-199. -
Page 31: References
Wolf H, Rols M P, Boldt E, Neumann E, Teissie J. Control by pulse parameters of electric field mediated gene transfer in mammalian cells. BiophysJ. 1994;66:524-531. Zerbib D, Amalric F, Teissie J. Electric field mediated transformation: isolation and characterization of a TK+ subclone. BiochemBiophysResCommun. 1985;129:611-618. AgilePulse ID In Vivo Gene Delivery System Publication 015-101444 Rev 2.0 • www.btxonline.com... - Page 32 Contact Us US & Canadian Customers International Customers phone 508.893.8999 To locate a distributor 508.429.5732 near you, please visit our e-mail support@hbiosci.com website at www.btxonline.com website www.btxonline.com...
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