Agilepulse™ Electroporation - BTX AgilePulse ID User Manual

AgilePulse ™
AgilePulse™ ID Electroporation
In pages 17 to 19 general concepts of electroporation were
presented. This section presents the types of waveforms and
electrodes available for use in the AgilePulse system.
Waveforms
Traditional in vivo electroporation uses four to six rectangular
pulses that are 100 microseconds in duration at a rate of one per
second. Thus the total treatment time is four to six seconds. More
advanced waveforms such as the PulseAgile have been found
to be more effective in eliciting T-cell and antibody responses
simultaneously when delivering DNA vaccines (Roos et al, 2006,
Vertuani et al 2009, Bråve et al 2009). A PulseAgile waveform
consists of various pulse groups with different characteristics from
group to group. For example, the study by Dr. Roos found the
optimum waveform for induction of high gene expression and high
induction of antigen-specific T cells to be:
Group Pulse Pulse
Amplitude Width
1 450 V 0.05 ms 300 ms
(1125 V/cm)
2 110 V 10 ms
(275 V/cm)
The total treatment time for this waveform is 2.98 seconds.
(Waveforms for clinical use must be optimized in pre-clinical
studies.)
Another more recent study by Roos et al (Mol. Ther., 2009)
developed a variant of the PulseAgile waveform described above.
This waveform is called Fast PulseAgile (PAfast) and differs only in
the length of the pulse intervals:
Group Pulse Pulse
Amplitude Width
1 450 V 0.05 ms 0.2 ms
(1125 V/cm)
2 110 V 10 ms
(275 V/cm)
20
ID Tutorial
Pulse Group Pulse
Interval Interval Number
500 ms 2
300 ms 500 ms 8
Pulse Group Pulse
Interval Interval Number
50 ms 2
20 ms 50 ms 8
AgilePulse ID In Vivo Gene Delivery System
Publication 015-101444 Rev 2.0 • www.btxonline.com
The total treatment time for this waveform is 0.27 seconds (this
waveform is currently tested in three clinical studies). When the
pulses are delivered in less than half a second there is only one
muscle contraction and tolerability is highly improved.
The AgilePulse system has the following waveform settings
available:
Pulse Pulse Pulse
Amplitude Width Interval*
50 to 0.050 to 0.2 to
300 V 10 ms 1000 ms
310 to 1000 0.050 to 0.2 to
V 1 ms 1000 ms
*Limited to 50% Duty Cycle, i.e., (Pulse Width/Pulse Interval) less than or
equal to 0.5
Three groups may be used. A group is 1 to 10 pulses with the same
parameters. The system software automatically concatenates each
group.
Maximum Number
Duty Cycle of Pulses
50% 10
50% 10